Circulation of Crimean-Congo Haemorrhagic Fever Virus in Ticks in Upper Guinea-Republic of Guinea
Mamadou Gando Diallo *
Département de Biologie, Faculté des Sciences, Université de Kindia, BP: 212, Republique de Guinée.
Aissatou Boiro
Institut de Recherche en Biologie Appliquée Guinée-Kindia, BP: 146, République de Guinée.
Bonaventure Kolie
Département de Biologie, Faculté des Sciences, Université de Kindia, BP: 212, Republique de Guinée.
Ekaterina Naydenova
Institut de Recherche en Biologie Appliquée Guinée-Kindia, BP: 146, République de Guinée.
Abdoulaye Djibril Diallo
Département de Biologie, Faculté des Sciences, Université de Kindia, BP: 212, Republique de Guinée.
Souleymane Diallo
Département de Biologie, Faculté des Sciences, Université de Kindia, BP: 212, Republique de Guinée.
Thierno Amadou Labé Balde
Institut de Recherche en Biologie Appliquée Guinée-Kindia, BP: 146, République de Guinée.
*Author to whom correspondence should be addressed.
Abstract
The aim of this study was to map the distribution of agents carrying the Crimean-Congo haemorrhagic fever virus (arbovirus-tica) in the natural region of Upper Guinea. The prefectures of Kankan, Dabola and Faranah were used as collection areas. Random sampling of different types of animals was used to collect the biomaterial. Two types of analysis methods (RT-PCR and ELISA) were used. Out of a total of 578 ticks collected and divided into 254 pools, the genus Amblyomma was the most frequently encountered with 83 tick pools. Molecular analysis (RT-PCR) for the detection of virus RNA revealed 2 positive cases (0.8%). Direct enzyme-linked immunosorbent assays (ELISA) for the detection of Ag yielded only one positive case (0.4%). We found that the species Rhipicephalus geigyi was the main vector and reservoir of the pathogen in Upper Guinea.
Keywords: CCHF, ticks, RT-PCR, ELISA, Upper Guinea
How to Cite
Downloads
References
Bente DA, Forrester NL, Watts DM, McAuley AJ, Whitehouse CA, Bray M. Crimean-Congo hemorrhagic fever: History, epidemiology, pathogenesis, clinical syndrome and genetic diversity. Antiviral Research. 2013;100:159–189. Available:https://doi.org/10.1016/j.antiviral.2013.07.006
Gétaz L, Loutan L, Mezger N. Diseases transmitted by ticks locally and abroad. Rev Med Suisse. 2012;8(340):974-6.
K. Senior Vector-borne diseases threaten Europe. Lancet Infect Dis. 2008;(8) [Medline].
Africa Office of the World Health Organization (WHO); National Health Services of Scotland, Fitfortravel; 2019.
Naidenova EV, Zakharov Kartachov KS, Agafonov DA, Senichkina AM, N'Faly Magassouba, Nourdine I, Nassour AA, Bah MB, Kourouma A, Boumbali S, Boiro MON, Scherbakova SA, Kutyrev VV, Dedkov VG. Prevalence of Crimean-Congo hemorrhagic fever virus in rural areas of Guinea; 2020. Available:https://doi.org/10.1016/j.ttbdis.2020.101475 N. 20-64-46014
Farhadpour F, Telmadarray Z, Chinikar S, Akbarzadeh K, Moemenbellah-Fard MD, Faghihi F, Fakoorziba M, Jalali T, Mostafavi E, Shahhosseini N, Mohammadien M. Online publication February 11, 2016. Molecular detection of Crimean-Congo hemorrhagic fever virus in ticks collected from infested livestock populations in a new endemic area, southern Iran Affiliations expand. 2016;21(3):340-7. PMID: 26758985. DOI: 10.1111/tmi.12667
Aillen E O’Heam, Matthew A Voorhees, David P Fetterer, Nadia Wauquier, Moinya R Coomber, Jams Bangura, Joseph N Fair, Jean Paul Gonzalez, Randal J. Schoepp. Serosurveillance of viral pathogens circulating in West Africa in O’Heam et al. Virology Journal. 2016; 13:163.
Sanidad M, Sociales e Igualdad S. Status report and assessment of the risk of transmission of Crimean-Congo hemorrhagic fever (CCHF) in Spain; 2017. [in Spanish] [cited 2017 May 1] Available:http://www.msssi.gob.es/profesionales/saludPublica/enfermedadesEmergentes/Crimea_Congo/docs/ACTUALIZACION_ER_FHCC_20.04.2017.pdfExternal Link
Baumann D, Pusterla N, Péter O, et al. Fever after a tick bite: Clinical manifesta tions and diagnosis of acute tick bite-associated infections in Northeas tern Switzerland. Dtsch Med Wochenschr. 2003;128:1042 7.
Jacques Morvan, Published on February 8 2020 at 9:43 a.m. Outbreak of Crimean-Congo hemorrhagic fever in Mali Travel medicine (promedmail).