Bacteriological Profile of Campylobacter SPP Isolated from Pigs in Cameroon: A Review
Microbiology Research Journal International,
Campylobacter is the most frequent bacteria implicated in acute gastroenteritis in the industrialized world and is considered as a major public health problem. The aim of this review is to improve our knowledge on the bacteriological profile of Campylobacter isolated from pigs. Porks, beef also represent sources of infection with these microorganisms. Campylobacter is a bacterium comprising seventeen species, including Campylobacter jejuni, Campylobacter coli and Campylobacter fetus. C. coli is the most common Campylobacter species recovered from pigs. The prevalence (46%, 52%, 75%) varies from one country to another, from the collection site. C. jejuni and C. coli grows best in a low oxygen or microaerophilic environment. The virulence markers varies among different sources of the isolates. The majority of genes were found at high levels in Campylobacter spp. isolated from pork meat (csrA, sodB, cdtB, and racR). Moreover, this review revealed virulent properties of Campylobacter isolated from swine products and high resistance rates to Tetracycline (68%), Erythromycin (61%), which may represent difficulties in campylobacteriosis treatment. Fluoroquinolones resistance (2.2%, 7%, 11%,100%) varies according to the country and the source of the sample. It will be wise to insist on hygenic measures as a solution to limit the incidence of Campylobacter and prevent dissemination of pathogens in animals (chicken, pork and beef) within slaughter-houses/ killings Cameroon.
How to Cite
Fosse J, Laroche M, Rossero A, Seeger H, Magras C et al. Suivi de la contamina tion de lots de porcs par Staphylococcus aureus et Yersinia enterocolitica de l’élevage à l’abattoir: une étude explora trice, Revue Méd. Vét. 2010;161(1):20–29
European Food Safety Authority (EFSA), European Centre for Disease Prevention and Control (ECDC). The European union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2015. EFSA J. 2016;14: 04634.
Karesh WB, Dobson A, Lloyd Smith JO, Lubroth J, Dixon MA, Bennett M, et al. Ecology of zoonoses: Natural and unnatu ral histories. Lancet. 2012;380:1936–1945.
Wilson DJ, Gabriel E, Leatherbarrow AJH, Cheesbrough J, Gee S, Bolton E, et al. Tracing the source of campylobacteriosis. PLoS Genet. 2008;4:1–9.
Sheppard SK, Dallas JF, Strachan NJC, Mac Rae M, Mc Carthy ND, Wilson DJ, et al. Campylobacter genotyping to determine the source of human infection. Clin. Infect. Dis. 2009;48:1072–1078.
European Food Safety Authority. The community summary report on trends and sources of zoonoses, zoonotic agents, antimicrobial resistance and foodborne outbreaks in the European Union in 2005. EFSA J. 2006;94:4–288.
Altekruse SF, Stern NJ, Fields PI, Swerdlow DL. Campylobacter jejuni—An emerging foodbourne pathogen. Emerg. Infect. Dis. 1999;5:28–35.
Nguyen, H. Acanthamoeba-campylobacter interactions; University of Ottawa: Ottowa, ON, Canada; 2001.
Australian national notifiable diseases surveillance system; number of notifica tions for all diseases by year, Australia, 1991 to 2009 and Year-to-Date Notifi cations for; Australian Department of Health and Aging: Adelaide, Australia; 2010.
Gharst G, Oyarzabal OA, Hussain SK. Review of current methodologies to isolate and identify Campylobacter spp. from foods. J. Microbiol. Methods 2013;95:84–92.
Padungton P, Kaneene JB. Campylobacter spp. in humans, chickens, pigs and their antimicrobial resistance. J. Vet. Med. Sci. 2003;65:161–170.
Adams MR, Moss MO. Food microbiology; The Royal Society of Chemistry: Cambridge, UK; 2008.
EFSA. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2017. EFSA J. 2018;16:5500.
Kashoma IP, Kassem II, John J, Kessy BM, Gebreyes W, Kazwala RR, et al. Prevalence and antimicrobial resistance of campylobacter isolated from dressed beef carcasses and raw milk in Tanzania. Microb. Drug. Resist. 2016;22:40–52.
