Polyethylene Biodegradation Potentials of Pseudomonas aeruginosa and Micrococcus sp. Isolated from Waste Dumps and Farmlands in Nsukka, Enugu State, Nigeria

Main Article Content

Vincent Chigor
Chidiebele Nwankwo
Uchenna Ogbodo
Joseph Ugwu

Abstract

Background: Low Density Polyethylene (LDPE) are plastic materials extensively used in packaging, constituting recalcitrant environmental pollutants that defy natural degradation processes.

Aim: This study isolated bacteria from a Nigerian environment and assessed their potential for LDPE biodegradation.

Methods: Using standard procedures, Bacteria were isolated from polythene samples collected from farmlands and waste dump sites in Nsukka metropolis. Mineral salt medium (MSM) was prepared, with LPDE as sole carbon source, and used for isolation. Optical density (OD600 nm) was used to study bacterial growth on LDPE as sole carbon source as proof of biodegradation. Both organisms demonstrated steady growth on LDPE over time.

Results: Pseudomonas aeruginosa and Micrococcus sp. were identified based on morphological and biochemical characteristics. Ability to grow on LDPE as a sole carbon source was studied as evidence of polyethylene biodegradation. Organisms were inoculated into MSM and incubated at 37°C and 50°C for 15 days. Maximum growth was recorded after 15 days of incubation for both organisms. P. aeruginosa and Micrococcus sp. showed steady growth at 37°C as well as 50 ⁰C. Micrococcus sp. recorded highest growth; 0.324 nm and 0.312 nm at 37°C and 50°C respectively, after 15 days. Similarly, P. aeruginosa recorded highest growth of 0.40 nm and 0.258 nm for 37°C and 50°C respectively. LDPE degradation increased with increase in time.

Conclusion: This study demonstrates the enormous polyethylene-degrading potentials of P. aeruginosa and Micrococcus sp. isolated from Nsukka, Nigeria.

Keywords:
Low Density Polyethylene (LDPE), P. aeruginosa, Micrococcus sp., biodegradation, contaminants

Article Details

How to Cite
Chigor, V., Nwankwo, C., Ogbodo, U., & Ugwu, J. (2020). Polyethylene Biodegradation Potentials of Pseudomonas aeruginosa and Micrococcus sp. Isolated from Waste Dumps and Farmlands in Nsukka, Enugu State, Nigeria. Microbiology Research Journal International, 30(4), 10-18. https://doi.org/10.9734/mrji/2020/v30i430208
Section
Original Research Article

References

Bailey R. Nutrient cycle through the environment. Science, Thought Co; 2019.
Available:https://www.thoughtco.com/all-about-the-nutrient-cycle-373411
(Accessed July 26, 2017)

Chmielewski J, Kusztal P, Żeber-Dzikowska I. Anthropogenic impact on the environment (case study), Environmental Protection and Natural Resources; The Journal of Institute of Environmental Protection-National Research Institute. 2018;29(1):30-37.

Tiwary MR. Impact of disposed drinking water sachets in Damaturu, Yobe State, Nigeria. Delta. 2015;16393564:345-904.

Umeh PP, Friday K, Oji S. Geographical analysis of household waste generation and disposal in Taraba state, northeast Nigeria. International Journal. 2019;8(2): 58-68.

Mian MM, Zeng X, Nasry AA, Al- Hamadani SM. Municipal solid waste management in China: A comparative analysis. Journal of Material Cycles and Waste Management. 2017;19(3):1127- 35.

Kofoworola OF. Comparative assessment of the environmental implication of management options for municipal solid waste in Nigeria. Int. J. Waste Resour. 2016;7(1):1-5.

Azarbaijani R, Yeganeh LP, Blom J, Younesi H, Fazeli SA, Tabatabaei M, Salekdeh GH. Comparative genome analysis of Oceanimonas sp. GK1, a halotolerant bacterium with considerable xenobiotics degradation potentials. Annals of Microbiology. 2016;66(2):703- 16.

Grover A, Gupta A, Chandra S, Kumari A, Khurana SP. Polythene and environment. International Journal of Environmental Sciences. 2015;5(6):1091.

Muhonja CN, Makonde H, Magoma G, Imbuga M. Biodegradability of polyethylene by bacteria and fungi from Dandora dumpsite Nairobi-Kenya. PloS One. 2018; 13(7):e0198446.

Punch News. Plastic Pollution: Nigeria’s untapped ‘waste wealth’ fuels environmental disaster; 2018.

Available:https://punchng.com/plastic-pollution-nigerias-untapped-waste-wealth-fuels-environmental-disaster/
(Accessed 26-07-19)

Adekomaya O, Ojo K. Adaptation of plastic waste to energy development in Lagos: An overview assessment. Nigerian Journal of Technology. 2016;35(4):778-84.

United States Environmental Protection Agency (EPA). Reduce, Reuse and Recycle; 2019.
Available at https://www.epa.gov/recycle
(Accessed 26-07-19)

Kyaw BM, Champakalakshmi R, Sakharkar MK, Lim CS, Sakharkar KR. Biodegradation of low density polythene (LDPE) by Pseudomonas species. Indian journal of microbiology. 2012;52(3):411-9.

Aurah MC. Assessment of Extent to Which Plastic Bag Waste Management Methods Used in Nairobi City Promote Sustainability. American Journal of Environmental Protection. 2013;1:96-101.

