Microbiology Research Journal International https://journalmrji.com/index.php/MRJI <p style="text-align: justify;"><strong>Microbiology Research Journal International (ISSN:&nbsp;2456-7043)</strong> is dedicated to publish research papers, reviews, and short communications in all areas of Microbiology such as virology, mycology, parasitology, bacteriology, clinical microbiology, phycology, parasitology, protozoology, microbial physiology, immunology, microbial genetics, medical microbiology, microbial pathogenesis and epidemiology disease pathology and immunology, probiotics and prebiotics, veterinary microbiology, environmental microbiology, microbial ecology, microbially-mediated nutrient cycling, geomicrobiology, microbial diversity and bioremediation, evolutionary microbiology, enzymology, industrial microbiology, aeromicrobiology, food microbiology, molecular and cellular microbiology, entomology, biomedical sciences, pharmaceutical microbiology, agricultural microbiology, soil microbiology, zoology, endocrinology, toxicology, water microbiology, generation microbiology and nano microbiology. By not excluding papers based on novelty, this journal facilitates the research and wishes to publish papers as long as they are technically correct and scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled, OPEN peer-reviewed, open-access INTERNATIONAL journal.</p> en-US contact@journalmrji.com (Microbiology Research Journal International) contact@journalmrji.com (Microbiology Research Journal International) Thu, 22 Feb 2024 08:06:15 +0000 OJS http://blogs.law.harvard.edu/tech/rss 60 Effects of Weed Management on Soil Microbiological Properties in Groundnut Production https://journalmrji.com/index.php/MRJI/article/view/1416 <p>To quantify the efficacy of effective weed control methods and spacing of groundnut on soil microbiological properties, a field experiment was conducted between 2009 and 2010 at the Teaching and Research Farm of the University of Agriculture, Abeokuta, a sub-humid region of southwestern Nigeria. Treatments were five levels of weed control (codal gold 1.6 kg a.i/ha, codal gold 1.6 kg a.i/ha + hoe weeding, codal gold 2.4 kg a.i/ha, hoe weeding and a control (weedy check); two levels of spacing (15 cm and 25 cm) and eight levels of weeding intervals on soil microbial biomass, nodulation, the biomass of root and shoot as well as yield of groundnut in a split-plot design fitted into a randomized complete block design. The results indicated that both levels of spacing have no significant effect on the soil microbiological parameters, groundnut biomass production, nodulation and yield. So, the levels of weed control have no significant effect on groundnut biomass production and nodulation but showed a significant effect on microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), total fungal count, pod count and pod weight of groundnut with plots kept weedy throughout the experiment at 25 cm intra row spacing having 34 % MBC more than plots kept weed free throughout the experiment at the same spacing.</p> Adigun M. O., Babalola O. A. Copyright (c) 2023 Adigun and Babalola; This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://journalmrji.com/index.php/MRJI/article/view/1416 Sun, 31 Dec 2023 00:00:00 +0000 Isolation, Characterization, and Selection of Bacterial Endophytes from Soybean (Glycine max) Nodules and Roots in Some Soils of Cameroon for Promoting Growth of Forage Legume Plants https://journalmrji.com/index.php/MRJI/article/view/1417 <p><strong>Aim:</strong> The present study aimed to isolate, characterize and select the most effective bacterial endophytes to enhance soybean growth, biomass, and yield.</p> <p><strong>Methodology:</strong> Seven soil samples were collected from the rhizosphere of forage legumes in fields across three agroecological zones of Cameroon. Bacterial endophytes were isolated from soybean roots and nodules and cultured on nutrient agar. The isolates were screened for the tolerance tests, extracellular enzymatic activity, P-solubilization activity, and IAA production. The best isolates were selected using a two-factor block factorial design. Each treatment was replicated six times.</p> <p><strong>Results:</strong> A total of 85 bacterial endophytes were isolated. Characterization results of 22 preselected isolates revealed an optimal growth temperature of 37°C and a pH range between 6 and 7. Furthermore, the 22 isolates solubilized inorganic phosphate, 7 produced IAA, and 8 exhibited amylase activity. PCR analysis of the <em>nifH</em> and <em>nodC</em> genes showed that the isolates possessed the <em>nifH</em> gene as a nitrogen fixation marker and the <em>nodC</em> gene as a nodulation marker. The findings show that, out of the 22 bacterial endophyte isolates, NTT1 and BOSH9 were the most effective in increasing plant height by 26.74% and 31.78%, respectively. Additionally, they resulted in an increase in biomass of 94.24% to 120.48% and an increase of 71.59% to 76.70% in grain yield compared to control treatment.