Prevalence and Antibiogram of Gram-negative Bacteria Isolated from Well Water in Ula-Ubie Community, Ahoada West, Nigeria

Main Article Content

R. R. Nrior
M. Okpokiri
N. P. Akani

Abstract

Antibiotic resistance has become a great global problem. Thus, it has emerged as a public health challenge. The antibiotic susceptibility pattern of bacteria in well water was characterized with a view of determining the level of resistance in the environment. Fifty well water samples were collected from ten different points in Ula-Ubie community, Ahoda, Rivers State for a period of five months. Standard microbiological methods were used to analyse the population and types of bacteria in the water while methods recommended by the American Public Health Association (APHA) was used to determine the physicochemical parameters of the samples. The antibiotic susceptibility profile of the bacterial isolates was carried out using the disc diffusion methods. The total heterotrophic bacteria of the water samples ranged from 0.93±0.46 to 2.02±1.06 log10 CFU/ml. The coliform counts ranged from 0.45±0.42 - 2.55±2.33 log10 CFU/ml, respectively. Despite the variations in the counts of the different bacterial population, there was no significant differences (P > 0.05) in the different well water samples. The physicochemical parameters except the pH were all within the permissible limits. Klebsiella spp, Pseudomonas spp, Serratia spp and Enterobacter spp were identified in the well water. The pH of the water stations ranged from 4.66 to 5.80. The temperature ranged from 24.0 to 24.7. The electrical conductivity, salinity, dissolved oxygen, total hardness, alkalinity, total suspended solids, biochemical oxygen demand, nitrate, chloride, calcium and magnesium ranged from 22.9 – 219, 0.03-0.13, 4.50-4.90, 5.00-22.0, ˂0.01-3.00, ˂0.01, 49.6-84.5, 1.00-17.4, 3.00-24.5, 4.25-12.9 and 0,722-1.55 respectively. The antibiotic susceptibility profile showed that all the isolates were resistant to ceftazidime and augmentin, whereas Enterobacter spp were the most resistant bacteria amongst other bacterial genera to the antibiotics. Meanwhile there is an existence of multi-drug resistance. Thus, the wells could be considered not potable due to the presence of these bacterial isolates and the level of antibiotic resistant. Proper sanitation and cleanliness of well should be encouraged.

Keywords:
Antibiogram, gram-negative bacteria, well water, Klebsiella spp, Pseudomonas spp.

Article Details

How to Cite
Nrior, R. R., Okpokiri, M., & Akani, N. P. (2020). Prevalence and Antibiogram of Gram-negative Bacteria Isolated from Well Water in Ula-Ubie Community, Ahoada West, Nigeria. Microbiology Research Journal International, 30(2), 1-10. https://doi.org/10.9734/mrji/2020/v30i230192
Section
Original Research Article

References

Adogo LY, Anyanwu NCJ, Ajiji MA, Bukola Ajide. Bacteriological and physio-chemical analysis of borehole water in Auta Balifi Community, Nigeria. British Microbiology Journal. 2016;11(4):1-7.

Rajini Kuruf, Roland Persaud, John Ceaser, Vincent Raja. Microbiological and physiochemical analysis of drinking water George Town, Guyana. Nature and Science. 2010;8(8):261-265.

Obafemi O. Olubanjo, Alade E. Adebolu, Olubanjo M. Abosede. Bacteriological. assessment of borehole and wells water in Akungba-Akoko, Nigeria. International Journal of Agriculture, Environment and Bioresearch. 2018;3(6):2456-8643.

Palamuleni L, Akoth M. Physico-chemical and microbial analysis of selected borehole water in Mahikeng, South Africa. International Journal of Environmental Research and Public Health. 2015;12: 8619-8630.

Obire O, Aguda M, Ramesh RP. Impact of human activities on drinking water quality. Journal of Basic and Applied Biology. 2008;2(3&4):52-58.

World Health Organization. Guidelines for drinking-water quality: Fourth edition incorporating the first addendum. Geneva; 2017.

Obioma A, Chikanka AT, Loveth NW. Evaluation of bacteriological quality of surface, well, borehole and river water in Khana Local Government Area of Rivers State, Niger Delta. Ann Clin Lab Res. 2017;5(3):183.

