Bacteriological Evaluation of Indoor Air Quality in Some Selected Units at the University of Cape Coast Hospital in Ghana

Geoffrey Saah Botchway *

Department of Laboratory Technology, School of Physical Sciences, University of Cape Coast, Ghana.

Hubert Danquah Nyarko

Department of Laboratory Technology, School of Physical Sciences, University of Cape Coast, Ghana.

Juliana Amoah

Department of Laboratory Technology, School of Physical Sciences, University of Cape Coast, Ghana.

Emmanuel Birikorang

Department of Laboratory Technology, School of Physical Sciences, University of Cape Coast, Ghana.

*Author to whom correspondence should be addressed.


Abstract

A hospital is an environment solely for diagnosing and treating patients. Contemporary research, however, reveals the possibility of users contracting diseases due to many factors, such as poor air quality. This research, therefore, delves into the critical indoor air quality assessment domain, focusing on selected units within the University of Cape Coast Hospital, Ghana. The study’s primary objective was to conduct a comprehensive microbial assessment of indoor air quality in eight different units (emergency room, operating theater, out-patient department, consulting rooms, laboratory, male ward, female ward, and ear, nose, and throat unit) of the hospital, shedding light on potential airborne bacteria present. Indoor and outdoor air were sampled using Koch’s sedimentation method. Colony forming units per cubic meter of air (cfu/m3) were determined with the Omeliansky formula. The bacteriological load within the units revealed that the out-patient department had the highest bacterial concentration (139.2±60.32×102 cfu/m3), immediately followed by Outdoor (135.1±43.63×102 cfu/m3), whereas ear, nose, and throat unit recorded the least concentration (0.4±0.57×102 cfu/m3). The remaining units range between 135.1±43.63×102 cfu/m3 and 0.4±0.57×102 cfu/m3. The morphological characteristics of the seven observed bacterial isolates (GSB 1-7) showed the presence of two cocci and five rods. Isolates 1 and 4 had a rhizoid form, isolates 2,3, and 5 had a circular form, while isolates 6 and 7 had filamentous forms. All isolates showed positive gram tests, and endospores were detected in isolates 1, 4, and 6. Bacterial isolates were identified as Bacillus mycoides, Micrococcus luteus, Staphylococcus epidermidis, Bacillus circulans, Staphylococcus saprophyticus, Bacillus subtilis, and Micrococcus sp. These outcomes indicate bacterial contaminations in the indoor environment, likely to pose a significant risk to patients, workers, and visitor’s safety. Therefore, rigorous monitoring and mitigation strategies are essential to ensure a safer environment in healthcare settings.

Keywords: Bacteria, microorganisms, contaminants, culture, indoor air quality


How to Cite

Botchway , G. S., Nyarko , H. D., Amoah, J., & Birikorang , E. (2023). Bacteriological Evaluation of Indoor Air Quality in Some Selected Units at the University of Cape Coast Hospital in Ghana. Microbiology Research Journal International, 33(11), 1–8. https://doi.org/10.9734/mrji/2023/v33i11-121411

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References

United States Environmental Protection Agency. Introduction to Indoor Air Quality. Accessed 14 September 2023.Available at: https://www.epa.gov/indoor-air-quality-iaq/introduction-indoor-air-quality.

Sikora A, Zahra F. Nosocomial infections. StatPearls. StatPearls Publishing. 2022;23:16.

Kalwasinska A, Burkowska A, Wilk I. Microbial air contamination in indoor environment of a university library. Annals of Agricultural and Environmental Medicine. 2012;19(1):25-29.

Mandal J, Brandl H. Bioaerosols in indoor environment-a review with special reference to residential and occupational locations. The Open Environmental & Biological Monitoring Journal. 2011 Sep 28;4(1):83-96.

Poza et al. Exploring bacterial diversity in hospital environments by GS-FLX Titanium pyrosequencing. PloS one. 2012; 7(8):e44105.

Sarıca S, Asan A, Otkun MT, Ture M. Monitoring indoor airborne fungi and bacteria in the different areas of Trakya university hospital, Edirne, Turkey. Indoor and Built Environment. 2002 Dec 11;11(5):285-292.

van Doremalen et al. Aerosol and surface stability of HCoV-19 (SARS-CoV-2) compared to SARS-CoV-1. medRxiv: the preprint server for health sciences. 2020 Mar;09:20033217.

Ibrahim F, Samsudin EZ, Ishak AR, Sathasivam J. Hospital indoor air quality and its relationships with building design, building operation, and occupant-related factors: A mini-review. Frontiers in public health. 2022 Nov 8;10:1067764.

Chadeganipour M, Shadzi S, Nilipour S, Ahmadi G. Airborne fungi in Isfahan and evaluation of allergenic responses of their extracts in animal model. Jundishapur Journal of Microbiology, University of Medical Sciences, Isafan. 2010;3(4):155-60.

Krikor Jilenkerian BE, Nisafi A, Kara Ali AH, Aleissa B. FIRST study of the impact of the Syrian natural Zeolite on air biological contamination concentrations in Broiler farms during spring and Autumn. Asian Journal of Advances in Research. 2022 Sep 27;5(1):1107-15

Asem E, Sabuli N, Nyarko H. Assessment of fungal propagules in some selected banking halls of the university of Cape Coast community, Ghana. British Microbiology Research Journal. 2016;11(3):1-8.

Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST, editors. Bergey’s manual of determinative bacteriology. 9th ed. Baltimore: Williams and Wilkins. 1994;559.

CEC. Biological particles in indoor environments. Report No. 12. Luxembourg: Commission of the European Communities; 1994.

World Health Organization. Indoor air quality: biological contaminants: report on a WHO meeting, Rautavaara, 29 August–2 September 1988. World Health Organization. Regional Office for Europe; 1990.

Jensen PA, Schafer MP. Sampling and characterization of bioaerosols. NIOSH manual of analytical methods. 1998 Dec 12;1(15):82-112.

Gizaw Z, Gebrehiwot M, Yenew C. High bacterial load of indoor air in hospital wards: the case of university of Gondar teaching hospital, Northwest, Ethiopia. Multidisciplinary Respiratory Medicine. 2016 Dec;11(24):4-6.

Enoch KL, Jacob NA, Stephen WK, Courage KS. Microbial load of indoor airborne bacteria and fungi in a teaching hospital in Ghana. African Journal of Microbiology Research. 2020 Mar 31;14(3):100-5.

Choo-Smith et al. Investigating microbial (micro) colony heterogeneity by vibrational spectroscopy. Applied and environmental microbiology. 2001 Apr 1;67(4):1461-9.

Dalton KR, Rock C, Carroll KC, Davis MF. One Health in Hospitals: how understanding the dynamics of people, animals, and the hospital built-environment can be used to better inform interventions for antimicrobial-resistant gram-positive infections. Antimicrobial Resistance & Infection Control. 2020 Dec;9:1-7.

Setlow P. Germination of spores of bacillus species: what we know and do not know. Journal of bacteriology. 2014 Apr 1;196(7):1297-305.

Nicholson WL, Munakata N, Horneck G, Melosh HJ, Setlow P. Resistance of bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiology and Molecular Biology Reviews. 2000 Sep 1;64(3):548-72.

Yafetto L, Adator EH. Fungal contaminations of indoor and outdoor air of buildings of the university of Cape Coast, Ghana. Studies in Fungi. 2018;3(1):333-42.

Jawetz E, Brooks GF, Carroll KC, Butel JS, Morse SA, Mietzner TA. Jawetz, Melnick, & Adelberg’s medical microbiology. 28th ed. McGraw-Hill Education; 2019.