Presence and Virulence Potential of Aeromonas hydrophila in Selected Water Sources for Household Consumption in Makurdi, Benue State
Microbiology Research Journal International,
This study was conducted to investigate the prevalence of Aeromonas hydrophila in selected drinking water sources in Makurdi. A total of 100 water samples (Tap, river, stream, well, pond and borehole water) were collected from different locations in Makurdi. Isolation and identification of the organism was performed using standard microbiological techniques. Further confirmation of the isolates as Aeromonas hydrophila was carried out using the Microbact 24E detection kit and polymerase chain reaction (PCR). A. hydrophila was detected in 12 (12%) out of the 100 samples; 6.67%, 8.82%, 7.14%, 25%, 30% and 20% of tap, well, borehole, river, pond and stream water samples respectively. The highest isolation rate of A. hydrophila (30%) was from pond water. All A. hydrophila isolated exhibited heamolysin, protease and lipase activity. The findings of this study revealed that treated and untreated drinking water sources in Makurdi are contaminated with potentially virulent A. hydrophila strains which may pose a health risk to consumers. Therefore, basic water treatment should be applied to drinking water to reduce public health threat posed by this finding.
- Aeromonas hydrophila
- drinking water
- virulence factors
How to Cite
Stratev D, Odeyemi OA. Antimicrobial resistance of Aeromonas hydrophila isolated from different food sources: A mini-review. Journal of Infection and Public Health. 2016;9:535-544.
Park SH, Choi S, Ha S. Predictive modeling for the growth of Aeromonas hydrophila on lettuce as a function of combined storage temperature and relative humidity. Food Borne Pathogens and Disease. 2019;20(20):1-8
Abd-El-Malek AM. Incidence and virulence characteristics of Aeromonas spp. in fish. Veterinary World. 2017;10(1):34-37.
Yano Y, Hamano K, Tsutsui I, Aue-umneoy D, Ban M. Occurrence, molecular characterization, and antimicrobial susceptibility of Aeromonas spp. in marine species of shrimps cultured at inland low salinity ponds. Food Microbiology. 2015;47:21-27.
Poffe R, Op de Beeck E. Enumeration of Aeromonas hydrophila from domestic wastewater treatment plants and surface waters. Journal of Applied Bacteriology. 1991;71(366-370).
Ansari M, Rahimi E, Raissy M. Antibiotic susceptibility and resistance of Aeromonas spp isolated from fish. African Journal of Microbiology Research. 2011;5(31):5772 – 5775.
Fadel HM, El-lamine MMM. Vibriosis and Aeromonas infection in shrimp: Isolation, sequencing, and control. International Journal of One Health. 2019;5:38-48.
Igbinosa IH, Igumbor EU, Aghdasi F, Tan M, Okoh AI. Emerging Aeromonas species infections and their significance in public health. The scientific World Journal. 2102;1-13.
Adegoke CO, Ogunbanwo ST. Isolation and characterisation of Aeromonas species isolated from food and Diarrhoeagenic stool in Ibadan metropolis, Nigeria. Food Science and Quality Management. 2016;51:20-31
Figueras Salvat MJ, Ashbolt N. Aeromonas. In: J.B. Rose and B. Jiménez-Cisneros, (eds) Global Water Pathogen Project; 2019.
Pandove G, Sahota PP, Achal V, Vikal Y. Detection of Aeromonas hydrophila in water using PCR. Journal of American Water Works Association. 2011;103(11) :59-65.
Krovacek K, Dumontet S, Eriksson E, Baloda SB. Isolation and Virulence profiles of Aeromonas hydrophila implicated in an outbreak of food poisoning in Sweden. Microbiology and Immunology. 1995; 39:655 – 661.
Zhang Q, Shi G, Tang G, Zou Z, Yao G, Zeng G. A foodborne outbreak of Aeromonas hydrophila in a college, Xingyi city, Guizhou, China. Western Pacific Surveillance and Response Journal. 2012;3(3):1-4
Bhowmik P, Bag PK, Hajra TK, Sarkar RP, Ramamurthy T. Pathogenic potential of Aeromonas hydrophila isolated from surface waters in Kolkata, India. Journal of Medical Microbiology. 2009;58:1549-1558.
Katz MJ, Parrish NM, Belani A, Shah M. Recurrent Aeromonas bacteremia due to contaminated well water. Open Forum Infectious Diseases. 2015;1-3.
Khor WC, Puah SM, Tan JAMA, Puthucheary SD, Chua KH. Phenotypic and genetic diversity of Aeromonas species isolated from fresh water lakes in Malaysia, PLoS ONE. 2015;10(12):1-13
Palumbo SA, Moragan DR, Buchanan RL. Influence of temperature, NaCl, and pH on the growth of Aeromonas hydrophila. Journal of Food Science. 1985;50 (5):1417–1421.
Harrigan WF, McCance ME. Laboratory methods in food and dairy microbiology. Academic press; 1976.
Cowan ST. Cowan and steel’s manual for the identification of medical bacteria, 3rd edition, Cambridge University Press; 1986.
