Influence of Some Antibiotics and Essential Oils Used Alone or in Combination on the Vitality of Presumptive Probiotic Lactic acid Bacteria
Microbiology Research Journal International,
Aims: The aim of this study was to assess the in vitro antibacterial activity of selected antibiotics and essential oils alone or in combination, on selected presumptive probiotic lactic acid bacteria.
Study Design: Experimental studies.
Place and Duration of Study: Department of Microbiology of the University of Yaounde I between August 2017 and December 2017 (5 months).
Methodology: The chemical composition of five essential oils was determined by gas chromatography coupled with Solid-phase micro extraction. Then the sensitivity of four lactic acid bacteria to the essential oils and four antibiotics was assessed by the well diffusion and macrodilution method. Subsequently, two essential oils active on these bacteria and broad spectrum antibiotics were combined according to the central composite design plan.
Results: In general, the chemical composition of essential oils is very diverse, with the example of carvacrol found only in Origanum compactum at 53.24% and thymol in Thymus vulgaris at 56.19% and in Origanum compactum at 15.28%. The antibacterial activity shows that the majority of antibiotics used are active on the bacteria in the study compared to the essential oils where two were active (Origanum compactum and Cymbopogon winterianus). The evaluation of the combinations of essential oils and antibiotics in terms of kinetics has given us three cases: the first case is the one with no acidity or no growth at all; the second is the one where growth is normal; the third where growth is delayed with a more pronounced latency phase.
Conclusion: This study suggest that the effect of essential oils and medicinal plant used alone or in combination to antibiotics on the gut microbiota have to be evaluated for validation as well as their toxicity activities before using them for human therapy.
- Essential oils
How to Cite
Martinez J. The role of natural environments in the evolution of resistance traits in pathogenic bacteria. Proceedings of The Royal Society Biological sciences. 2009;276:2521–2530.
Neish A. Microbes in gastrointestinal health and disease. Gastroenterology. 2009;136:65-80.
Hooper L, Macpherson A. Immune adaptations that maintain homeostasis with the intestinal microbiota. Nature Reviews Immunology. 2010;10:159-169.
Qin J, Li R, Raes J, Arumugam M, Burgdorf K, Manichanh C et al. A human gut microbial gene catalogue established by metagenomic sequencing. Nature. 2010;464:59-65.
FAO/OMS. Health and nutritional properties of probiotics in food including. Cordoba, Argentina, 2001.
Rofes C. 2014 intérêts du microbiote intestinal et probiotique thèse. 2014;79:1-13
Francino M. Antibiotics and the human gut microbiome: Dysbioses and accumulation of resistances. Frontier microbiology. 2016;6:1-11.
Lange K, Buerger M, Stallmach A, Bruns T. Effets of antibiotics on gut microbiota: Review article PubMed. 2016;34:260-268.
Perry N, Perry E. Aromatherapy in the management of psychiatric dissorders clinical and neuropharmacological perspectives. CNS Drugs. 2006;20:257-80.
Maeda K, Ito T, Shioda S. Medical aromatherapy practice in japun. Essence 2012;10:14-6.
Adams R. Identification of essential oil components by gas chromatography/mass spectrometry, 4th ed.; 2012.
CLSI: Clinical and Laboratory Standards Institute. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard, 9 ed., 2007;32:1-68.
AOAC. Official methods of analysis, 13th ed. Washington DC, Association of official Analytical chemists; 1980.
Refrai M. Analyse microbiologique, étude de FOA alimentation 14.04 ; Organisation des nations unies pour alimentation et agriculture. 1981;202. French.
Hoswer D, Lemeshow S. Applied logistic regression. Book review. Statistics in medicine. 1989;10(7):1162-1163.
El Ghadraoui L, Essakhi D, Benjelloun M, Errabhi N, El HarchliEl H, Alaoui M et al. Chemical composition of essential oils from Rosmarinus officinalis L and Acridicide activity on Dociostaurus maroccanus Thunberg, 1815 in Morocco International. Journal of Scientific and Engineering Research. 2015;(6)8:166-172.
Chritiane T, Felipe M, Ana C, Ricardo J, Luis P, Eduardo A et al. Chemical composition of Rosmarinus officinalis essential oil and antioxidant action against gastric damage induced by absolute ethanol in the rat. Asian Pacific journal of Tropical Biomedecine. 2016;6(8): 677-681.
Bouhdid S, Idaomar M, Zhiri A, Baudoux D, Senhajiskli N, Abrini J. L’effet antibactérien in vitro de l’huile essentielle d’Origanum compactum vis à vis de souches d’origine clinique. Nouvelles Tendances dans l’Ingénierie Biomédicale. 2005;11:142-9. French.
Boruga O, Jianu C, Misca C, Colet I, Gruva A, Horhat F. Thymus Vulgaris essential oil: Chemical composition and antimicrobial activity. Journal of Medicine and Life. 2014;7(3):56-60.
Marciel M, Morais S, Bevilaqua C, Silva R, Barros R, Sousa R, et al. Chemical composition of Eucalyptus spp. essential oils and their insecticidal effects on lutzomyia longipal pis. Veterinary Parasitology. 2010;167:1-7.
Madouri L, Asma B, Madari K, Bev-ould Z, Rigou R. Chemical composition, antibacterial and antioxidant activities of essential oil of Eucalytus Globulus from Algeria. Industrial Crops and Products. 2015;78:148-153.
