Antioxidant and Antibacterial Activity of Aqueous and Alcoholic Stem Bark Extracts of Kaya Senegalensis (Desv) A. Juss., in Human Urinary Infections
Kamagate Tidiane *
Laboratory of Biotechnology and Valorisation of Agro-Resources and Natural Substances, UFR of Biological Sciences, University Peleforo Gon Coulibaly, P.O. Box 1328, Korhogo, Côte d’Ivoire and Laboratory of Biochimical Pharmacodynamy, UFR of Biosciences, Félix Houphouët Boigny University, 22 P.O. Box 582, Abidjan 22, Côte d’Ivoire.
Gboko Abiba Ouattara
Laboratory of Biotechnology and Valorisation of Agro-Resources and Natural Substances, UFR of Biological Sciences, University Peleforo Gon Coulibaly, P.O. Box 1328, Korhogo, Côte d’Ivoire and Laboratory of Biochimical Pharmacodynamy, UFR of Biosciences, Félix Houphouët Boigny University, 22 P.O. Box 582, Abidjan 22, Côte d’Ivoire.
Kassim Dosso
Laboratory of Physiology and Pharmacology, Faculty of Environment, University of Jean Lorougnon Guede of Daloa, 02 P.O. Box 150, Daloa 02, Côte d’Ivoire.
Toure Abdoulaye
Laboratory of Biotechnology and Valorisation of Agro-Resources and Natural Substances, UFR of Biological Sciences, University Peleforo Gon Coulibaly, P.O. Box 1328, Korhogo, Côte d’Ivoire and Laboratory of Biochimical Pharmacodynamy, UFR of Biosciences, Félix Houphouët Boigny University, 22 P.O. Box 582, Abidjan 22, Côte d’Ivoire.
Boni Ahoussi Pascal
Laboratory of Biotechnology and Valorisation of Agro-Resources and Natural Substances, UFR of Biological Sciences, University Peleforo Gon Coulibaly, P.O. Box 1328, Korhogo, Côte d’Ivoire.
Kablan Ahmont Landry Claude
Laboratory of Constitution and Reaction of Matter, UFR Structural Sciences of Matter and Technology, Félix Houphouët-Boigny University, 22 P.O. Box 582, Abidjan 22, Côte d’Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Urinary infections constitute a public health problem today and the responsible germs increasingly express their strong resistance to common antimicrobials. The use of plants or plant products in fight against these pathologies are considered to be a goood alternative. The objective of this study is to determine in vitro antioxidant power and antibacterial activity of aqueous and hydro-ethanolic extracts 70% of stem bark of Kaya senegalensis (Meliaceae) on clinical strains of E.coli and Staphylococcus spp. urinary infections and on two reference strains. The antioxidant activity was demonstrated by the ABTS radical cation trapping method using trolox as a reference. Sensitivity of strains to the two extracts and antibacterial parameters which are minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined respectively by punch well method in Mueller Hinton agar and liquid dilution method. Both extracts expressed a strong antioxidant power around 50 μmol TE/L of extract. The MICs varied from 6.25 mg/mL to 12.5 mg/mL for aqueous extract and from 6.25 mg/mL to 25 mg/mL for hydro-ethanolic extract. For all the strains MBC was identical to the MIC indicating that the two extracts have bactericidal power on the strains excluding the reference strain of S.aureus. These results suggest that K. senegalensis could be an alternative in the fight against urinary infections.
Keywords: K.senegalensis, antioxidant, antibacterial parameters, urinary infections