Comparative Roles of HBB5 Biosurfactant and Poultry Wastes in Polyaromatic Hydrocarbon Biodegradiation of Crude Oil-contaminated Sediment
Microbiology Research Journal International,
The comparative study of poultry wastes- and HBB5 biosurfactant-mediated polyaromatic hydrocarbon biodegradation in sediment polluted with crude oil were investigated. The experiments were carried out for a period of 28 days by monitoring pH, nitrate, phosphate, polycyclic aromatic hydrocarbon and microbiological parameters using standard procedures. The pH values obtained ranged between 6.21 and 6.93 in days 1 and 28 for the most effective treatment recipes. Generally, there was depletion in the concentrations of nitrate and phosphate for all set ups, but the most effective recipe witnessed highest reduction. For the polycyclic aromatic hydrocarbons, the recipe with highest limiting nutrients depletion also recorded the most hydrocarbon loss, and yet highest increase in density of hydrocarbonoclastic bacteria and fungi. The sample containing polluted sediment + poultry wastes + HBB5 biosurfactant recorded PAH values of 1932.6472ppm on day 1 and 481.2272ppm on day 28. Total hydrocarbon-utilizing bacterial counts ranged from 1.48×104 cfu/g to 9.70×106 cfu/g, while hydrocarbon-utilizing fungal counts ranged between 2.30×103 cfu/g and 3.90×105 cfu/g. From the results obtained, poultry wastes combined with HBB5 biosurfactant recorded the highest efficiency in the biodegradation of polycyclic aromatic hydrocarbons in sediments, and HBB5 biosurfactant in isolation recorded higher degradation efficiency for polyaromatic hydrocarbons than the degradation effect mediated by poultry wastes alone. It is therefore recommended that a combination of surface-active agent, nutrient amendment source and viable microbial biomass be adopted and employed as potent recipe for the degradation of polyaromatic hydrocarbons in crude oil-contaminated sediments.
- poultry wastes
- polyaromatic hydrocarbon
- crude oil-contaminated sediment
How to Cite
Cappello S, Caruso G, Zampino D, Monticelli LS, Maimone G, Denaro R, Tripodo B, Troussellier M. Microbial community dynamics during assays of harbour oil spill bioremediation: A microscale simulation study. Journal of Applied Microbiology. 2007a;102:184–194.
Catania V, Santisi S, Signa G, Vizzini S, Mazzola A, Cappello S, Yakimov MM, Quatrini P. Intrinsic bioremediation potential of a chronically polluted marine coastal area. Marine Pollution Bulletin. 2015;99:138–149.
Maisano M, Cappello T, Natalotto A, Vitale V, Parrino V, Giannetto A, Oliva S, Mancini G. Effects of petrochemical contamination on caged marine mussels using a multi-biomarker approach: Histological changes, neurotoxicity and hypoxic stress. Marine Environmental Research. 2017;128:114–123.
Cerneglia CE. Biodegradation of organic contaminants in sediments: Over-view and examples with polycyclic aromatic hydro-carbons. Chapter 16, this volume; 1992.
Gao YZ, Zhu LZ. Plant uptake, accumulation and translocation of phenanthrene and pyrene in soils. Chemosphere. 2004;55:1169–1178.
Haeseler F, Blanchet D, Druelle V, Vandecasteele JP. Analytical characterization of contaminated soils from former manufactured gas plants. Environmental Science and Technology. 1999;33(6):825–830.
Meador JP, Stein JE, Reichert WL, Varanasi U. Bioaccumulation of polycyclic aromatic hydrocarbons by marine organisms. Review in Environmental Contamamination and Toxicology. 1995; 143:79–165.
Juhasz AL, Naidu R. Bioremediation of high molecular weight polycyclic aromatic hydrocarbons: A review of the microbial degradation of benzo[a]pyrene. Int. Biodeterioration Biodegradation. 2000;45: 57–88.
Bargiela R, Mapelli F, Rojo D, Chouaia B, Tornes J, Borin S. Bacterial population and biodegradation potential in chronically crude oil contaminated marine sediments are strongly linked to temperature. Sci. Rep. 2015;5:11651.
Bauer JE, Capone DG. Effects of co-occurring aromatic hydrocar- bons on the degradation of individual polycyclic aromatic hydrocarbons in marine sediment slurries. Applied Environmental Microbiology. 1988;54:1649–1655.
Heitkamp MA, Cerniglia CE. Effects of chemical structure and exposure on the microbial degradation of polycyclic aromatic hydrocarbons in freshwater and estuarine ecosystems. Environmental Toxicology and Chemistry. 1987;6535–546.
Anora NK. Bioremediation: A green approach for restoration of polluted ecosystems. Environ. Sustain. 2018;1: 305–307.
