Microbial Analysis and Antibiogram of Bacteria and Fungi Isolated from Meat Sellers Tables in Dutsin-Ma Metropolis, Katsina State

Wata I. *

Medical Unit, Department of Microbiology, Federal University Dutsin-Ma, Katsina State, Nigeria.

Abdullahi K.

Environmental Unit, Department of Microbiology, Federal University Dutsin-Ma, Katsina State, Nigeria.

Umaru Abdulmalik

Medical Unit, Department of Microbiology, Federal University Dutsin-Ma, Katsina State, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

The goal of the research project was to carry out microbiological analysis and antibiogram of  bacteria and fungi associated with meat vendor tables in the city of Dutsin-Ma. This study made use of twenty samples in total. Five distinct locations at Wednesday Market and Darawa were chosen, and two samples from each table were taken using swab sticks. For analysis, the samples were then brought to the Federal University of Dutsin-ma microbiology laboratory department. The agars that were initially employed to isolate the bacteria and fungus were MacConkey, nutrient, mannitol salt, bile esculin, salmonella-shigella, eosin methylene blue, and potato dextrose agars. Bacterial species were identified using gram staining and biochemical tests while lactophenol cotton blue staining was used for the identification of fungal species. Bacterial isolates identified include Escherichia coli, Salmonella species, Klebsiella pneumonia, Bacillus subtilis, Streptococcus species and Staphylococcus aureus while the fungal isolates are Aspergillus flavus, A. niger, A. fumigatus, and Oidiodendron griseum. The antimicrobial susceptibility test findings showed that while some of the bacterial isolates were responsive to certain drugs, others were resistant. The results of this study's investigations therefore demonstrated how heavily polluted meat vendors' tables are with a wide variety of bacteria. This can be explained by the unsanitary procedures used in the meat sale industry as well as other environmental issues.  Therefore, it is imperative that the government authorities tasked with these duties make sure that the vendors of these meat products adhere to standard and hygienic procedures.  Raising public knowledge of the risks of foodborne illnesses associated with eating raw meats is another important goal of public health awareness campaigns.

Keywords: Antibiogram, bacterial and fungal isolates, hygiene


How to Cite

Wata I., Abdullahi K., & Abdulmalik , U. (2023). Microbial Analysis and Antibiogram of Bacteria and Fungi Isolated from Meat Sellers Tables in Dutsin-Ma Metropolis, Katsina State. Microbiology Research Journal International, 33(11), 22–31. https://doi.org/10.9734/mrji/2023/v33i11-121414

Downloads

Download data is not yet available.

References

Ramaswamy V, Cresence VM, Rejitha JS, Lekshmi MU, Dharsana KS, Prasad SP, Vijila HM. Listeria-review of epidemiology and pathogenesis. Journal of Microbiology Immunology and Infection. 2007;40(1):4.

Bacchil VN. Enterotoxigenicity, phage typing and prevalence of Staphylococcus aureus in buffalo meats.Public health implications.Indian Journal of Comparative Microbiology Immunology and Diseases. 1998;19:23-7.

Bacha K, Mehari T, Ashenafi M. Microbiological study of wakalim, a traditional Ethiopian fermented sausage. Ethiopian Journal of Biological Sciences. 2007;6(2).

Cheesbrough M. District laboratory practice in tropical countries.IJMS. 2018; 1(1):65-8.

Divya KS, Murthy SM, Jogaiah S. Ecological studies of fungal biodiversity in freshwater and their broad-spectrum applications. In Biocontrol Agents and Secondary Metabolites. Woodhead Publishing. 2021;631-648.

Mojisola O, Osho AT, Diala CI. Evaluation of microbial hazards associated with the processing of Suya (a grilled meat product) Edema. Scientific Research and Essay. 2008;3(12):621-626.

Van der Vorst JG, Da Silva CA, Trienekens JH. Agro-industrial supply chain management: Concepts and applications. FAO Agricultural management, Marketing and Finance; 2007.

Corry JE, Curtis GD, Baird RM, editors. Handbook of culture media for food microbiology. Elsevier; 2003.

Vanoverberghe K, Vanden Abeele F, Mariot P, Lepage G, Roudbaraki M, Bonnal JL, Mauroy B, Shuba Y, Skryma R, Prevarskaya N. Ca2+ homeostasis and apoptotic resistance of neuroendocrine-differentiated prostate cancer cells. Cell Death & Differentiation. 2004;11(3):321-30.

Mahajan M. Conventional methods for direct examination of fungi. Systemic fungal infections: Principles, Pathogenesis & Practice. 2019;19.

Abiola C, Oyetayo VO. Research Article isolation and biochemical characterization of microorganisms associated with the fermentation of Kersting's groundnut (Macrotyloma geocarpum). Journal of Microbiology. 2016;11:47-55.

Bayot ML, Bragg BN. Antimicrobial susceptibility testing; 2019.

Bukar A, Yusha’u M, Adikwu EM. Incidence and identification of potential pathogens on hands of personnel in some small scale food industries in Kano Metropolis. Biol. Environ. Sci. J. Trop. 2009;6(4):4–6.

Edema MO, Sanni LO, Sanni AI. Evaluation of maize-soybean flour blends for sour maize bread production in Nigeria. African Journal of Biotechnology. 2005;4 (9).

Kalalou I, Faid M, Ahami AT. Extending the shelf life of fresh minced camel meat at ambient temperature by Lactobacillus delbreuckii subsp. Delbreuckii.Journal of food production.2004;37:11-16.

Okonkwo IO, Ogumusi TA, Ogubjobi AA, et al. Microbial studies on frozen during processed in Ibadan and Lagos, Nigeria. Scientific research and essay. 2008;3(11): 537-546

Iroha IR, Ugbo I, lang DC. Bacteria contamination of raw meat sold in Abakaliki, Ebonyi state Nigeria. Journal of Public Health and Epidemiology. 2011;3(2): 49-53.

Ercolini D, Federica Torrieri, E. et al. Changes in spoilage-related microbiota of beef during refrigerated storage under different packaging conditions. Appl. Environ. Microbiol. 2009;72:4663-4671.

Akano O, Moro DD, Deji-Agboola AM, et al. Public health implication of listeria species and other bacteria isolates of abattoir effluent in Lagos Nigeria, international research journal of microbiology. 2013;4(7): 162-167.

Fasanmi GO, Olukole SG, Kehinde OO. Microbial studies of table scrapings from meat stalls in Ibadan Metropolis, Nigeria: Implications on meat hygiene. African Journal of Biotechnology. 2010;9(21): 3158-62.

Keeffe EB, Dieterich DT, Steve-huy BH, Jacobson IM, Martin P, Schiff ER, Tobias H, Wright TL. A treatment algorithm for the management of chronic hepatitis B virus infection in the United States. Clinical Gastroenterology and Hepatology. 2004; 2(2):87-106.

Sofos JN, Kochevar SL, Bellinger GR, Buege DR, Hancock DD, Ingham SC, Morgan JB, Reagan JO, Smith AC. Sources and extent of microbiological contamination of beef carcasses in seven United States slaughtering plants. Journal of food protection. 1999;62(2): 140-5.