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Nitrogen-Fixing and Phosphate Solubilizing Potentials of Rhizobacteria from the Rhizosphere of Two Cassava Varieties in Iyamho Community

  •   Ehis-Eriakha Chioma Bertha
  •   Akemu Stephen Eromosele
  •   Akharaiyi Fred Coolborn

Microbiology Research Journal International, Volume 32, Issue 7, Page 51-60
DOI: 10.9734/mrji/2022/v32i71334
Published: 20 October 2022

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Abstract


Microorganisms in close association with the roots of plants can enhance plant growth, through nitrogen fixation (NF) and phosphorus solubilization (PS). Although the type of microbes in close association with different plants varies, their population and genetic capabilities is affected by several factors. Therefore, in this study, the plant growth promoting properties of rhizobacteria present in the rhizosphere of two cassava varieties (Sweet cassava US, bitter cassava ST) indigenous to Iyamho community was explored.   The samples were analyzed for total culturable heterotrophic bacteria community and the obtained isolates were screened for NF and PS abilities using a semi-solid N-free medium and Pikovaskya agar respectively. The bacterial population in both agar medium varied, however, the bacterial counts on Luria Bertani (3.67 x 105, 3.35 x 106) was higher than  Nutrient agar (2.73 x 105, 2.68 x 105) after incubation for 24 hours at 37oC  for sweet and bitter cassava rhizosp here respectively. Also, isolates from sweet cassava had the highest bacteria count in both Nutrient agar and Luria Bertani agar. A total of sixteen isolates were obtained, six phosphate solubilizers, five nitrogen fixers, and five without traits for either NF or PS. The Gram-negative bacterial group was more dominant across all isolates while the dominant genus was Bacillus. This study indicates that the nitrogen fixers and phosphate solubilizers are major constituents of the rhizomicrobe of cassava plants although the distribution varies across cassava varieties. However, sweet cassava rhizosphere harbored more nitrogen-fixing bacteria while both varieties had the same amount of phosphate solubilizing rhizobacteria.

Keywords:
  • PGPR
  • rhizobacteria
  • nitrogen-fixation
  • phosphate solubilization
  • cassava
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How to Cite

Bertha, E.-E. C., Eromosele, A. S., & Coolborn, A. F. (2022). Nitrogen-Fixing and Phosphate Solubilizing Potentials of Rhizobacteria from the Rhizosphere of Two Cassava Varieties in Iyamho Community. Microbiology Research Journal International, 32(7), 51–60. https://doi.org/10.9734/mrji/2022/v32i71334
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References

Food and Agriculture Organisation of the United Nations (FAO). Save and grow: Cassava, a guide to sustainable production and intensification. Rome; 2013.

McKey D, Cavagnaro TR, Cliff J, Gleadow R. Chemical ecology in coupled human and natural systems: People, manioc, multitrophic interactions and global change. Chemoecology. 2010;20:109-133.

Otekunrin OA, Sawicka B. Cassava, a 21st century staple crop: How can Nigeria harness its enormous trade potentials?. Acta Scientific Agriculture. 2019;3(8): 194-202.

De Almeida LG, Candian J, Cardoso AII, Grassi Filho H. Nitrogen, phosphorus and potassium content of six biofertilizers used for fertigation in organic production system. Comunicata Scientiae. 2021; 12e3275-e3275.

Li YB, Liu XM, Hao TY, Chen SF. Colonization and maize growth promotion induced by phosphate solubilizing bacterial isolates. International Journal of Molecular Sciences. 2017;18(7):1253.

Sharma SB, Sayyed RZ, Trivedi MH, Gobi TA. Phosphate solubilizing microbes: Sustainable approach for managing phosphorus deficiency in agricultural soils. Springer Plus. 2013;2(1):587.

Geiger F, Bengtsson J, Berendse F, Weisser WW, Emmerson M, Morales MB, Inchausti P. Persistent negative effects of pesticides on biodiversity and biological control potential on european farmland. Basic and Applied Ecology. 2010;11(2): 97-105.

Schindler DW, Hecky RE, Findlay DL, Stainton MP, Parker BR, Paterson MJ, Beaty KG, Lyng M, Kasian SE. Eutrophication of lakes cannot be controlled by reducing nitrogen input: Results of a 37-year whole-ecosystem experiment. Proceedings of the National Academy of Sciences of the United States of America. 2008;105(32):11254-11258.

Belay E, Teshome B. Diversity of rhizobacteria associated with sorghum wild relatives in assosa zone of Ethiopia and their ability to solubilize phosphate. Frontiers in Environmental Microbiology. 2021;7(2):63.

Khan N, Bano A, Rahman MA, Rathinasabapathi B, Babar MA. UPLC HRMS based untargeted metabolic profiling reveals changes in chickpea (Cicer arietinum) metabolome following long term drought stress. Plant Cell Environ; 2018.

DOI:10.1111/pce.13195

Frediansyah A. The microbiome of cassava (Manihot esculanta). Cassava: Biology, Production and Use. 2021;33.