Pawar RM. The effect of soil pH on bioremediation of polycyclic aromatic hydrocarbon (PAHS). Bioremediation and Biodegradation. 2015;6(3):1–14.
Conde Molina D, Liporace F, Quevedo C. Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil. Biotech. 2022;3(12):118.
Bragg JR, Prince RC, Harver EJ, Atlas RM. Effectiveness of bioremediation for Exxon Valdez oil spill. Nature. 1994;413–418.
Mata-Sandoval J, Karns J, Torrent J. Influence of rhamnolipids and Triton X-100 on the desorption of pesticides from soils. Environmental Science and Technology. 2002;36:4669–4675.
Bordas F, Lafrance P, Villemur R. Conditions for effective removal of pyrene from an artificially contaminated soil using Pseudomonas aeruginosa 57SJ rhamnolipids. Environmental Pollution. 2005;138:69–76.
Ite AE, Ibok UJ, Ite MU, Petters SW. Petroleum exploration and production: Past and present environmental issues in the Nigeria’s Niger Delta. American Journal of Environmental Protection. 2013; 1:78–90.
American Public Health Association (APHA). Standard Methods for examination of water and waste water. American Public Health Association, 20th Edition. 1998;113.
Prescott LM, Harley JP, Klien DA. Microbiology, 6th ed. McGraw Hill London. 2005;135–140.
Odokuma LO, Dickson AA. Bioremediation of a crude oilpolluted tropical rain forest soil. Global Journal of Environmental Science. 2003;2:29–40.
Nkwocha IL, Odokuma LO. Monoaromatic Hydrocarbon bioremediation of hydrocarbon-contaminated soil using HBB5 Biosurfactant produced by Pseudomonas xiamenensis isolated from the brackish water of AmadiAma creek in Port Harcourt Rivers State. Journal of Advances in Microbiology. 2021;12(7):7–18.
Obire O, Wemedo SA. The effect of oil field wastewater on the microbial population of soil in Nigeria. Niger Delta Biologia. 1996;1:77–85.
Singh BK, Walker A, Morgan JAW, Wright DJ. Role of soil pH in the development of enhanced biodegradation of fenamiphos. Applied and Environmental Microbiology. 2006;69(12):7035– 7043.
Leahy JG, Colwell RR. Microbial degradation of hydrocarbons in the environment. Microbiology Reviews. 1990; 54:305–315.
Varjani SJ, Upasani VN. Biodegradation of petroleum hydrocarbons by oleophilic strain of Pseudomonas aeruginosa NCIM 5514. Bioresources Technology. 2016;222: 195–201.
Boopathy R. Factors limiting bioremediation technologies. Bioresource Technology. 2000;74:63–67.
Orecchio S, Polizzotto G. Fractionation of mercury in sediments during draining of Augusta (Italy) coastal area by modified Tessier method. Microchem J. 2013;110: 452-457.
Romano E, Bergamin L, Ausili A, Celia Magno M, Gabellini M. Evolution of the anthropogenic impact in the Augusta Harbor (Eastern Sicily, Italy) in the last decades: benthic foraminifera as indicators of environmental status. Environmental Science Pollution Research International. 2016;23:10514–10528.
Signa G, Mazzola A, Di Leonardo R, Vizzini S. Element specific behavior and sediment properties modulate transfer and bioaccumulation of trace elements in a highly contaminated area (Augusta Bay, Central Mediterranean Sea). Chemosphere. 2017;187:230–239.
Rulkens WH, Bruning H. Cleanup technologies for dredged fine sediments: Review and future challenges. Finding achievable risk reduction solutions remediation of contaminated sediments, C6-01; 2005.
Genovese M, Crisafi F, Denaro R, Cappello S, Russo D, Calogero R, Santisi S, Catalfamo M. Effective bioremediation strategy for rapid in situ cleanup of anoxic marine sediments in mesocosm oil spill simulation. Front Microbiology. 2014;5:162.
Foght JM, Fedoral PM, Westlake DWS. Mineralization of 14C-phenanthrene in crude oil: Specificity among bacterial isolates. Canadian Journal of Microbiology. 1990;36(3):169–175.
Roubal G, Atlas RM. Distribution of hydrocarbon utilizing microorganisms and hydrocarbon biodegradation potentials in Alaskan continental shelf area. Applied and Environmental Microbiology. 1978;35: 897–905.
Sikkema J, de Bont JAM, Poolman B. Mechanisms of membrane toxicity of hydrocarbons. Applied and Environmental Microbiology. 1995;59:201–222.
Tuveson RW, Kagan J, Shaw MA, Moresco GM, Behne EMV, Pu H, Basin M, Santus R. Phototoxic effects of fluoranthene, a polycyclic aromatic hydrocarbon, on bacterial species. Environmental and Molecular Mutagenesis. 1987;10:245–261.
Abstract View: 44 times
PDF Download: 10 times