Morphological Diversity of Fungal Strains Responsible for Dessert Banana Rot, Musa Sapientum (Musaceae) Produced in the South Comoe Region on Ivory Coast

Foba Foba Stéphane Isaac *

Agrovalorisation Laboratory, Agroforestry Training and Research Unit (UFR-AGRO), University Jean Lorougnon GUEDE PB-150, Daloa, Ivory Coast.

Coulibaly Ibourahema

Agrovalorisation Laboratory, Agroforestry Training and Research Unit (UFR-AGRO), University Jean Lorougnon GUEDE PB-150, Daloa, Ivory Coast.

Coulibaly Adja Massagna

Agrovalorisation Laboratory, Agroforestry Training and Research Unit (UFR-AGRO), University Jean Lorougnon GUEDE PB-150, Daloa, Ivory Coast and Laboratory of the Unit of Antibiotics, Natural Substances and Surveillance of Resistance of Microorganisms to Anti-Infectious (ASSURMI), Pasteur Institute of Ivory Coast (IPCI) BP-490, Abidjan-01, Ivory Coast.

Konan Kouakou Ahossi

Agrovalorisation Laboratory, Agroforestry Training and Research Unit (UFR-AGRO), University Jean Lorougnon GUEDE PB-150, Daloa, Ivory Coast.

*Author to whom correspondence should be addressed.


Abstract

Aims: The banana dessert grown in different regions of the world, encounters important difficulties that cause it to lose its marketability especially because of the diseases related to conservation. The objective here is to identify the strains responsible for fungal diseases of bananas when it is stored.

Study Design:  This study was undertaken in order to ensure the competitiveness of the dessert banana from Ivory Coast on the international market which is threatened by the recurrent problem of post-harvest rot.

Place and Duration of Study: Agrovalorisation Laboratory, Agroforestry Training and Research Unit, Université Jean Lorougnon GUEDE Daloa Côte d'Ivoire, between February 2021 and March 2022.

Methodology: The study involved 120 bananas divided into two batches of 10 hands each, one with signs of necrosis and the other with no signs. Banana fragments (crown, epicarp and explant) were deposited on the growing media at several distinct points and slightly embedded in the agar. The resulting colonies were transplanted successively until a pure strain was obtained from a single mushroom colony per petri dish.

Results: A total of 11 different genera were identified from 105 isolates. 57 are from necrotic bananas and 36 from bananas with no signs of necrosis. Fungal strains isolated there are: Trichoderma sp. (15%), Fusarium sp. (1%), Scytalidium sp. (39%), Mucor sp. (1%), Scopulariopsis sp. (1%), Alternaria sp. (4%), Aureobasidium sp. (1%), Aspergillus of the Glaucus group (10%), Cladosporium sp. (2%), Pseudallescheria sp. (6%) and Chrysosporium sp. (20%). No strains of the genus Colletotrichum musea responsible for anthracnose that can develop on both green and ripe fruit have been isolated. However, morphological characterization has not among to identify several other species (12) especially those not sporulating.

Conclusion: This diversity of isolated strains in this work is identical to that most frequently isolated and cited in the literature.

Keywords: Dessert banana, fungal strains, Isolates, necrosis, Ivory Coast


How to Cite

Isaac, F. F. S., Ibourahema, C., Massagna, C. A., & Ahossi, K. K. (2022). Morphological Diversity of Fungal Strains Responsible for Dessert Banana Rot, Musa Sapientum (Musaceae) Produced in the South Comoe Region on Ivory Coast. Microbiology Research Journal International, 32(10), 20–31. https://doi.org/10.9734/mrji/2022/v32i101348

Downloads

Download data is not yet available.

References

Ewané CA, Lassois L, Brostaux Y, Lepoivre P, De Lapeyre De BL. The susceptibility of bananas to crown rot disease is influenced by geographical and seasonal effects. In: Canadian Journal of Plant Pathology. 2013; 35(1): 27-36.

FAOSTAT. Food and Agriculture Organization of the United Nations. Agricultural Statistics; 2012. Accessed September 02, 2021.

