Sources of Fungal Contamination of Fresh and Dried Fish in Kisii County, Kenya

Main Article Content

Irene Seila Nyamwaka
Ethel Monda
Omwoyo Ombori
Johnson Kwach


Aflatoxins are toxic secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus that frequently invade foods and feedstuffs. In humid climatic conditions like those found in Kisii County, growth of fungi on dried fish and fish feeds is accelerated due to absorption of moisture from the environment as a result of poor storage and sometimes improper drying. This can lead to creation of favourable conditions for the growth of the toxigenic fungi and consequently production of aflatoxins. This study was conducted to determine the main sources of fungal contamination of selected fresh and dried fish sold in Kisii county, Kenya. Samples of fish were randomly obtained from four main markets in the county. A structured questionnaire was used to determine the sources of fish, mode and hygiene of transportation, handling of fish, duration of storage, hygiene of packaging materials and display environment of fish. Levels of fish contamination were expressed in form of colony forming units per gram (CFU/g) of fish. One-way analysis of variance was used to determine mean differences in parameters analysed. The results revealed that CFU/g of fish from control samples were significantly less than those from fresh and dried fish samples from the various sources (p<0.05). There was generally no significant difference between CFU/g from both fresh and dried fish from the various sources. The study found out that fresh and dried fish sold in Kisii county are contaminated with fungi. There is therefore need for frequent surveillance on the sources of fish contamination and public sensitization of all those involved in fish supply chain.  This will help reduce possible aflatoxin contamination thus promoting consumers’ healthy living.

Fresh fish, dried fish, contamination, CFU, sources, fungal

Article Details

How to Cite
Nyamwaka, I. S., Monda, E., Ombori, O., & Kwach, J. (2020). Sources of Fungal Contamination of Fresh and Dried Fish in Kisii County, Kenya. Microbiology Research Journal International, 30(10), 50-62.
Original Research Article


El-Ahl MHS. Studies on fungi in fish and fish products and their control. Ph.D. thesis, department of microbiology, faculty of veterinary medicine, Cairo University; 2010.

Hassan AA, Hammad AM, El Barawy AM, Manal AH. Incidence of aflatoxigenic fungi in frozen and canned fishes and trials to inhibit aflatoxin production by use of some minor elements and lupinustermis seeds. Egypt. Journal of Applied Sciences. 2007; 22(10B):351-360.

Adebayo-Tayo BC, Onilude AA, Bukola C, Abiodun A, Ukpe GP. Mycofloral of smoke-dried fishes sold in Uyo, Eastern Nigeria. World Journal of Agricultural Sciences. 2008;4(3): 346-350.

Siscovick DS, Radhunathan TE, King I, Weinmann S, Bovbjerg VE, Kushi L, Knopp RH. Dietary intake of long chain n-3 poly-unsaturated fatty acids and the risk of primary cardiac arrest. American Journal of Clinical Nutrition. 2000; 71:2085-212

Ghaly AE, Dave D, Budge S, Brooks MS. Fish spoilage mechanism and preservation techniques review. American Journal of Applied Sciences. 2010;7(7):859-877.

Adeyemo OK. Consequences of pollution and degradation of Nigerian aquatic environment on fisheries resources. The Environmentalist. 2003;23(4):297-306.

Nyamwaka IS, Nyamache AK, Maingi JM. Microfungi associated with sun dried Rastrineobola argentea sold in Gucha South District in Kenya. Microbiology Research Journal International. 2017; 19(3):1-8.

DFKC (Directorate of Fisheries Kisii County). Annual report for 2014. Kisii County, Kenya; 2015.

Wen Y, Hatabayash, H, Arai H, Kitamoto M, Yabe K. Function of the cypX and moxY genes in aflatoxin biosynthesis in Aspergillus parasiticus. Applied and Environmental Microbiology. 2004; 6:3192–3198.

Shephard GS. Risk assessment of aflatoxins in food in Africa. Food Additives and contaminants: Part A: Chemistry, analysis, control, exposure and risk assessment. 2008;25(10):1246–1256.

Zain ME. Impact of mycotoxins on humans and animals. Journal of Saudi Chemical Society. 2011;15:129–144.

Khan MA, Asghar A, Ahmed J, Iqbal T, Shamsuddin ZA. Reduction of aflatoxins in dundi-cut whole red chillies (Capsicum indicum) by manual sorting technique. Science and Technology Development Journal. 2013;32:16–23.

Jaetzold R, Schmidt H. Farm management handbook of Kenya. Vol II. Natural conditions and farm management information part A WEST KENYA (Nyanza and Western provinces). Kenya Ministry of Agriculture. 1983;245-285.

Climate Data. Climate Kisii. Weather by month, average temperature precipitation and humidity. org.; 2019. Accessed 23rd April, 2019.