Holt JG. The shorter bergey’s manual of determinative bacteriology.8th Edition. The Williams and Wilkins Company, Baltimore USA; 1981.
Hussain IA, Jeyasekaran G, JeyaShakila R, Raj KT, Jeevithan E. Detection of hemolytic strains of Aeromonas hydrophila and A. sobria along with other Aeromonas spp. from fish and fishery products by multiplex PCR. Journal of Food Science and Technology. 2014;51(2):401–407.
Wang G, Clark CG, Liu C, Pucknell C, Munro CK, Kruk TMAC, Caldeira R. Woodward DL. F.G. Rodgers, Detection and characterization of the hemolysin genes in Aeromonas hydrophila and Aeromonas sobria by multiplex PCR. Journal of Clinical Microbiology. 2003;41 :1048–1054.
Corry JEL, Curtis GDW, Baird RM. (Eds.) Starch Ampicillin Agar (SAA). Pharmacopeia of culture media. In: Handbook of culture media for food microbiology, 2nd edition. Progress in Industrial Microbiology. 2003;37:600-602.
Persson S, Al-Shuweli S, Yapici S, Jensen JN, Olsen KEP. Identification of clinical Aeromonas species by rpoB and gyrB sequencing and development of a multiplex PCR method for detection of Aeromonas hydrophila, A. caviae, A. veronii, and A. media. Journal of Clinical Microbiology. 2015;53(2):653- 656.
Cagatay IT, Sen EB, Detection of pathogenic Aeromonas hydrophila from rainbow trout (Oncorhynchus mykiss) farms in Turkey. International Journal of Agriculture and Biology. 2014;16:435-438.
Casco’n A, Anguita J, Hernanz C, Sa’nchez M, Ferna’ndez M, Naharro G. Identification of Aeromonas hydrophila hybridization group 1 by PCR assays. Applied and Environmental Microbiology. 1996;62(4):1167-1170.
Sarkar A, Saha M, Roy P. Identification and Typing of Aeromonas hydrophila through 16S rDNA-PCR Fingerprinting. J. Aqua. Res. Dev. 2012;3(6):1-4
Othman RM, Al-Thahe FS, Faaz1 RA and Jassim HY. Molecular detection of Aeromonas hydrophila isolated from infected carp Cyprinus carpio breeding in aquafarming in Basra, Iraq, Life Science Archives, 2017;3(2):974 – 980.
Fuzihara TO, Pisani B, Simoes M, Brigido BM, Leopoldoeslva C, Vannucci L, Arioshi K. The occurrence of Aeromonas spp in drinking water. Rev Inst Adolfo Lutz. 2005;64(1):122-7.
Kivanc M, Yilmaz M, Demir F. The occurence of Aeromonas in drinking water, Tap water and porsuk river. Brazilian Journal of Microbiology. 2011;42:126-131
Zaky MM, Salem M, Persson KM, Eslamian S. Incidence of Aeromonas species isolated from water and fish sources from Lake Manzala (Egypt). International Journal of Hydrology Science and Technology. 2011;1(3):312-414.
Bello HS, Mustapha A, Ismail HY, Isa MA, Mangga HK, Detection of Aeromonas species from water sources in North Eastern Nigeria. International Journal of innovative Science, Engineering and Technology. 2016;3(2):140-148.
Senderovich Y, Ken-Dror S, Vainblat I, Blau D, Izhaki I, Halpern M. A molecular study on the prevalence and virulence potential of Aeromonas spp. recovered from patients suffering from diarrhea in Israel; 2012.
Koksal F, Oguzkurt N, Samasti M, Atlas K. Prevalence and antimicrobial resistance patterns of Aeromonas strains isolated from drinking water samples in Istanbul, Turkey. Chemotherapy. 2007;53:30-35
Scoaris DO, Bizerra FC, Yamada-ogatta SF, Filho BAA, Ueda-Nakamura T, Nakaramua CV, Filho BPD. The occurrence of Aeromonas spp. In the bottled mineral water, well water and tap water from the Municipal supplies. Brazilian Archives of Biology and Technology. 2008;51(5):1049-1055.
Villarruel-Lo´ pez EA, Ferna´ndez-Rendo´n L, Mota-de-la-Garza J, Ortigoza-Ferado. Presence of Aeromonas spp in water from drinking-water- and wastewater-treatment plants in Me´xico city. Water Environment Research. 2005;77( 7):3074- 3079.
Rasmussen-Ivey CR, Figueras MJ, McGarey D, Liles MR. Virulence Factors of Aeromonas hydrophila :In the wake of Reclassification. Frontiers in Microbiology. 2016;7(1337):1-10.
Stratev D, Vashin I, Rusev V. Prevalence and survival of Aeromonas spp.in foods – A review. Revue Méd. Vét. 2012;163 (10):486-494
Pablos M, Remacha MA, Rodriguez-Calleja JM, Santos JA, Otero A, Garcia-Lopez ML. Identity, virulence genes, and clonal relatedness of Aeromonas isolates from patients with diarrhea and drinking water. European Journal of Clinical Microbiology and Infectious Diseases. 2010;29:1163-1172.
Abstract View: 87 times
PDF Download: 54 times