Available:Bananas.https://www.faostat3.fao

Ewané CA. Study of the biodiversity of the microflora of banana washing water in a packing station and its relationship with the manifestation of crown rot in Cameroon. University Faculty of Agricultural Sciences of Gembloux. Belgium. 2007;140.

Bayère L. Evaluation of post-harvest diseases of the Grande Naine dessert banana (Musa acuminata L.) in production and packaging areas in Côte d'Ivoire. 2016;52.

Essis B, Kobenan K, Traoré S, Koné D, Yatty J. Laboratory sensitivity of Mycosphaerella fijiensis responsible for black Sigatoka of bananas to fungicides commonly used in Ivorian banana plantations. Journal of Animal & Plant Sciences. 2010;7(2):822-833.

FAOSTAT. Banana statistics 2011. 2011. Accessed September 2, 2021.

Available: www.fao.org/economic/est/en.

Tripathi L, Mwangi M, Abele S, Aritua V, Tushemereirwe WK, Bandyopadhyay R. Xanthomonas wilt: A threat to banana production in East and Central Africa. Plant Disease. 2009;93 (5):440-451.

Kumar PL, Selvarajan R, Iskra-Caruana M-L, Chabannes M, Hanna R. Biology, etiology and control of virus diseases of banana and plantain. Advances of Virus Research.

DOI: 10.1016/bs.aivir.2014.10.006. 2014; 91:229-269.

Zandjanakou-Tachin M, Ojiambo PS, Vroh-Bi I, Tenkouano A, Gumedzoe YM, Bandyopadhyay R. Pathogenic variation of Mycosphaerella species infecting banana and plantain in Nigeria. Plant Pathology. 2013;62:298308.

Botton B, Breton A, Fèvre M, Gauthir S, Guy PH, Larpent JP, Reymond P, Sanglier JJ, Vayssier Y, Veau P. Useful and harmful molds, Industrial Importance, 2nd Edition, Masson, Collection Biotechnologies. 1990;34-42.

Guiraud JP. Food microbiology. Dunod, paris: p.310-321. OEPP/EPPO Bull. 1998; (31):505 - 507.

Kébé IB, Mpika J, N'Guessan KF, Hebbar PK, Samuel SGS, Severin A. Isolation and identification of microorganisms indigenous to cocoa plantations in Côte d'Ivoire and demonstration of their antagonistic effects against Phytophthora palmivora, agent of brown pod rot. Science & Nature. 2009; 6(1):71-82.

Giraud J. Food microbiology. Edition Donod, Paris Mehravar M. & Sardari S; 2011

Huguenin G, Boccas B. Role of some factors in the formation and germination of zoospores in Phytophthora palmivora Bult. Annua Review of Phytopathology 3. 1971; (3):71.

Minagri. Ivorian Ministry of Agriculture; 2015. Accessed 13 October 2015.

Available: http://www.minagri.gov.ci.

Bush EA, Stromberg EL, Hong C, Richardson PA, Kong P. Illustration of key morphological characteristics of Phytophthora species identified in Virginia nursery irrigation water. Online. Plant Health Progress.

DOI: 10.1094/PHP-2006-0621-01-RS. 2006.

Chabasse D, Bouchara JP, De Gentile L, Brun S, Cimon B, Penn P. Molds of medical interest. Training notebook N° 25, Bioforma 230 bd raspail 75014, Paris; 2002.

Chillet M. Hubert O. De Lapeyre de Bellaire L. Relationship between ripening and the development of banana anthracnose caused by Colletotrichum musae (Berck. And Curt.) Arx. Journal of Phytopayhology.2006; 154:143-147.

Demoulin G. Studies of the factors influencing the development of crown rots on export bananas from Cameroon and contribution to the development of an alternative control method. Graduation work. University Faculty of Agricultural Sciences of Gembloux. 2004;78.

Dadzie BK, Orchard JE. Routine post-harvest screening of banana/plantain hybrids: criteria and metbods. International Plant Genetie Resources Institute (TPGRI); 1997.

Available:http://www.cabdirect.org/abstracts/19981606130.html (consulté le 12 Septembre 2021)