Optimization of Mycological Media Using Agro Waste for the Production of Antimicrobial Substance

Main Article Content

Evangeline Ogonna Okpalauwaekwe
Chinelo Ursula Umedum
Ikechukwu Harmony Iheukwumere
Leona Chisara Akakuru

Abstract

Aim: This present study was conducted to optimize mycological media using agro waste for the production of antimicrobial substance.

Place and Duration of Study: Agro waste (sugarcane and sweet potato, sugarcane and jack fruit) collected within Anambra state between February- August 2019.

Methodology: Sugarcane and sweet potato (AMSSP), sugarcane and jack fruit (AMSJ) were peeled and the peels were air-dried and then ground into powdered form. 10 g each of the agro waste samples was weighed into 400 ml of distilled water in 1000 ml Erlenmeyer flask and allowed for 7 days, after which the mixture was filtered.

Then 200 ml of the filtrate was used.

The experimental conditions were optimized by using agro wastes (20/80 and 50/50 concentrations) as a culture medium, altering the temperature (30ºC and 37ºC), pH (5, 6, 7, 8, and 9), as well as the carbon and nitrogen source (glucose and NaNO3). The fungi used were Aspergillus niger, Aspergillus fischeri, Aspergillus aculeatus and Aspergillus fumigatus.

Results:  Various agro wastes medium AMSSP and AMSJ were formulated as mycological media and the growth and nutritional conditions were optimized to ascertain antimicrobial substance production using some fungal isolates. Based on different concentrations Aspergillus fumigatus showed a promising zone of inhibition on AMSSP at a concentration of 20/80 while in AMSJ the concentration the 50/50 showed a maximum zone of inhibition on Aspergillus fumigatus ascertaining the presence of antimicrobial substance. AMSSP was able to produce maximum antimicrobial substance when supplemented with 1.0% glucose, 1.0% NaNO3 at pH 7 and at temperature of 30 ± 2ºC.

Conclusion: Agro wastes from AMSSP as well as from AMSJ contain nutrients that may support fungal growth. Maximum antimicrobial substance production is enhanced when supplemented with 1.0% of the carbon and nitrogen source at a pH of 7 and at a temperature of 30 ± 2ºC.

Keywords:
Agro waste, antimicrobial agent, optimization, zones of inhibition.

Article Details

How to Cite
Okpalauwaekwe, E. O., Umedum, C. U., Iheukwumere, I. H., & Akakuru, L. C. (2020). Optimization of Mycological Media Using Agro Waste for the Production of Antimicrobial Substance. Microbiology Research Journal International, 30(8), 67-73. https://doi.org/10.9734/mrji/2020/v30i830252
Section
Original Research Article

References

Akharaiyi FC, Abiola MA. Isolation and cultivation of fungi with agro waste formulated media. Derpharma Chemical. 2016;8(9):56–62.

Keller NP. Fungal secondary metabolism: Regulation fungi and drug discovery. Nature Reviews Microbiology. 2018;17:167-180.

Fabrizio A, Foster DG, Bailey MA. Natural products from filamentous fungi and production of heterologous expression. Applied Microbiology and Biotechnology. 2017;101:493–500.

Saravanan P, Muthuvelayudham R, Viruthagiri T. Enhanced production of cellulase from pineapple waste by response surface methodology. Journal of Engineering. 2012;2013:8.

Papaioannou EH, Liakopoulou-Kyriakides M. Agro-food wastes utilization by Blakeslea trispora for carotenoids production. Acta Biochimica Polonica. 2012;59(1):151–153.

Kakde PR, Aithal SC. Production of cellulases through solid state fermentation (SSF) using agricultural waste biomass as solid substrates by Aspergillus niger. International Journal of Scientific Research in Biological Sciences. 2019;5(4):8- 11.

Naqvi SHA, Dahot MU, Kham MY, Xu JH, Rafiq M. Usage of sugarcane bagasse as an energy source for the production of lipase by Aspergillus fumigates. Pakistan Journal of Botany. 2013;45(1): 279–284.

Arushdeep S, Umar F. Sugarcane bagasses: A potential medium for fungal cultures. Chinese Journal of Biology. 2014;4:1–5.

Hooi LH, Lee YL. Bio processing of agricultural wastes as optimized carbon source and optimization of growth conditions for xylanase production by Aspergillus brasiliensis in agitated solid state fermentation. Journal of Biodiversity, Bio Prospecting and Development. 2014;1(3):1–12.

Suganthi R, Benazir JF, Santhi R, Ramesh KV, Anjana H, Nitya M, Nidhiya KA, Kavitha G, Lakshmi R. Amylase produced by Aspergillus niger under solid state fermentation using agro-industrial wastes. International Journal of Engineering Science and Technology. 2014;3(2):1756–1763.

Durowade KA, Kolawole OM, Uddin II RO, Enonbun KI. Isolation of ascomycetous fungi from a Tertiary Institution campus soil. Journal of Applied Sciences and Environmental Management. 2010;12(4): 57–61.

Watanabe T. Pictorial atlas of soil and seed fungi. Morphologies of cultured fungi and key to species. Lewis Publishers, London, Washington D.C. 2010;410– 421.

Mulamattathil SG, Bezuidenhout C, Mbewe M, Ateba CN. Isolation of environmental bacteria from surface and drinking water in Mafikeng, South Africa and characterization using their antibiotic resistance profile. Journal of Pathogens; 2014.

Adeel R, Shabir AK, Ali A, Mohammed S, Imran A, Fazal U, Rozina R, Gulmakeri S, Mohammed A. Isolation and identification of antibiotics producing microorganisms from soil. International Journal of Pharmaceutical Sciences and Research. 2017;9(3):1002–1011.

Iheukwumere IH, Umedum CU. Effect of Gossypium hirsutum leaf extracts on gram negative bacteria isolated from cervix of females with unexplained infertility. African Journal of Science. 2013;14:3261–3270.

Smith HA. Production of antimicrobials and antioxidants from filameatous fungi. 2014;11–13.

Umedum CU, Enejekwute NP. Exploration of fungi growth on media formulated from agro-allied wastes. Tropical Journal of Applied Natural Sciences. 2017;2(1):69–73.