Synergistic Effect of Monofloral Honeys and Essential Oils against Pseudomonas aeruginosa

Laïd Boukraâ *

Laboratory of Research on Local Animal Products, Ibn-Khaldoun University of Tiaret, Algeria and Mohammad Hussein Al Amoudi Chair for Diabetic Foot Research, King Abdulaziz University, Jeddah, Saudi Arabia.

Hasan A Alzahrani

Mohammad Hussein Al Amoudi Chair for Diabetic Foot Research, King Abdulaziz University, Jeddah, Saudi Arabia and Department of Surgery, Faculty of Medicine, KAU, Jeddah, Saudi Arabia

Fatiha Abdellah

Laboratory of Research on Local Animal Products, Ibn-Khaldoun University of Tiaret, Algeria.

Balkees Bakhotmah

Mohammad Hussein Al Amoudi Chair for Diabetic Foot Research, King Abdulaziz University, Jeddah, Saudi Arabia and Department of Nutrition & Food Sciences, Art & Design College, KAU, Jeddah, Saudi Arabia.

Si Mohamed Hammoudi

Laboratory of Research on Local Animal Products, Ibn-Khaldoun University of Tiaret, Algeria.

*Author to whom correspondence should be addressed.


Abstract

Aims: The emergence of pathogenic bacterial strains with resistance to commonly used antibiotics has necessitated a search for novel types of antibacterial agents. The main objective of this study was to evaluate the synergistic action between honeys and the essential oils (EOs) against Pseudomonas aeruginosa (ATCC 27853).
Study Design and Methodology: In the first step, the minimum inhibitory concentration (MIC) of each honeys and Eos were determined. In the second step, lower concentrations of honey than the MIC were mixed with a set of sub-MIC of EOs and then added to media to determine the minimum synergistic inhibitory concentration (MSIC).
Place of Study: Laboratory Research onof Local Animal Products, Ibn-Khaldoun University, Tiaret, Algeria.
Results: The results indicated that the essential oils and all varieties of honey were effective against P. aeruginosa. The effectiveness was correlated to the botanical origin of honey and EOs. Wild carrot honey and Origanum vulgaris EOs were the most effective against the tested bacteria with a MIC of 8% and 2% respectively. Adding EOs to honey decreases the MIC values and the isobolographic representation shows a synergistic action between the EOs and all varieties of honey.
Conclusion: The current prevalence of antibacterial resistant species has led to a re-evaluation of the therapeutic use of ancient remedies, including honey and EOs, which may receive renewed recognition as wound and burn healers.

Keywords: Antibacterial, honey, essential oils, synergy, Pseudomonas aeruginosa.


How to Cite

Boukraâ, L., Alzahrani, H. A., Abdellah, F., Bakhotmah, B., & Hammoudi, S. M. (2013). Synergistic Effect of Monofloral Honeys and Essential Oils against Pseudomonas aeruginosa. Microbiology Research Journal International, 3(4), 564–573. https://doi.org/10.9734/BMRJ/2013/5331

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