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Aim: This study was carried out to determine the prevalence and antimicrobial susceptibility of bacteria and fungi isolated from wounds of in-patients with road traffic accidents at four General hospitals in Niger State.
Place and Duration of Study: Department of Microbiology, Federal University of Technology, Minna, Nigeria, between October 2017 and May 2018.
Methodology: Wound swabs were taken twice per patient, first swab at contact and second swab taken seven days post wound dressing. The outcome of 1000 wound swabs taken from 409 (267 male and 142 female) in-patients with road traffic accident wound treated in the General hospitals Bida, Minna, Kontagora and Suleja areas of Niger State, Nigeria.
Results: The results showed that 405 (40.5%) samples were positive for bacteria and fungi pathogens while 595 (59.5%) were negative. 262 (80.6%) swabs yielded single isolates while 46 (14.2%) yielded double organisms and 17 (5.2%) yielded three or more organisms. Of the 405 positive cultures, 347 (85.7%) were bacterial isolates while 58 (14.3%) were fungal isolates. Staphylococcus aureus predominates (37.7%), followed by Pseudomonas aeruginosa (15.6%), E. coli (11.4%), Streptococcus pyogenes (9.4%), Bacillus subtillis (7.2%), Klebsiella pneumonia (4.4%), Aspergillus niger (4.2%), Candida albicans (3.7%), Aspergillus flavus (2.7%), Candida pseudotropicallis (2.2%) and Mucor pusillus (1.5%).
Conclusion: Most bacterial isolates were sensitive to Levofloxacin, Ciprofloxacin, Streptomycin and Gentamycin, while others showed some degree of resistance to the remaining antibiotics. Klebsiella pneumoniae showed the highest resistance to all the antibiotics used. All the fungi isolated were highly sensitive to the antifungal drugs used except Griseofulvin.
Odero W, Khayes M, Heda PM. Road traffic injuries in Kenya: Magnitude, causes and status of intervention. Inju Contr Saf Prom. 2003;10:53-61.
Porter SJ, Greavis IK. Major trauma. UK: Oxford University Press; 2010.
Palmer C. Major trauma and the injury severity score—where should we set the bar? Annual Proceedings of the Association for the Advancement of Automotive Medicine. 2007;51:13–29.
[PMC 3217501, PMID 18184482]
Bowler PG, Duerden BI, Amstrong DC. Wound microbiology and associated approaches to wound management. Clin Microb Rev. 2001;14:244–269.
Dionigi R, Rovera F, Dionigi G, Imperator A, Ferrari A, Dionigi R. Risk factors in surgery. J Chemoth. 2001;13:6-11.
Iroha IR, Amadi ES, Orji JO, Esimone CO. In vitro evaluation of the activity of colloidal silver concentrate against Pseudomonas aeruginosa isolated from postoperative wound infection. Sci Res Essay. 2008;3(5): 209-211
Akinjogunla OJ, Adegoke AA, Mboto CI, Chukwudebelu IC, Udokang IP. Bacterio-logy of automobile accident wounds infection. Int J Med Med Sci. 2009;1(2):23-27.
Federal Road Safety Commission: Nigerian traffic accidents (January to October, 2009). FRSC report 2009. Abuja: Federal Road Safety Commission; 2009.
Shittu AO, Kolawole DO, Oyedepo ER. A study of wound infections in two health institutions in Ile-Ife, Nigeria. Afr J Bio Res. 2002;5:97-110.
Buchanan KE, Gibons NE. Bergey’s manual of determinative bacteriology. (8th ed.) Williams and Williams Company, Baltimoe; 1974.
Cheesbrough M. Microscopical techniques used in microbiology. In district laboratory practice in tropical countries (Part 2). Third edition United Kingdom: Cambridge University Press; 2010.
Barrow GI and Feltham RKA. Bacterial characters and characterization. In: Cowan and Steel's manual for identification of medical bacteria (3rd ed.) Cambridge University Press. The Edinburgh Building, Cambridge CB2 2RU, UK; 2003.
Clinical and laboratory standards institute CLSI). Performance standards for antimicrobial susceptibility testing; Twenty-third informational supplement. CLSI document M100-S23. CLSI, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA; 2013.
Reller LB, Weinstein M, Jorgensen JH, Ferraro MJ. Antimicrobial susceptibility testing: A review of general principles and contemporary practices. Clin. Inf. Dis. 2009;49(1):1749–1755.
Landis SJ. Chronic wound infection and antimicrobial use. Advanced Skin Wound Care. 2003;21:531-540.
Stotts NA. Wound infection: Diagnosis and management. In: Bryant R, Nix D (Eds). Acute and chronic wounds: Current management concepts. 4th ed. St. Louis, MO: Elsevier Mosby; 2012.
Chang D. Comparison of crash fatalities by sex and age group. Traffic safety facts, National center for statistics and analysis, 1200 New Jersey Avenue SE., Washington, DC. 2008;1-6.
Anbumani N, Kalyan J and Mallike M. Epidemiology and microbiology of wound infections. Ind J Prac Doc. 2006;3(5):55–73.
Isibor JO, Oseni A, Eyaufe A, Osagie R, Turay A. Incidence of aerobic bacteria and Candida albicans in post-operative wound infections. Afr J Micr Res. 2008;2:288-291.
Tong SY, Davis JS, Eichenberger E, Holland TL, Fowler VG. Staphylococcus aureus infections: Epidemiology, patho-physiology, clinical manifestations, and management. Clin. Microbiol. Rev. 2015; 28(3):603-61.
Rasigade JP, Vandenesch F. Staphylococcus aureus: A pathogen with still unresolved issues. Infect. Genet. Evol. 2014;21:510-514.
Edokpayi J, Oruko R, Odiyo J. The role of leather microbes in human health [Online first], Intech Open; 2019.
Allison DL, Willems HME, Jayatilake JAMS, Bruno VM, Peters BM, Shirtliff ME. Candida-bacteria interactions: Their impact on human disease. Microbiol Spectr. 2016; 4:103–136.
Schlecht LM, Peters BM, Krom BP, et al. Systemic Staphylococcus aureus infection mediated by Candida albicans hyphal invasion of mucosal tissue. Microbiology (Reading, Engl). 2015;161(1):168–181.
Malani PN, McNeil SA, Bradley SF, Kauffman CA. Candida albicans sternal wound infections: A chronic and recurrent complication of median sternotomy. Clin Infect Dis. 2002;35(11):1316-20.
Fair RJ, Tor Y. Antibiotics and bacterial resistance in the 21st century. Perspect Medicin Chem. 2014;6:25–64.
Podschun R, Ullmann U. Klebsiella spp. as Nosocomial pathogens: Epidemiology, taxonomy, typing methods, and patho-genicity factors. Clin Microbiol Rev. 1998; 11(4):589–603.
Martinez-Martinez L, Pascual A, Jacoby GA. Quinolone resistance from a trans-ferable plasmid. Lancet. 1998;351(9105): 797–9.
Sobue S, Sekiguchi K, Nabeshima T. Intracutaneous distributions of fluconazole, Itraconazole, and griseofulvin in guinea pigs and binding to human stratum corneum. Amer Soci Micro. 2004;48(1): 216-223.