Microbiology Research Journal International https://journalmrji.com/index.php/MRJI <p style="text-align: justify;"><strong>Microbiology Research Journal International (ISSN:&nbsp;2456-7043)</strong> is dedicated to publish research papers, reviews, and short communications in all areas of Microbiology such as virology, mycology, parasitology, bacteriology, clinical microbiology, phycology, parasitology, protozoology, microbial physiology, immunology, microbial genetics, medical microbiology, microbial pathogenesis and epidemiology disease pathology and immunology, probiotics and prebiotics</p> en-US contact@journalmrji.com (Microbiology Research Journal International) contact@journalmrji.com (Microbiology Research Journal International) Fri, 04 Sep 2020 10:16:28 +0000 OJS http://blogs.law.harvard.edu/tech/rss 60 Physico-chemical and Bacteriological Characteristics of the Drinking Water of the Aguégués Lake District in the Lower Ouémé Valley - Benin https://journalmrji.com/index.php/MRJI/article/view/30246 <p>Water is an essential resource for life on earth and is the source of several microbiological and toxicological diseases. The Aguégués one of the communes of the lower valley of Ouémé-Benin are very exposed to these type of diseases. Pathogens swarm in a highly polluted environment because of the peculiarity of this predominantly aquatic area. This is what justifies this research, which aims to assess the quality of drinking water for the populations of the Commune des Aguégués. The research technique focused on the field survey which made it possible to identify the different sources of water supply used by the population. Likewise, the physico-chemical and bacteriological quality of these water sources has been determined. These research techniques have made it possible to discover that the population of Aguégués uses several sources of water. Apart from the recommended sources (National Water Company of Benin and Village Water Supply) the populations use other sources of water. These are rainwater consumed in the rainy season by 97% of the households surveyed, surface water used by 78.26% of the population, especially during flood periods for various uses. Physico-chemical and bacteriological analyzes have shown that apart from SONEB waters only available in the districts of Zoungamey and Houédomey, the other sources of water have parameters which do not comply with WHO standards. This justifies the prevalence of diarrheal diseases in the study environment, which represents the second cause of consultation in health centers.</p> Toussaint Cokou Dan, Rodrigue C. Landeou, Boko Dominique, Thierry Azonhe, Bénoît N’ Bessa ##submission.copyrightStatement## https://journalmrji.com/index.php/MRJI/article/view/30246 Fri, 04 Sep 2020 00:00:00 +0000 Isolation, Characterization, and Molecular Identification of Indigenous Bacteria from Fermented Almonds (Prunus dulcis) https://journalmrji.com/index.php/MRJI/article/view/30247 <p><strong>Aims:</strong> This study aims to isolate and identify the indigenous bacteria of almonds fermentation.</p> <p><strong>Methods:</strong> Characterization of the indigeneous bacteria are using gram staining, biochemical tests, 16SrRNA gene sequencing, and the antimicrobial activity against <em>Escherichia coli</em> bacteria.</p> <p><strong>Results:</strong> Approximately 28 x 10<sup>6</sup> CFU / mL bacteria were obtained from almonds fermentations with 14 isolates from enrichment results. Three randomly selected isolates were gram-positive rod-shaped with a negative catalase and positive fermentation test. However, one isolate showed positive results on the motility test. The antimicrobial test results from the three randomly selected isolates using the disk diffusion method obtained inhibition zones of 7 mm, 6.7 mm, and 7 mm, respectively. Therefore, by using 16S rRNA gene sequencing, three different microorganisms were found, namely <em>Bacillus subtilis strain</em> IAM 12118, <em>Bacillus Piscis strain</em> 16MFT21, and <em>Bacillus licheniformis strain</em> BaDB27.</p> <p><strong>Conclusion:</strong> It was found that <em>Bacillus subtilis </em>strain IAM 12118, <em>Bacillus Piscis</em> strain 16MFT21, and <em>Bacillus licheniformis </em>strain BaDB27 in almonds fermentation and also can be used as probiotic bacteria.</p> Salwa Nurhasanah, Edy Fachrial, Nyoman Ehrich Lister ##submission.copyrightStatement## https://journalmrji.com/index.php/MRJI/article/view/30247 Thu, 10 Sep 2020 00:00:00 +0000 Detection of Antibiotic Resistance Genes in Some Multiple Antibiotic Resistant E. coli from Apparently Healthy Pregnant Women https://journalmrji.com/index.php/MRJI/article/view/30248 <p>The emerging drug resistance, especially among the <em>Escherichia coli </em>(<em>E.coli</em>) isolates from pregnant women, spread rapidly within the community. Urinary tract infection (UTI) is a well-known bacterial infection posing serious health problem in pregnant women. Also, multi-drug resistance is becoming rampant, and it is of serious public health concern. Treatment of <em>E. coli</em> is now a challenge due to continuous increase in resistance towards commonly prescribed antibiotics, thus posing a threat to treatment. Hence, the aim of the study is to determine antibiotic resistance genes in some multiple antibiotic resistant <em>E.coli</em> from apparently healthy pregnant women in Osun State. A cross-sectional study design was used to collect 150 mid-stream urine samples from apparently healthy pregnant women from March, 2018 to September, 2018. A well structured questionnaire and informed consent were used for data collection. Standard loop technique was used to place 0.001 ml of urine on Cysteine Lactose Electrolyte Deficient (CLED) medium, Blood agar, MacConkey agar and incubated at 37 °C for 24 h. A standard agar disc diffusion method was used to determine antimicrobial susceptibility pattern of the isolates. The molecular detection of the resistant genes was done using PCR techniques. The ages of women enrolled in this study ranges from 22 to 42 years (mean ± standard deviation = 31 ± 4.7 years). <em>Escherichia coli</em> showed high percentage of resistance to ampicillin and low resistance to ciprofloxacin and penicillin. All the <em>E. coli</em> isolates were sensitive to levofloxacin, and most were resistant to Meropenem. Multiple drug resistance was observed in all the isolates. Resistance genes in <em>VIM </em>390bp, <em>bla ctx</em>-<em>M</em> 585bp and <em>TEM </em>517bp were detected in some of the representative <em>E. coli</em> isolates profiled. This study identified the presence of Multi-drug resistance genes in <em>E. coli</em> associated UTI among pregnant women in Osogbo.</p> O. C. Adekunle, A. J. Falade- Fatila, R. Ojedele, G. Odewale ##submission.copyrightStatement## https://journalmrji.com/index.php/MRJI/article/view/30248 Mon, 14 Sep 2020 00:00:00 +0000