Microbiology Research Journal International 2019-11-16T13:09:48+00:00 Microbiology Research Journal International Open Journal Systems <p style="text-align: justify;"><strong>Microbiology Research Journal International (ISSN:&nbsp;2456-7043)</strong> is dedicated to publish research papers, reviews, and short communications in all areas of Microbiology such as virology, mycology, parasitology, bacteriology, clinical microbiology, phycology, parasitology, protozoology, microbial physiology, immunology, microbial genetics, medical microbiology, microbial pathogenesis and epidemiology disease pathology and immunology, probiotics and prebiotics</p> Degradation Kinetics of Microbial Consortium Isolated from Diesel Oil Impacted Soil in Delta Park, University of Port Harcourt, Nigeria 2019-11-16T06:39:06+00:00 Ogochukwu Ann Udume Enobong Effiong Gideon Orkwagh Abu <p>Heavy-duty generators constitute a form of hydrocarbon pollution but enrich microorganisms to having degradative ability of hydrocarbons hence can be used for remediation. In this study a laboratory treatability (biodegradation screening) study was employed to investigate the hydrocarbon degradation competence of bacteria and fungi. Culture-dependent microbiological and physicochemical analyses was conducted on the soil samples obtained from the polluted site. The total aerobic heterotrophic bacterial and fungi counts increased from 5.0-7.5 (Log<sub>10 </sub>cfu/mL) between day 0-14 then reduced to 6.5 Log<sub>10 </sub>cfu/mL. The hydrocarbon-utilizing bacteria increased from 4.5-5.2 (Log<sub>10 </sub>cfu/mL). Percentage degradation hydrocarbons, attributed to fungi bacteria and bacteria/fungi consortia, were 42.3%, 54% and 70% respectively while the control had 6.0%. The total petroleum hydrocarbon (TPH) removal rate (K) was modelled using the first order kinetics:&nbsp; y=-0.0398x+9.79; K=0.0398d<sup>-1</sup>. These results, correspond to a degradation efficiency of 70% and t<sub>1/2 </sub>of 17 days for the bacterial/fungal consortium. The K values for the other setups were 0.019 d<sup>-1</sup>, 0.0261 d<sup>-1</sup> and 0.0022 d<sup>-1</sup> with the corresponding degradation efficiencies of 42%, 54% and 6.01% and half-life of 37 days, 27 days and 315 days for fungi, bacteria and control respectively. This result indicates that the use of microbial consortia has high potentials in remediation of hydrocarbons and other pollutants of concern.</p> 2019-11-09T00:00:00+00:00 ##submission.copyrightStatement## Isolation, Screening and Characterization of Exopolysaccharide Producing Bacteria 2019-11-16T13:09:48+00:00 I. G. Nwosu G. O. Abu K. O. Agwa <p>Exopolysaccharides(EPSs) are essential metabolites synthesized and excreted by certain microorganisms in response to extreme condition of pH, temperature, salinity, osmotic stress and other contaminants for survival in such adverse environment. The present study focuses on isolation of exopolysaccharide producing bacteria from extreme environment of oil polluted soil of Ogoni land and marine water of Bonny Island. Screening of EPS producing abilities of the selected isolates were estimated using two approaches <em>viz </em>gravimetric analysis of EPS dry weight and quantification assay for total carbohydrate content by phenol sulphuric acid method. Result revealed that a total of forty (40) different colonies were suspected to produce exopolysaccharide after preliminary screening by selecting thick ropy-like colony formers on agar medium. Secondary screening indicated that twelve (12) isolates produced precipitates above 1000mg/l of total dry weight and eight (8) isolates produced highest EPS yield above 1000mg/l of culture media with carbohydrate content determination. Five isolates with the code WAS1, WAS11, SC6, SOS7 and SOS10 produced significantly higher EPS compared to other bacterial colonies isolated and were termed as most potent EPS producers. These isolates were identified based on 16S rDNA sequence as<em> Providencia stuarti, Escherichia coli, Shewanella chilikensis </em>and<em> Bacillus nealsonii</em>. The result of the present study indicated that these strains have the potentials of producing high exopolysaccharides and can be explored in biotechnological industries.</p> 2019-11-16T00:00:00+00:00 ##submission.copyrightStatement##