Phytochemical Study and Antimicrobial Activity of Bark Extracts of Ceiba pentandra (L.) Gaertn. (Bombacaceae) from Côte d’Ivoire on Antibiotic Resistant Staphylococcus aureus and Pseudomonas aeruginosa

Golly Koffi Julien *

Université Felix Houphouët Boigny, Département de Biochimie, Laboratoire de Biotechnologies/ pharmacologies des substances naturelles, 01 BP V 34 Abidjan 01, Côte d’Ivoire. & Université Felix Houphouët Boigny, Laboratoire de Bactériologie – Virologie de l’Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d’Ivoire. & Institut National Polytechnique HOUPHOUET-BOIGNY de Yamoussoukro, Département de Génie Chimique et Agro-Alimentaire, Laboratoire des Procédés Industriels, de Synthèse et des Energies Nouvelles (LAPISEN)), (BP 1093 Yamoussoukro, Côte d’Ivoire

Siaka Sorho

Institut National Polytechnique HOUPHOUET-BOIGNY de Yamoussoukro, Département de Génie Chimique et Agro-Alimentaire, Laboratoire des Procédés Industriels, de Synthèse et des Energies Nouvelles (LAPISEN)), (BP 1093 Yamoussoukro, Côte d’Ivoire).

Soro Yaya

Institut National Polytechnique HOUPHOUET-BOIGNY de Yamoussoukro, Département de Génie Chimique et Agro-Alimentaire, Laboratoire des Procédés Industriels, de Synthèse et des Energies Nouvelles (LAPISEN)), (BP 1093 Yamoussoukro, Côte d’Ivoire).

Guessennd Nathalie

Université Felix Houphouët Boigny, Laboratoire de Bactériologie – Virologie de l’Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d’Ivoire.

Dosso Mireille

Université Felix Houphouët Boigny, Laboratoire de Bactériologie – Virologie de l’Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d’Ivoire.

Djaman Allico Joseph

Université Felix Houphouët Boigny, Département de Biochimie, Laboratoire de Biotechnologies/ pharmacologies des substances naturelles, 01 BP V 34 Abidjan 01, Côte d’Ivoire.

*Author to whom correspondence should be addressed.


Abstract

One of the health problems in recent decades is the emergence of multi-antibiotic resistant bacteria. Among the solutions to this concern, the secondary metabolites of medicinal plants seem to be privileged. In this context, our work is devoted to the phytochemical study and evaluation of antibacterial activity of bark extracts of Ceiba pentandra from Ivorian Pharmacopoeia on methicillin resistant S. aureus and on imipenem and ceftazidime resistant P. aeruginosa. The phytochemical study shows that bark of Ceiba pentandra harvested in the center of Côte d’Ivoire contains alkaloids, flavonoids, saponins, tannins, steroids, terpenoids and cardiac glycosides. Microbiological testing showed that in liquid medium, ethyl acetate extract is active on all studied bacteria. Minimum Inhibitory Concentration (MIC) range from 0.78 to 6.25 mg/mL and Minimum Bactericidal Concentration (MBC) from 1.04 to 8.33 mg/mL. Purification of ethyl acetate extract had no influence on its activity against the studied bacteria. In fact, the most active fraction has MIC ranging from 0.52 to 6.25 mg/mL and MBC from 1.04 to 10.42 mg /mL. According to the ratio MBC /MIC, which was nearly 2, ethyl acetate extract and his active fraction are bactericidal.

Keywords: Ceiba pentandra, Phytochemistry, Staphylococcus aureus, Pseudomonas aeruginosa, resistant


How to Cite

Julien, G. K., Sorho, S., Yaya, S., Nathalie, G., Mireille, D., & Joseph, D. A. (2015). Phytochemical Study and Antimicrobial Activity of Bark Extracts of Ceiba pentandra (L.) Gaertn. (Bombacaceae) from Côte d’Ivoire on Antibiotic Resistant Staphylococcus aureus and Pseudomonas aeruginosa. Microbiology Research Journal International, 9(1), 1–7. https://doi.org/10.9734/BMRJ/2015/18103

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