</p> <p><strong>Conclusion:</strong> The selected isolates significantly enhance plant growth, increase biomass, and improve soybean grain yield. However, their potential use as biofertilizers in agriculture will require further investigation under real field conditions.</p> Marcelin Bahdjolbe , Alain-Martial Sontsa-Donhoung, Hawaou Abdouraman , Abel Wade, Richard Tobolbaï , Simon Thierry Okiobe, Dieudonne Nwaga Copyright (c) 2023 Bahdjolbe et al.; This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://journalmrji.com/index.php/MRJI/article/view/1417 Sun, 31 Dec 2023 00:00:00 +0000 Comparative Antimicrobial Activity of Lactobacillus acidophilus and Lactobacillus rhamnosus on Clinically Isolated Escherichia coli: An In vitro Study https://journalmrji.com/index.php/MRJI/article/view/1418 <p><strong>Aim: </strong>The aim of present study is to evaluate the comparative antimicrobial activity of <em>Lactobacillus acidophilus</em> and <em>Lactobacillus rhamnosus</em> on clinical isolates of <em>Escherichia coli.</em></p> <p><strong>Study Design:</strong>&nbsp; Cross-sectional observational study.</p> <p><strong>Place and Duration of Study: </strong>This study was conducted in Department of Medical Microbiology, School of Medical Education between January 2023 to September 2023.</p> <p><strong>Methodology: </strong>A total of 100 <em>E. coli</em> isolates collected from various diagnostic laboratories were included in the sample population and the prevalence of XDR, MDR, and non MDR isolates among them were determined by Kirby-Bauer disk diffusion method. The inhibitory activity of untreated and treated (pH adjusted) suspension of standard strains of <em>L. acidophilus </em>and <em>L. rhamnosus</em> on <em>E. coli</em> were determined by agar overlay method and the data was statistically analysed using ANOVA single-factor.</p> <p><strong>Results:</strong> The antimicrobial activity was confirmed for untreated and treated suspension of <em>Lactobacillus spp.</em> by measuring the zone of inhibition surrounding <em>E. coli</em> strains spotted on MRS medium but treated suspension exerted greater inhibitory activity than untreated suspensions of both <em>Lactobacillus spp</em>. and among the treated suspension <em>L. rhamnosus</em> exhibit greater inhibitory activity. Statistical analysis of this data using ANOVA single-factor was found to be not significant (<em>P &gt;0.05</em>), that is treated suspension of both <em>L. acidophilus</em> and <em>L. rhamnosus</em> has the independent activity against <em>E. coli. </em>While untreated suspensions of both <em>L. acidophilus</em> and <em>L. rhamnosus</em> was significant (<em>P =0.03</em>), that is untreated suspension of <em>L. rhamnosus</em> (mean inhibition of 12.19 mm) has greater inhibitory activity than untreated cell cultures of <em>L. acidophilus (</em>mean inhibition of 11.39 mm)<em>.</em></p> <p><strong>Conclusion: </strong>This result, disclosed that even if both <em>Lactobacillus spp.</em> exhibit antimicrobial activity against <em>E. coli</em>, <em>L. rhamnosus</em> showed greater inhibition than <em>L. acidophilus</em>. The study suggested the use probiotic <em>Lactobacillus</em> as a biotherapeutic in antibiotic resistant <em>E. coli</em> infection and should be further studied for their human health benefits.</p> Nithyamol Kuriakose , Ananthu Raj , Anju Anil, Azhar M M, Sajmi Sathar , Anagha S, Harish Kumar K S Copyright (c) 2023 Kuriakose et al.; This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://journalmrji.com/index.php/MRJI/article/view/1418 Sun, 31 Dec 2023 00:00:00 +0000 Identifying Sustainable Biomedical Solid Waste Management Practices in the Context of Limited Resources in Selected Healthcare Facilities in Douala Cameroon https://journalmrji.com/index.php/MRJI/article/view/1419 <p><strong>Aims: </strong>To Identify sustainable Biomedical solid waste management practices in the context of limited resources in selected HCFs in Douala, Cameroon.</p> <p><strong>Study Design:</strong>&nbsp; A cross-sectional survey with quantitative and qualitative study design was adopted for data collection from workers in the HCFs and also identifying the process of waste management.</p> <p><strong>Place and Duration of Study:</strong> This study was carried out in the some selected HCFs in the Douala region of Cameroon between January 2023 to June 2023.