Wieczorek K, Osek J. Antimicrobial resi stance and genotypes of Campylobacter jejuni from pig and cattle carcasses isolated in Poland during 2009–2016. Microb. Drug Resist. 2018;24:680–684.
Wysok B, Wojtacka J. Detection of virulence genes determining the ability to adhere and invade in campylobacter spp. from cattle and swine in Poland. Microb. Pathog. 2018;115:257–263.
Korsak D, Ma´ckiw E, Ro˙zynek E, Zylowska M. Prevalence of campylobacter spp. in retail chicken, Turkey, Pork, and beef meat in Poland between 2009 and 2013. J. Food Prot. 2018;78:1024–1028.
Narvaez Bravo C, Taboada EN, Mutschall SK, Aslam M. Epidemiology of antimicro bial resistant Campylobacter spp. isolated from retail meats in Canada. Int. J. Food Microbiol. 2017;17:43–47.
EFSA. The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2017. EFSA J. 2019;17:5598.
Park SF. The physiology of Campylobacter species and its relevance to their role as foodborne pathogens. Int J Food Microbiol. 2000;74:177–188.
Dasti JI, Tareen AM, Lugert R, Zautner AE, Gross U. Campylobacter jejuni: A brief overview on pathogenicity-associated fac tors and disease-mediating mechanisms. Int. J. Med. Microbiol. 2010;300: 205–211.
Carvalho AC, Ruiz Palacios GM, Ramos Cervantes P, Cervantes LE, Jiang X, Pickering LK. Molecular characterization of invasive and noninvasive Campylobacter jejuni and Campylobacter coli isolates. J. Clin. Microbiol. 2001;39:1353–1359.
Hickey TE, Mcveigh AL, Scott DA, Michielutti RE, Bixby A, Carroll SA et al. Campylobacter jejuni Cytolethal distending toxin mediates release of interleukin-8 from intestinal epithelial cells. Infect. Immun. 2000;68:6535–6541.
Tresse O, Alvarez Ordonez A, Connerton IF. Editorial: About the Food borne Patho gen Campylobacter. Front Microbiol. 2017; 8:1908.
Penner JL. The genus Campylobacter: A decade of progress. Clin Microbiol Rev 1988;1:157-72
Ketley JM. Pathogenesis of enteric infection by Campylobacter. Microbiology 1997; 143:5-21.
Nachamkin I. Campylobacter and Arcobacter. In: Manual of clinical micro biology. 6th ed. Washington: ASM Press. 1995;483-91.
Hana Trigui, Valeria E Paquet, Steve J Charrette and Sébatien P. Faucher. Packaging of campylobacter jejuni into multilamellar bodies by the ciliate 2 tetrahy mena pyriformis. Appl. Environ. Microbiol ; 2016. DOI:10.1128/AEM.03921-15.
Alter T, Gaull F, Kasimir S, Gurtler M, Mielke H, Linnebur M, et al. Prevalences and transmission routs of Campylobacter spp. strains within multiple pig farms. Vet Microbiol. 2005;108:251–61.
Jensen AN, Dalsgaard A, Baggesen DL, Nielsen EM. The occurrence and characterization of Campylobacter jejuni and C. coli in organic pigs and their outdoor environment. Vet Microbiol. 2006;116:96–105.
Young CR, Harvey R, Anderson R, Nisbet D, Stanker LH. Enteric colonization following natural exposure to Campylo bacter in pigs. Res Vet Sci. 2000;68:75–8.
Gundogdu O, Bently SD, Holden MT, Parkhill J, Dorrell N, Wren BW. Reanno tation and re-analysis of the Campy lobacter jejuni NCTC11168 genome sequence. BMC Genomics 2007;8:471–472.
Altekruse SF, Stern NJ, Fields PI, Swerdlow DL. Campylobacter jejuni and emerging foodborne pathogen. Emerg. Infect. Dis. 1999;5:28–35.
