Nasal Carriage of Methicillin-Resistant Staphylococcus aureus in Intensive Care Units of Two University Hospitals in Cameroon

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Hortense Gonsu Kamga
Yves Le Grand Napa Tchuedji
Emilia Lyonga Mbamyah
Jérémie Djiraibe
Anicette Chafa Betbeui
Michel Noubom
Bonaventure Jemea
Paul Owono Etoundi
Arthur Essomba


Aim: The aim of this work was to carry out a screen for methicillin-resistant Staphylococcus aureus (S. aureus) in nasal cavity of patients in the intensive care units of the University Hospital Center (UHC) and the Central Hospital of Yaounde (CHY) in Cameroon.

Study Design: A cross-sectional descriptive study was carried out.

Pace and Duration of the Study: Collection of nasal swab was done in Intensive Care Unit of University Teaching Hospital of Yaounde, and Intensive Care Unit of Central Hospital of Yaounde. Identification and susceptibility test were done in bacteriology laboratory of University Hospital Center, Yaounde between August 2018 and March 2019.

Methods: Nasal swabs were collected from patients by performing rotation in each nose. The identification of bacteria was carried out by observation of mannitol fermentation on Chapman agar, catalase, coagulase and DNAse tests. The susceptibility test was carried out by the method of diffusion of the discs in Mueller-Hinton agar.

Results: A total of 29 S. aureus were identified from 127 patients of which 44.10% were women  and 55.90% were men. The antibiotic resistance profile showed cross-resistance of S. aureus between cefoxitin and others antibiotics with high resistance of amoxicillin, Amoxi / Clavulanic, fusidic acid, gentamycin and tetracycline with rates ranging from 62% to 82%. We detected 58.62% resistant species to cefoxitin and 51.72% were resistant to vancomycin. Statistical analysis found that there was not association between age groups, gender with nasal carriage of S. aureus. However there was an association (P=0,0060) between the hospital attended and the portage of S. aureus.

Conclusion: The prevalence of Methicillin-resistant S. aureus (MRSA) is quite high in intensive care patients. Staphylococcus aureus isolated from carriers also shows resistance to others antibiotics. This can increase the incidence of nosocomial infections. There is a need to implement effective control strategies to prevent infection cross transmission in intensive care Units.

Antibiotic resistance, MRSA, Staphylococcus aureus, nosocomial infections.

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How to Cite
Kamga, H. G., Tchuedji, Y. L. G. N., Mbamyah, E. L., Djiraibe, J., Betbeui, A. C., Noubom, M., Jemea, B., Etoundi, P. O., & Essomba, A. (2020). Nasal Carriage of Methicillin-Resistant Staphylococcus aureus in Intensive Care Units of Two University Hospitals in Cameroon. Microbiology Research Journal International, 30(4), 26-33.
Original Research Article


OMS. Premier rapport de l’OMS sur la résistance aux antibiotiques: une menace grave d’ampleur mondiale; 2014.

Kollef MH. Gram-negative bacterial resistance: evolving patterns and treatment Lina; 2005.

Boyce JM. Strategies for controlling methicillin-resistant Staphylococcus aureus in hospitals. J Chemother. 1995;7(Suppl 3):81–5.

Marie-Claude M. Infection nosocomiale à Staphylococcus aureusrésistant à la méthicilline chez un patient adulte hospitalisé: quell antibiotique choisir? 2012;33.

Combes A, Luyt CE, Fagon JY. Trial Group. Impact of methicillin-resistance on outcome of Staphylococcus aureus ventilator-associated pneumonia. Am J RespirCrit Care Med. 2004;170:786–792.

Clotilde Njall, Dieudonné Adiogo, André Bita, Noel Ateba, GéraldSume, BasileKollo, Fidèle Binam, Romain Tchoua. Écologie bactérienne de l’infection nosocomiale au service de réanimation de l’hôpital Laquintinie de Douala, Cameroun. Pan African Medical Journal. 2013;14:140.

Kateete DP, Kimani CN, Katabazi FA, Okeng A, Okee MS, Nanteza A, Joloba ML, Najjuka FC. Identification of Staphylococcus aureus: DNase and Mannitol salt agar improve the efficiency of the tube coagulase test. Annals of Clinical Microbiology and Antimicrobials. 2010;9: 23.

Adel K, Talbi M. Etude de la résistance du Staphylococcus aureusrésistant à la méthicilline aux autres antibiotiques. 2017; 1-12.

CA-SFM. Recommandations. 2019;1.0. 114.

Oguzkaya-Artan M, Baykan Z, Artan C, Avsarogullari L. Prevalence and risk factorsfor methicillin resistant Staphylococcus aureus carriage among emergency department workers and bacterial contamination on touch surfaces in Erciyes University Hospital, Kayseri, Turkey African H1291 ealth Sciences. 2015;15.

République Algérienne Démocratique et Populaire; Ministère de la Santé de la Population et de la Réforme Hospitalière; Réseau Algérien de Surveillance de la Résistance des Bactéries aux Antibiotiques. Surveillance de la résistance des bactéries aux antibiotiques; 2015.

Wertheim HFL, Melles DC, Vos MC, van Leeuwen W, van Belkum A, Verbrugh HA, et al. The role of nasal carriage in Staphylococcus aureus infections. Lancet Infect Dis. 2005;5:751–62.

Mohamed Amine Ouidri. New Microbe and New Infect. 2018;23:52–60.

Campbell KA, Colleen Cunningham, Saqib Hasan, Lorraine Hutzler, Joseph A. Bosco III. Risk Factors for Developing Staphylococcus aureus Nasal Colonization in Spine and ArthroplastySurgery.Bulletin of the Hospital for Joint Diseases. 2015; 73(4):276-81.

Nakatomi Y, Sugiyama J. A rapid latex agglutination assay for the detection of penicillin-binding protein 2. MicrobiolIm-munol. 1998;42:739–43.

L Metwally , N Gomaa , R Hassan. detection of methicillin-resistant Staphylococcus aureus directly by loop-mediated isothermal amplification and direct cefoxitin disk diffusion tests. East Mediterr Health J. 2014;20 (4):273-9.

Dumitrescu O. et al. Beta-lactams interfering with PBP1 induce Panton-Valentine leukocidin expression by triggering sarA and rot global regulators of Staphylococcus aureus. Antimicrob. Agents Chemother. 2011;55:3261– 3271.

Hecker MT, Aron DC, Patel NP, Lehmann MK, Donskey CJ. Unnecessary use of antimicrobials in hospitalized patients. Arch Intern Med. 2003;163:972–8.

Oliveira DC, Tomasz A, de Lencastre H. Secrets of success of a human pathogen: molecular evolution of pandemic clones of methicillin resistant Staphylococcus aureus. Lancet Infect Dis. 2002;2:180–9.