Chebli B, Mohamed A, Idrissi L, Hmamouchi M. Chemical composition and antifungal activity of oils of seven Moroccan labiatae against botrytis cinerea. Journal of Ethnopharmacology. 2003;89:165-169.
Bouhdid S, Skali S, Idaomar M, Zhiri A, Baudoux D, Amensour M et al. Antibacterial and antioxidant activities of Origanum compactum essential oil. African Journal of Biotechnology. 2008;10:1563-70.
Simic A, Rancic A, Sokovic M, Ristic M, Grujic-Jovanovic S, Vukojevic J. Essential oil composition of Cymbopogon winterianus and Carum carvi and their antimicrobial activities. Pharmaceutical Biology. 2008;46(6):437-441.
Kuete V. Potential of Cameroonian plants and derived product against microbial infections: A Review Planta Medica. 2010;76(14):1479-1491.
Dawang N, Datup A. Screening of five medicinal plants for treatment of typhoid fever and gastroenteritis in Central Nigeria. Global Engineers and Technologists Review. 2012;2:1–5.
Takeo O, Masato K, Keiko S, Rika O, Junko M, Hiroshi I et al. In vitro and in vivo antimicrobial activities of tricyclic ketolide Te-802 and its analogs. Journal of Antibiotics. 2004;57:518-527.
Dorman HJ, Deans SG. Antimicrobial agents from plants: Antibacterial activity of plant volatile oils. Journal of Applied Microbiology. 2000;88(2):308-316
Ultee A, Bennik M, Moezelaar R. The phenolic hydroxyl group of carvacrol is essential for action against the food-born pathogen Bacillus cereus. Applied and Environmental Microbiology. 2002;4:1561-1568.
Cowan M. Plants products as antimicrobial agents. Clinique Microbiology Review. 1999;12:564–584.
Sanogo R, Maiga A, Daillo D. Activités analgésique et antiinflammatoire des extraits de Maytenius senegalensis, stereospermum kuntrianum et tricilia emetica utilisées dans le traitement traditionnel des dysménorrhées au Mali. Pharmacopée et Médecine Traditionnelle Africaine. 2006;14:123-136. French.
Bouyahya A, Jamal A, Edaoudi F, Et-Touys A, Bakri Y, Dakka N. Origanum compactum Benth: A review on phytochemistry and pharmacological properties. Medicinal and Aromatic Plants. 2016;5(4):252.
Sharifi-Rad M, Varoni EM, Iriti M, Martorell M, Setzer WN, Contrera M del Mar et al. Carvacrol and human health: A comprehensive review. Phytotherapy Research. 2018;1-13.
Bouyahya A, Zengin G, Belmehdi O, Bourais I, Chamkhi I, Taha D. Origanum compactum Benth., from traditional use to biotechnological applications. Journal of Food Biochemistry. 2020;1-24.
Mondal A, Bose S, Mazumder K, Khanra R. Carvacrol (Origanum vulgare): Therapeutic properties and molecular mechanisms. Bioactive Natural Products for Pharmaceutical Applications. 2020;437-462.
Wany A, Jha S, Nigam VK, Pandey M. Chemical analysis and therapeutic uses of citronella oil from Cymbopogon winterianus: A short review. International Journal of Advanced Research. 2013;1(6):504-521.
Avoseh O, Oyedeji O, Rungqu P, Nkeh-Chungag B, Oyedeji A. Cymbopogon species; ethnopharmacology, phytochemistry and the pharmacological importance. Molecules. 2015;20:7438-7453.
Sharma R, Rao R, Kumar S, Mahant S, Khatkar S. Therapeutic potential of citronella essential oil: A review. Current Drug Discovery Technologies. 2019;16(4):330-339.
Bayala B, Coulibaly AY, Djigma FW, Nagalo BM, Baron S, Figueredo G et al. Chemical composition, antioxidant, anti-inflammatory and antiproliferative activities of the essential oil of Cymbopogon nardus, a plant used in traditional medicine. Bio Mol Concepts. 2020;11:86-96.
Bollenbach T. Antimicrobial interactions: mechanisms and implications for drug discovery and resistance evolution. Current Opinion in Microbiology. 2015;27 :1–9.
Singh S, Barrett J. Empirical antibacterial drug discovery - foundation in natural products. Biochemistry of Pharmacology. 2006;71:1006-1015.
Cox S, Mann C, Markham J, Bell H, Gustafson J, Warmington. The mode of antimicrobial action of the essential oil of melaleuca alternative (tea tree oil). Journal of Applied Microbiology. 2000;88:170- 175.
Prescott L, Harley J, Klein D. La chimiothérapie antimicrobienne: Microbiologie. 2ème édition, Bruxelles. 2003;806–811. French.
Burt S. Essential oils: their antibacterial properties and potential applications in foods a review. International Journal of. Food Microbiology. 2004;94:223-253.
Lambert R, Skandamis P, Coote P, Nychas G. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. Journal of Applied of Microbiology. 2001;91:453-462.
Bakkali, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils. Food and Chemical Toxicology. 2008;46:446-475.
Akbli M, Rhallabi N, Ait Mhana R, Akssira M, and Mellouki F. Activité antibacterienne de l’huile essentielle de la sciure du bois de loupe de Tetraclinie articulata. International Journal of Innovation and Applied Studies. 2016;16(2):314-321. French.
Bouyahya A, Bakri Y, Et-Touys A, Talbaoui A, Charfi S, Abrini J et al. Resistance of essential oils against bacteria . Lavoisier Phytotherapie. 2016;1-10.
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