</p> <p><strong>Methodology: </strong>Study respondents were working in different sections of the HCFs and comprised of the following categories: doctors, laboratory scientists, cleaners, nurses and administrative/clerical workers. Field visitations and interactions with HCFs officials took place during the random sampling technique which was used in determining the sample size of 200 workers, Out of the sample size of 200 who were given questionnaires in order to provide data for the study, 150 valid mail questionnaires were retrieved and used for analysis. Every questionnaire was verified by validity. There’s also questions related funds, facilities availability, training and personal protective equipment. All of the data were coded and analyzed. The results were calculated based on sums and percentages of variables with SPSS vers.26 software and chi-square analysis with the level significance of p-value less than 0.05 was analyze</p> <p><strong>Results:</strong> This finding could also indicate that imbibing sustainable biomedical wastes management practices increases with higher experience in the hospital. Furthermore, communal bin disposal of biomedical wastes was the only statistically significant category under disposal method, indicating that waste disposal may be lacking in terms of sustainable practices. [30] also identified biomedical wastes disposal in open dumps among health workers in Saudi Arabia, attributable to poor sustainable waste training and lack of regulatory frameworks. I PCA results indicate that there are two dimensions of prevalent biomedical wastes in the hospital studied, as explicated by the two components retained</p> <p><strong>Conclusion:</strong> The limited resources and government policies and local councils influences sustainable waste management practices and this paper aimed at identifying some of the solutions towards improving Sustainable waste management policies. The practice of biomedical waste management in the study area was low. Level of education, taking training on biomedical waste management, availability of color-coded three bins, and attitude of health care workers were significantly associated with biomedical waste management practices. Hence, in-service training is recommended to improve biomedical waste management practices.</p> Nkwenti Peter, Etame Loe Gisele Marie , Nnanga Nga Copyright (c) 2023 Peter et al.; This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://journalmrji.com/index.php/MRJI/article/view/1419 Sun, 31 Dec 2023 00:00:00 +0000 Molecular Epidemiology of Fluoroquinolones Resistance Genes among Non-typhoidal Salmonella Species from Humans and Poultry Faeces in Ido-Ekiti, Ekiti State https://journalmrji.com/index.php/MRJI/article/view/1420 <p>Non-typhoidal <em>Salmonella</em> (NTS) strains are among the most common bacterial pathogens associated with antimicrobial resistance (AMR) where animals are known to be the major reservoir of NTS despite improvements in hygiene and sanitation. This study aimed to investigate the prevalence and basis of fluoroquinolone resistance genes in non-typhoidal <em>Salmonella</em> (NTS) recovered from human and poultry fecal samples in Ido Ekiti. The study used a total of 300 fecal samples,100 from humans and 200 from poultry sources. Faecal samples were cultured using Selenite F broth and XLD agar. Isolates were identified using morphology, biochemical tests, and molecular characterization. Antibiotic susceptibility were determined using disc diffusion test. Plasmid extraction and curing were done using alkaline lysis and Ethidium Bromide. Phylogenetic relatedness was determined using the Unweighted Pair Group Method with Arithmetic Mean. The results revealed that 10.7% of NTS were identified, with 9% from human and 11.5% from poultry sources. High resistance to ofloxacin (77.8%) was observed in both human and poultry samples. S. enteritidis from human sources had a concomitant presence of two qnr genes (qnrA and qnrS) at 20%, while only qnrS (60%) was detected from poultry sources. Statistically significant risk factors for NTS infection among human included the consumption of poultry products and the use of untreated poultry droppings. The study identified Qnr resistance gene in ESBL producing S. enteritidis from both human and poultry sources in Ido Ekiti and are plasmid borne, establishing the role of plasmids in the acquisition, and spread of antibiotic resistance of fluoroquinolones.</p> J. O. Ipinlaye , Oluyege A. O. Copyright (c) 2023 Ipinlaye and Oluyege; This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://journalmrji.com/index.php/MRJI/article/view/1420 Sun, 31 Dec 2023 00:00:00 +0000