Coker O, Akitoye I, Raphael D, Thomas N, Bolaji A, Kehinde O, et al. Human camp ylobacteriosis in developing countries. Emerg. Infect. Dis. 2002 ;8:237–243.
Laprade N, Cloutier M, Lapen DR, Topp E, Wilkes G, Villemur R, et al. Detection of virulence, antibiotic resistance and toxin (VAT) genes in Campylobacter species using newly developed multiplex PCR assays. J Microbiol. Methods. 2016;124: 41–47.
Klanˇcnik A, Vuˇckovi´c D, Jamnik P, Abram M, Možina SS. Stress Response and Virulence of Heat Stressed Campylo bacter jejuni. Microbes Environ. 2014; 29:338–345.
Malgorzata Andrzejewska, Bernadeta Szczepa ´nska, Dorota Spica and Jacek J Klawe. Prevalence, virulence and antimicro bial resistance of campylobacter spp. in raw milk, beef and porkmeat in Northern Poland. Foods. 2019;8:420. DOI:10.3390/foods8090420
Harvey RB, Young CR, Ziprin RL, Hume ME, Genovese KJ, Anderson RC, et al. Prevalence of campylobacter spp isolated from the intestinal tract of pigs raised in an integrated swine production system. J Am Vet Med Assoc. 1999;215:16014.
Taylor DJ. Miscellaneous bacterial infec tions: Campylobacter. In: Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW, editors. Diseases of swine. 10th ed. Ames, IA: Wiley Blackwell. 2012;8724.
Pearce RA, Wallace FM, Call JE, Dudley RL, Oser A, Yoder L, et al. Prevalence of Campylobacter within a swine slaughter and processing facility. J Food Prot 2003;66:1550–6.
Malakauskas M, Jorgensen K, Nielsen EM, Ojeniyi B, Olsen JE. Isolation of campy lobacter spp. from a pig slaughterhouse and analysis of cross contamination. Int J Food Microbiol. 2006;108:295–300.
Niesen EM, Engberg J., Madsen M. Distribution of serotypes of Campylobacter jejuni and C.coli from Danish patients, poultry, cattle and swine. FEMS Immunol. Med.Microbiol. 1997;19:47-56.
Reina J, Munoz C, Serra A. Acta Gastro enterol belgi Suppl. 1993;56:22.
WHO. The Global View of Campylo bacteriosis; World Health Organization: Geneva, Switzerland, 2013;69.
Moore JE, Barton MD, Blair IS, Corcoran D, Dooley JS, Fanning S, et al. The epide miology of antibiotic resistance in Campylobacter. Microbes Infect. 2006 ;8:1955–1966.
Jonker A, Picard JA. Antimicrobial susceptibility in thermophilic Campylo bacter species isolated from pigs and chickens in South Africa. J. S. Afr. Vet. Assoc. 2010;81:228–236.
Ghunaim H, Behnke JM, Aigha I, Sharma A, Doiphode SH. Analysis of resistance to antimicrobials and presence of virulence/ stress response genes in Campylobacter isolates from patients with severe diarrhoea. PLoS ONE. 2015;10: 0119268.
Jonker A, Picard JA. Antimicrobial susceptibility in thermophilic Campylo bacter species isolated from pigs and chickens in South Africa. J S Afr Vet. Assoc. 2010;81:228–236.
Reddy S, Zishiri OT. Detection and prevalence of antimicrobial resistance genes in campylobacter spp. Isolated from chickens and humans. Onderstepoort J Vet Res. 2017;84:1–6.
Bolton, D.J. Campylobacter virulence and survival factors. Food Microbiol. 2016;48: 99–108.
Epps SVR, Harvey RB, Hume ME, Phillips TD, Anderson RC, Nisbet DJ. Foodborne campylobacter: Infections, metabolism, pathogenesis and reservoirs. Int. J. Environ. Res. Public Health. 2013;10: 6292–6304.
Kawai F, Paek S, Choi KJ, Prouty M, Kanipes MI, Guerry P, et al. Crystal structure of JlpA, a surface-exposed lipoprotein adhesin of Campylobacter jejuni. J. Struct. Biol. 2012;177:583–588.
