In vitro Antifungal Efficacy of Silver Nanoparticles against Fusarium oxysporum f. sp. Lycopersici in Tomato

Shendge V. S.

Department of Plant Pathology, College of Agriculture, Latur, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani - 431 402 (MS), India.

Sunita J. Magar *

Department of Plant Pathology, College of Agriculture, Latur, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani - 431 402 (MS), India.

S.D. Somwanshi

Department of Plant Pathology, College of Agriculture, Latur, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani - 431 402 (MS), India.

*Author to whom correspondence should be addressed.


Abstract

Present investigation on Mycosynthesis of silver nanoparticles and antifungal activity of silver nanoparticles against Fusarium oxysporum f. sp. lycopersici in tomato was carried out at department of Plant Pathology, College of Agriculture, Latur to find out antifungal activity of silver nanoparticles against Fusarium oxysporum f. sp. lycopersici in tomato.

Mycosynthesis of silver nanoparticles produced from the fungus Trichoderma harzianum. Characterization of silver nanoparticles were carried out by UV-Vis spectroscopy and Transmission Electron Microscopy (TEM) which revealed that synthesized nanoparticles were having the UV absorption peak at 420 nm and nanoprticle size was 50 nm. Silver nanoparticles demonstrated significant antifungal activity against Fusarium oxysporum f. sp. lycopersici in tomato by using Agar well diffusion method and Poisoned food technique.

In Agar well diffusion method, the highest zone of inhibition 18.66 mm was recorded at 100 ppm concentration than other treatments. In poisoned food technique, the suspension of silver nanoparticles at 100 ppm concentration recorded highest (75.19%) inhibition. This was followed by 50 ppm, 30 ppm, 10 ppm conc. and Trichoderma culture filtrate which recorded 66.67%, 58.89%, 54.45% and 51.45% inhibition, respectively.

Growth of the Fusarium oxysporum f. sp. lycopersici in tomato decreased drastically with increase in the concentration of the silver nanoparticles.

Keywords: Antifungal activity, silver nanoparticles [AgNPs], Trichoderma harzianum, Fusarium oxysporum f. sp. Lycopersici, UV-Vis spectroscopy, Transmission Electron Microscopy [TEM]


How to Cite

Shendge V. S., Magar , S. J., & Somwanshi, S. (2024). In vitro Antifungal Efficacy of Silver Nanoparticles against Fusarium oxysporum f. sp. Lycopersici in Tomato. Microbiology Research Journal International, 34(3), 1–9. https://doi.org/10.9734/mrji/2024/v34i31432

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References

Khabat V, Mansoori GA, Sedighe Karimi. Biosynthesis of silver nanoparticles by fungus Trichoderma reesei. Int. sci. J. 2011;1(1):65-19.

Kaur P, Thakur R, Choudhary A. An in vitro study of the antifungal activity of silver/chitosan nanoformulation against important seedborne pathogens. Inter. J. of Sci. and Tech. Res. 2012;1:6.

Nene YL, Thapliyal PN. Evaluations of fungicides in fungicides for plant disease control (3rd ed.) Oxford, IBH Publishing Co. New Delhi. 1993;531-532.

Vincent JM. Distortion of fungal hypha in the presence of certain inhibitors. Nature. 1947;159:850.

Bissett J. A revision of the genus Trichoderma. II. Infrageneric classification. Can. J. Bot. 1991;69:2357-2372.

Tripathi RM, Gupta RK, Shrivastav A, Singh MP, Shrivastav BR, Singh Priti. Trichoderma koningii assisted biogenic synthesis of silver nanoparticles and evaluation of their antibacterial activity. Adv. Nat. Sci.: Nanosci. Nanotechnol. 2013;1-5.

Shelar GB, Chavan AM. Myco-synthesis of silver nanoparticles from Trichoderma harzianum and its impact on germination status of oil seed. Biolife. 2015;3(1):109-113.

Lamsal K, Sang Woo Kim, Jin Hee Jung, Yun Seok Kim, Kyoung Su Kim, Youn Su Lee. Application of silver nanoparticles for the control of Colletotrichum species in vitro and pepper anthracnose disease in field. Mycobiol. 2011;39(3):194-199.

Kaman PK, Dutta P. Synthesis, characterization and antifungal activity of biosynthesized silver nanoparticle. Indian Phytopathology. 2018;72(1):79-88.

Hassan SA, Hanif E, Khan UH, Tanoli A. Antifungal activity of silver nanoparticles from Aspergillus niger. Pakistan Journal of Pharmaceutical Sciences. 2019;32(3): 1163-1166.

Tomah AA, Almer IS, Li B, Zhang J. Mycosynthesis of silver nanoparticles using screened Trichoderma isolates and their antifungal activity against Sclerotinia sclerotiorum. Nanomaterials. 2020; 10: 1955.

Noshad A, Iqbal M, Folkers L, Hetherington C, Khan A, Numan M, Ullah S. (). Antibacterial effect of silver nanoparticles (AgNPs) synthesized from Trichoderma harzianum against Clavibacter michiganensis. Journal of Nano Research. 2019;58:10-19.