Pattnaik S, Reddy MV. Assessment of municipal solid waste management in Puducherry (Pondicherry), India. Resources, Conservation and Recycling. 2010;54(8):512-20.

Shankar S, Singh S, Mishra A, Sharma M. Microbial degradation of polyethylene: Recent progress and challenges. In Microbial Metabolism of Xenobiotic Compounds. Springer, Singapore. 2019; 245-262.

Paniagua-Michel J, Rosales A. Marine bioremediation: A sustainable bio-technology of petroleum hydrocarbons biodegradation in coastal and marine environments. Journal of Bioremediation & Biodegredation. 2015;6(2):1.

Kritzberg ES, Duarte CM, Wassmann P. Changes in Arctic marine bacterial carbon metabolism in response to increasing temperature. Polar Biology. 2010;33(12): 1673-82.

Mahjoubi M, Jaouani A, Guesmi A, Amor SB, Jouini A, Cherif H, Najjari A, Boudabous A, Koubaa N, Cherif A. Hydrocarbonoclastic bacteria isolated from petroleum contaminated sites in Tunisia: isolation, identification and characterization of the biotechnological potential. New biotechnology. 2013;30(6):723-33.

Skariyachan S, Manjunatha V, Sultana S, Jois C, Bai V, Vasist KS. Novel bacterial consortia isolated from plastic garbage processing areas demonstrated enhanced degradation for low density polyethylene. Environmental Science and Pollution Research. 2016;23(18):18307-18319.

Kathiresan K. Polythene and plastics-degrading microbes from the mangrove soil. Revista de biologia tropical. 2003; 51(3-4):629-33.

Pethe K, Sequeira PC, Agarwalla S, Rhee K, Kuhen K, Phong WY, Patel V, Beer D, Walker JR, Duraiswamy J, Jiricek J. A chemical genetic screen in Mycobacterium tuberculosis identifies carbon-source-dependent growth inhibitors devoid of in vivo efficacy. Nature Communications. 2010;1:57.

Hall BG, Acar H, Nandipati A, Barlow M. Growth rates made easy. Molecular biology and evolution. 2013;31(1):232-8.

Gabrielson J, Hart M, Jarelöv A, Kühn I, McKenzie D, Möllby R. Evaluation of redox indicators and the use of digital scanners and spectrophotometer for quantification of microbial growth in microplates. Journal of Microbiological Methods. 2002;50(1):63-73.

Orr IG, Hadar Y, Sivan A. Colonization, biofilm formation and biodegradation of polyethylene by a strain of Rhodococcus ruber. Applied Microbiology and Biotechnology. 2004;65(1):97-104.

Nnamchi CI, Obeta JA, Ezeogu LI. Isolation and characterization of some polycyclic aromatic hydrocarbon degrading bacteria from Nsukka soils in Nigeria. International Journal of Environmental Science & Technology. 2006;3(2):181-90.

Parte A. Bergey's manual of systematic bacteriology: The actinobacteria. Springer Science & Business Media. 2012;5.

Cheesbrough M. District laboratory practice in tropical countries. Cambridge university press; 2006.

Palleroni NJ. General properties and taxonomy of the genus Pseudomonas. Genetics and biochemistry of Pseudomonas. 1975;162:1-36.

Collins CH, Lyne PM. Laboratory techniques series: Microbiological methods. Butterworth, London; 1970.

Tankeshwar A. Bacterial Growth Curve: phases and significance, Bacteriology, Microbiology for Beginners. Microbeonline; 2013.
Available:https://microbeonline.com/typical-growth-curve-of-bacterial-population-in-enclosed-vessel-batch-culture/
(Accessed 07-08-2019)

Mohan K. Microbial deterioration and degradation of polymeric materials. Journal of Biochemical Technology. 2011;2(4): 210-5.

Koutny M, Sancelme M, Dabin C, Pichon N, Delort AM, Lemaire J. Acquired biodegradability of polyethylenes containing pro-oxidant additives. Polymer Degradation and Stability. 2006;91(7): 1495-503.

Gu JD. Microbiological deterioration and degradation of synthetic polymeric materials: recent research advances. International biodeterioration & Biodegradation. 2003;52(2):69-91.

Falade AO, Eyisi OA, Mabinya LV, Nwodo UU, Okoh AI. Peroxidase production and ligninolytic potentials of fresh water bacteria Raoultella ornithinolytica and Ensifer adhaerens. Biotechnology Reports. 2017;16:12-7.

Biswas JK, Mondal M, Rinklebe J, Sarkar SK, Chaudhuri P, Rai M, Shaheen SM, Song H, Rizwan M. Multi-metal resistance and plant growth promotion potential of a wastewater bacterium Pseudomonas aeruginosa and its synergistic benefits. Environmental Geochemistry and Health. 2017;39(6):1583-93.

Jain R, Pandey A. A phenazine-1-carboxylic acid producing polyextremophilic Pseudomonas chlororaphis (MCC2693) strain, isolated from mountain ecosystem, possesses biocontrol and plant growth promotion abilities. Microbiological Research. 2016; 190:63-71.

Khabbaz F, Albertsson AC, Karlsson S. Chemical and morphological changes of environmentally degradable polyethylene films exposed to thermo-oxidation. Polymer Degradation and Stability. 1999; 63(1):127-38.

Beresford T, Williams A. The microbiology of cheese ripening. Cheese: Chemistry, Physics and Microbiology. 2004;1:287-318.