Koolman L, Whyte P, Burgess C, Bolton D. Virulence gene expression, adhesion and invasion of Campylobacter jejuni exposed to oxidative stress (H2O). Int J Food. Microbiol. 2016;220:33–38.
Silva J, Leite D, Fernandes M, Mena C, Gibbs PA, Teixeira P. Campylobacter spp. as a Foodborne Pathogen: A Review. Front. Microbiol. 2011;2:200.
Rokosz Chudziak N, Rastawicki W. Wybrane mechanizmy chorobotwórczo´sci paleczek Campylobacter jejuni. Med. Dosw. Mikrobiol. 2014;66:47–58. (In Polish)
Karikari AB, Obiri Danso K, Frimpong EH, Krogfelt KA. Antibiotic resistance of campylobacter recovered from faeces and carcasses of healthy livestock. BioMed Res. 2017;4091856.
Kouassi Eugène Koffi, Kalpy Julien Coulibaly, Nguessan Daniel Saraka, Claire Brice Valéry Senin, Haussain boka et mireille dosso. Évaluation de la qualité microbiologique des carcasses de porcs abattus à la SIVAC: Recherche de Salmonella, Yersinia et Campylobacter. Afrique science. 2020;16(1):30–38. ISSN:1813-548X, Available :http://www.afriquescience.net
Denis M, Chidaine B, Laisney MJ, Kempf I, Rivoal K, Mégraud F, et al. Comparison of genetic proﬁles of campylobacter strains isolated from poultry, pig and campylo bacter human infections in Brittany, France. DOI:10.1016/j.patbio.2008.04.007
Jensen AN, Adalsgaard DL, Baggesen EM Nielsen. The occurrence and characteri zation of Campylobacter Jejuni and C.coli in organic pigs and their outdoor environ ment. Veterinary microbiology. 2006;116: 96-105. DOI : 10.1016/j.vetmic.2006.03.006
Daniel A Tadesse, Thomas Wittum, Peter B Bahnson, Fred DeGraves, Julie A Funk, Thakur Siddhartha, et al. Prevalence and antimicrobial resistance proﬁle of campylo bacter spp. Isolated from conventional and antimicrobial-free swine production systems from different U.S. Regions. Food borne pathogens and disease; 2011;8:3. DOI: 10.1089=fpd.2010.0665
Eric Burrough, Samantha Terhorst, Orhan Sahin, Qijing Zhang. Prevalence of campylobacter spp. relative to other enteric pathogens in grow-ﬁnish pigs with diarrhea. Available:http://dx.doi.org/10.1016/j.anaerobe.2013.06.004
Evelyne Guévremont, Éric Nadeau, Marc Sirois, Sylvain Quessy. Antimicrobial susce ptibilities of thermophilic Campylobacter from humans, swine and chicken broilers. The Canadian Journal of Veterinary Research. 2006;70:81–86.
Goualie GB, Essoh EA, Elise Solange KN, Natalie G, Souleymane B, Lamine Sebas tien N, et al. Prevalence and antimicrobial resistance of thermophilic campylobacter isolated from chicken in Cote d’Ivoire. Int J Microbiol. 2012;150612.
Humphrey T, O’Brien S, Madsen M. Campylobacters as zoonotic pathogens: Afood production perspective. Int J Food Microbiol. 2007;117:237–257.
Pieracci EG, Hall AJ, Gharpure R, Haile A, Walelign E, Deressa A et al. Prioritizing zoonotic diseases in Ethiopia using a one health approach. One Health. 2016;2:131–135.
Stern NJ, Robach MC. Enumeration of Campylobacter spp. in broiler feces and in corresponding processed carcasses. J Food Prot. 2003;66:1557–63.
Izat AL, Gardner FA, Denton JH, Golan FA. Incidence and level of Campylobacter jejuni in broiler processing. Poult Sci. 1988; 67:1568–72.
Abstract View: 281 times
PDF Download: 208 times