Melo FMP, Fiore MF, Moraes LABD, Silva-Stenico ME, Scramin S, Teixeira MDA. Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4A. 2009;66(5):583-92.

Leite M, Pereira A, Souza A, Andreote F, Freire F, Sobral J. Bioprospection and genetic diversity of endophytic bacteria associated with cassava plant. Revista Caatinga. 2018;31(2):315-325.

Xie J, Shi H, Du Z, Wang T, Liu X, Chen S. Comparative genomic and functional analysis reveal conservation of plant growth promoting traits in Paenibacillus polymyxa and its closely related species. Scientific Reports. 2016;6(1):21329.

Pueschel D, Janouskova M, Voriskova A, Gryndlerova H, Vosatka M, Jansa J. Arbuscular mycorrhiza stimulates biological nitrogen fixation in two medicago spp. through improved phosphorus acquisition. Frontiers in Plant Science. 2017;8(e0154116):390.

Kumar A, Maurya BR, Raghuwanshi R, Meena VS, Tofazzal Islam M. Co-inoculation with enterobacter and rhizobacteria on yield and nutrient uptake by wheat (Triticum aestivum L.) in the alluvial soil under indo-gangetic plain of India. Journal of Plant Growth Regulation 2017;36(3):608-617.

Pikovskaya RI. Mobilization of phosphorous in soil in the connection with vital activity of some microbial species. Mikorobiologiya. 1948;17:362–370.

Ehis-Eriakha CB, Ezeanya-Bakpa CC, Akemu SE, Yahaya SO. Assessment of Indigenous rhizospheric soil microbes from zea mays and manihot esculenta for plant growth promoting (PGP) traits. Archive of Science & Technology. 2020;1:88–97.

Ghevariya KK, Desai PB. Rhizobacteria of sugarcane: In vitro screening for their plant growth promoting potentials. Research Journal of recent science. 2014;3:53-58.

Ju W, Jin X, Liu Shen L. Rhizobacteria inoculation benefits nutrient availability for phytostabilization in copper contaminated soil: Drivers from bacterial community structures in rhizosphere. Applied Soil Ecology. 2020;150.

Available:10.1016/j.apsoil.2019.103450

Goswami D, Thakker J, Dhandhukia P. Portraying mechanics of plant growth promoting rhizobacteria (PGPR): A review. Cogent Food & Agriculture. 2016;2: 1127500.

Gouda S, Kerry R, Samal D, Mahapatra G Das, Patra JK. (). Application of plant growth promoting rhizobacteria in agriculture. In: Pradeep K, Jayanta KP, Pranjal C. (Eds). Advances in Microbial Biotechnology Current Trends and Future Prospects. Apple Academic Perss. 2018; 73-83.

Available:10.1201/9781351248914-3

Figueiredo MDVB, Bonifacio A, Rodrigues AC, Araujo FFD. Plant growth-promoting rhizobacteria: Key mechanisms of action. In Microbial-mediated induced systemic resistance in plants Springer, Singapore. 2016;23-37.

Gupta G, Parihar SS, Kumar AN, Kumar SS, Singh V.Plant growth promoting rhizobacteria (PGPR): Current and future prospects for development of sustainable agriculture. Journal of Microbiology and Biochemistry Technology. 2015;7:2.

Chinakwe E, Mberede C, Ngumah C. Plant growth promoting bacteria from local and hybrid maize (Zea mays) varieties. Annals food science and technology. 2019;20(1): 387-392.

Sibponkrung S, Kondo T, Tanaka K, Tittabutr P, Boonkerd N, Yoshida KI, Teaumroong N. Co-inoculation of Bacillus velezensis strain S141 and bradyrhizobium strains promotes nodule growth and nitrogen fixation. Microorganisms. 2020;8 (5):678.

Safriani SR, Fitri L, Ismail YS. Isolation of potential plant growth promoting rhizobacteria (PGPR) from cassava (Manihot esculenta) rhizosphere soil. Biosaintifika: Journal of Biology & Biology Education. 2020;12(3):459- 468.

Miljaković D, Marinković J, Balešević-Tubić S. The significance of Bacillus spp. in disease suppression and growth promotion of field and vegetable crops. Microorganisms. 2020;8(7): 1037.

Moustaine M, Elkahkahi R, Benbouazza A, Benkirane R, Achbani EH. Effect of plant growth promoting rhizobacterial (PGPR) inoculation on growth in tomato (Solanum lycopersicum L.) and characterization for direct PGP abilities in Morocco. International Journal of Environment, Agriculture and Biotechnology. 2017;2(2): 238708.

Ke X, Feng S, Wang J, Lu W, Zhang W, Chen M, Lin M. Effect of inoculation with nitrogen-fixing bacterium Pseudomonas stutzeri A1501 on maize plant growth and the microbiome indigenous to the rhizosphere. Systematic and Applied Microbiology. 2019;42(2):248-260.

Sezonov G, Joseleau-Petit D, D'Ari R. Escherichia coli physiology in luria-bertani broth. Journal of bacteriology. 2007; 189 (23):8746–8749.

Available:https://doi.org/10.1128/JB.01368-07

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