Sur D, Sarka BL, Dean J, Delta S, Niyogi SK, et al. Epidemiological, microbiological and electron microscopic study of a cholera outbreak in a Kolkata slum community. Indian J Med Res. 2006;123: 31-36.

WHO. Guidelines for drinking water quality (3rd Edn). WHO, Geneva, Switzerland; 2003.

Prescott LM, Harley J, klein DA. Microbiology 8th Ed, McGraw-Hill New York. 2011;809-811.

Wemedo SA, Robinson VK. Evaluation of indoor air for bacteria organisms and their antimicrobial susceptibility profiles in a Government Health Institution. Journal of Advances in Microbiology. 2018;11(3):1-7.

Parkyz AL. The utility of hospital antibiograms as tools for guiding empiric therapy and tracking resistance insights from the society of infectious diseases pharmacists. Pharmacotherapy. 2007; 27(9):1306-1312.

Wemedo SA, Obire O, Akani NP. Bacterial population of an oilfield wastewater in Nigeria. Asian Journal of Biological Sciences. 2012;5:46-51.

Chesbrough M. District laboratory practice in tropical countries, Part. Cambridge University Press U.K. 2005;55-80.

Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey’s manual of determinative bacteriology. Williams and Wilkins, Baltimore, Maryland, USA. 1994;151–157.

Clinical and Laboratory Standard Institutes. Performance standards for antimicrobial disk susceptibility tests. CLSI Document M100. Clinical and Laboratory Standard Institutes, 28th Edition; 2013.

EPA. US Environment Protection Agency. Safe drinking water act ammendment; 2002.

Available:http:// www. epa. gov/safe water /mcl. Html

Azuonwu O, Azuonwu TC, Nwizug WL. Evaluation of bacteriological quality of surface, well, borehole and river water in Khana Local Government Area of Rivers State, Niger Delta. Annals of Clinical and Laboratory Research. 2017;3:183.

World Health Organization (WHO). Guideline for drinking water quality 4 Edition, WHO, Switzerland. 2011;156.

Augustín L, Adriana LS, Pedro MR, María MS. Assessment of the microbiological quality of groundwater in three regions of the Valencian Community (Spain). International Journal of Environmental Research and Public Health. 2014;11: 5527-5540.

Obire O, Osigwe IS. Bacterial quality of spring water in Ihitte/Uboma LGA of Imo State, Nigeria. Current studies in comparative education. Science and Technology. 2016;3(2):149-155.

Adeleke EO, Omafuvbe BO. Antibiotic resistance of aerobic mesophilic bacteria isolated from poultry faeces. Research Journal of Microbiology. 2011;6(4):356-365.

Suely APF, Erica MDS, Patricia FS, Paola CL, Lúcia MT. Antimicrobial resistance profiles of enterococci isolated from poultry meat and pasteurized milk in Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz, Rio de Janeiro. 2007;102(7):853-859.

Ramya R, Shanthi M, Uma S, Arunagiri K. Detection of vancomycin resistance among Enterococcus faecalis and Staphylococcus aureus. Journal of Clinical and Diagnostic Research. 2016;10(2):4-6.

Tom E, Anna C, Peter L, Jonathan S. Medical microbiology and infection (5th Edn). 2011;147.

Shittu AO, Lin J. Antimicrobial susceptibility patterns and characterization of clinical isolates of Staphylococcus aureus in KwaZulu-Natal province, South Africa. BioMed Central Infectious Diseases. 2006;125–126.

Aderibigbe SA, Awoyemi AO, Osagbami GK. Availability, adequacy and quality of water supply in Ilorin Metropolis, Nigeria. European. J. Sci. Res. 2008;23(4):528-636.

Mwekaven SS, Aorkwagh MT, Gundu EG, Yange T. Physico-chemical and micro-biological analysis of well water stations In settlements around Akperan Orshi College of Agriculture, Yandev. International Journal of Science and Technology. 2017; 6:1.

Charkhabi AH, Sakizadeh M. Assessment of spatial variation of water quality; 2006.

Baird C, Cann M. Environmental chemistry (3rd Edition). W. H. Freeman, USA; 2004.

Ajit MK, Padmakar AS. Determination of physico-chemical parameters of deoli bhorus dam water. Advances in Applied Science Research. 2012;3(1):273-279.