KNBS (Kenya National Bureau of Statistics). Kenya population and housing census, Volume I: population by county and sub-county. Real Towers, Upper Hill, Nairobi; 2019.


Talaro KP. Foundations in microbiology. macgraw Hill, New York, 681; 2009.

Sani FM, Nasir IA, Torhile G. Mycological evaluation of smoke-dried fish sold at Maiduguri Metropolis, Nigeria: Preliminary findings and potential health implications. Europian Journal of Health sciences. 2016;2:5-10.

Akande GR, Tobor JG. Improved utilization and increased availability of fishing products as an effective control of aggravated animal protein deficiency induced malnutrition in Nigeria. Proceedings of the 10th annual conference of the fisheries society of Nigeria. 2012;18-31.

Jonathan SG, Esho EO. Fungi and Aflatoxin detection in two oyster mushrooms Pleurotus ostreatus and Pleurotus pulmonarius from Nigeria. Electronic Journal of Environmental, Agricultural and Food Chemistry. 2010;9:1722–1730.

Leggieri MC, Decontardi S, Bertuzzi T, Pietri A, Battilani P. Modeling growth and toxin production of toxigenic fungi signalled in cheese under different temperature and water activity regimes. Toxins (Basel). 2017;9(1):4.

NFSMI (National Food Service Management Institute). Safe Food Storage. The University of Mississippi; 2005.

Mutegi CK, Wagacha JM, Kimani J, Otieno G, Wanyama R, Hell K et al. Incidence of aflatoxin in peanuts (Arachis hypogaea Linnaeus) from markets in Western, Nyanza and Nairobi Provinces of Kenya and related market traits. Journal of Stored Products Research. 2013;52:118-127.

Udoh JM, Cardwel KF, Ikotun T. Storage structures and aflatoxin content of maize in five agro-ecological zones of Nigeria. Journal of Stored Products Research. 2000;36:187–201.

Rahmianna AA, Taufiq A, Yusnawan E. Effect of harvest timing and postharvest storage conditions on aflatoxin contamination in groundnuts harvested from the Wonogiri regency in Indonesia. SAT ejournal. 2007;5:1-3.

Sanders TH, Shubert AM, Pattee HE. Maturity methodology and postharvest physiology. In: Pattee, H.E., Young, C.T. (eds) Peanut science and technology. American peanut research and education society inc, Yoakum, 1982;625-627.

Muga FC, Marenya M, Workneh TS. Effect of temperature, relative humidity and moisture on aflatoxin contamination of stored maize kernels. Bulgarian Journal of Agricultural Science. 2019;25(2):271-277.

Turner PC, Sylla A, Gong YY, Diallo MS, Sutcliffe AE, Hall AJ, Wild CP. Reduction in exposure to carcinogenic aflatoxins by postharvest intervention measures in West Africa: A community based-intervention study. Lancet. 2005;365:1950-1956.

Hell K, Mutegi C. Aflatoxin control and prevention strategies in key crops of Sub-Saharan Africa. African Journal of Microbiology Research. 2011;5:459-466.

Bankole SA, Adebanjo A. Mycotoxins in food in West Africa: current situation and possibilities of controlling it. African Journal of Biotechnology. 2003;2(9):254-263.

Wu F, Khlangwiset P. Health economic impacts and cost-effectiveness of aflatoxin reduction strategies in Africa: Case studies in biocontrol and postharvest interventions. Food Additives & Contaminants. 2010; 27:496-509.

Ouedraogo AS, Oueda A, Gneme A, Savadogo GL, Barro N, Kabre GB. Handling conditions and microbial contamination of fish from Ouagadougou markets in Burkina Faso. African Journal of Microbiology Research. 2018; 12(35):866-872.

Akaki KD, Sadat AW, Coulibaly K. Bacteriological study of grilled fish consumed in yamoussoukro commune, political capital of côte d'ivoire. Current Research Journal of Biological Sciences. 2012;4(3):297-300.

Barro N, Bello A, Savadogo A, Ouattara CA, Ilboudo AJ, Traoré A. Hygienic status assessment of dish washing waters, utensils, hands and pieces of money from street food processing sites in Ouagadougou (Burkina Faso). African Journal of Biotechnology. 2006;5(11): 1107-1112.

Abolagba OJ, Uwagbai EC. A comparative analysis of the microbial load of smoke-dried fishes (Ethmalosa fimbriata and Pseudotolithus elongatus) sold in Oba and Koko markets in Edo and Delta States, Nigeria at different seasons. Australian Journal of Basic and Applied Sciences. 2011;5(5):544-550.

Adejumo, TO, Adejoro DO. Incidence of aflatoxins, fumonisins, trichothecenes and ochratoxins in Nigerian foods and possible intervention strategies. Food Science and Quality Management. 2014;31:127–146.