Diversity and Phylogeny of Symbiotic Bacteria Nodulating Common Bean (Phaseolus vulgaris L.) in Côte d’Ivoire

Marie-Paule Aboueuh Atse *

Agrovalorization Laboratory, Department of Biochemistry-Microbiology, UFR Agroforestry, Jean Lorougnon Guédé University (UJLoG), BP 150 Daloa, Ivory Coast and National Agronomic Research Center (CNRA), Food Crops Research Station, Vegetable and Protein Crops Program, 01 BP 633 Bouaké 01, Ivory Coast.

Mako François De Paul N’gbesso

National Agronomic Research Center (CNRA), Food Crops Research Station, Vegetable and Protein Crops Program, 01 BP 633 Bouaké 01, Ivory Coast.

Allepo Innocent Abe

Agrovalorization Laboratory, Department of Biochemistry-Microbiology, UFR Agroforestry, Jean Lorougnon Guédé University (UJLoG), BP 150 Daloa, Ivory Coast.

Noupé Diakaria Coulibaly

National Agronomic Research Center (CNRA), Food Crops Research Station, Vegetable and Protein Crops Program, 01 BP 633 Bouaké 01, Ivory Coast.

Katiénapariga Tayourou Yeo

National Agronomic Research Center (CNRA), Food Crops Research Station, Vegetable and Protein Crops Program, 01 BP 633 Bouaké 01, Ivory Coast.

Louis Butare

Alliance of Biodiversity International and CIAT. C/O CSIR - Crop Research Institute (CRI), P.O. Box 3785, Fumesua - Kumasi, Ghana.

Ibrahim Konate

Agrovalorization Laboratory, Department of Biochemistry-Microbiology, UFR Agroforestry, Jean Lorougnon Guédé University (UJLoG), BP 150 Daloa, Ivory Coast.

*Author to whom correspondence should be addressed.


Abstract

Phaseolus vulgaris L. (common bean) is an agriculturally important legume that benefits from a symbiosis with bacteria belonging to the genus Rhizobium. Growing interest in the use of rhizobia as biofertilizers has led to the identification of a large number of rhizobia strains and studies of their diversity. Although much research has been carried out on rhizobia, there is little data on the diversity of rhizobia associated with common bean in Côte d'Ivoire. This study assessed the species diversity of common bean nodulating bacteria in ivorian soils. This diversity was assessed based on 16S rRNA gene sequencing. Ten high-performance bacterial isolates extracted from common bean nodules were used for genetic analysis. The 16S rRNA gene sequences of the native isolates were closely affiliated with members of the genera Rhizobium, Bradyrhizobium, Allorhizobium and Sinorhizobium demonstrating the presence of a diversity of native bean nodule bacteria. This study also reports for the first time the presence of Allorhizobium taibaishanense in common bean nodules. These results constitute an important step in the development of an effective microbial inoculum and sustainable food production.

Keywords: Diversity, rhizobia, common bean, sequencing, 16S rRNA, phylogeny


How to Cite

Atse, Marie-Paule Aboueuh, Mako François De Paul N’gbesso, Allepo Innocent Abe, Noupé Diakaria Coulibaly, Katiénapariga Tayourou Yeo, Louis Butare, and Ibrahim Konate. 2024. “Diversity and Phylogeny of Symbiotic Bacteria Nodulating Common Bean (Phaseolus Vulgaris L.) in Côte d’Ivoire”. Microbiology Research Journal International 34 (7):45-53. https://doi.org/10.9734/mrji/2024/v34i71457.