Effects of Nitrogen and Carbon Sources on Biosurfactant Production by Hydrocarbon-utilizing Stenotrophomonas sp.

Main Article Content

Victor Ezebuiro
Ipeghan Jonathan Otaraku
Boma Oruwari
Gideon Chijioke Okpokwasili

Abstract

Aim: This study investigated effects of nitrogen and carbon sources on the production of biosurfactant by a hydrocarbon-utilizing bacterium, Stenotrophomonas sp.

Methodology: The hydrocarbon-utilizing bacterium was isolated with Bushnell Haas (BH) broth using enrichment method. Biosurfactant production was screened by evaluating the following characteristics: Emulsification index (E-24), oil spreading (displacement), tilted glass slide, haemolysis on blood agar, and lipase production. Effects of combination of nitrogen sources (yeast extract and NH4NO3, yeast extract and urea, yeast extract and asparagine, yeast extract and peptone, NaNO3 and peptone, NaNO3 and asparagine, and yeast extract and NaNO3) and carbon sources (glucose, fructose, galactose, cassava peel, soya bran, olive oil, sucrose, crude oil, diesel and glycerol) on biosurfactant production were determined with emulsion stability and surface tension as responses. The bacterium was identified based on phenotypic, microscopic, and biochemical characteristics.

Results: The isolate produced colonies on BH agar containing either naphthalene or hexadecane as sole source of carbon after 48-h incubation. Screening characteristics for the production of biosurfactant by the isolate were as follows: 46% emulsification index, 3.1 cm2 oil displacement, 1.8 cm zone of clearance on tributyrin agar, γ-haemolysis, and positive tilted glass slide. The best carbon source with the highest emulsion stability (51.6%) was fructose whereas the best surface tension reduction (30.85 mN/m) was observed with olive oil as carbon sources after 7 days of incubation. For nitrogen, the combination of yeast extract and NH4NO3 gave the highest emulsion stability (60.7%) and the best surface tension reduction (39.58 mN/m). The data obtained were significant at P<0.05 and the bacterial isolate identified as Stenotrophomonas sp.

Conclusion: This study has demonstrated the ability of the hydrocarbon-utilizing bacterium, Stenotrophomonas sp. to produce biosurfactant, indicated by reduction of surface tension and formation of stable emulsion. This method of biosurfactant production can be further scaled up for industrial purpose. 

Keywords:
Stenotrophomonas sp., hydrocarbon-utilizing bacterium, biosurfactant, surface tension.

Article Details

How to Cite
Ezebuiro, V., Jonathan Otaraku, I., Oruwari, B., & Chijioke Okpokwasili, G. (2019). Effects of Nitrogen and Carbon Sources on Biosurfactant Production by Hydrocarbon-utilizing Stenotrophomonas sp. Microbiology Research Journal International, 29(5), 1-10. https://doi.org/10.9734/mrji/2019/v29i530177
Section
Original Research Article

References

Bustos G, de la Torre N, Moldes AB, Cruz JM, Domínguez JM. Revalorization of hemicellulosic trimming vine shoots hydrolyzates trough continuous production of lactic acid and biosurfactants by L. pentosus. J Food Eng. 2007;78(2):405-412.

Souza EC, Azevedo POS, Domínguez JM, Converti A, Oliveira RPS. Influence of temperature and pH on the production of biosurfactant, bacteriocin and lactic acid by Lactococcus lactis CECT-4434, CyTA - J Food. 2017;15(4):525-530.
DOI: 10.1080/19476337.2017.1306806

Menezes CTB, Barros EC, Rufino RD, Luna JM, Sarubbo LA. Replacing synthetic with microbial surfactants as collectors in the treatment of aqueous effluent produced by acid mine drainage, using the dissolved air flotation technique. Appl Biochem Biotechnol. 2011;163:540–6.
Available:http://dx.doi.org/10.1007/s12010-010-9060-7

Zheng C, Wang M, Wang Y, Huang Z. Optimization of biosurfactant-mediated oil extraction from oil sludge. Bioresour Technol 2012;110:338–42.
Available:http://dx.doi.org/10.1016/j.biortech.2012.01.073

Banat IM, Franzetti A, Gandolfi I, Bestetti G, Martinotti MG, Fracchia L, Smyth TJ, Marchant R. Microorganism in environmental management: Microbes and environment. Appl Microbiol Biotechnol. 2010;87:427–444.

Satpute SK, Banpurkar AG, Dhakephalkar PK, Banat IM, Chopade BA. Methods for investigating biosurfactants and bioemulsifiers: A review. Crit Rev Biotechnol. 2010;30(2):127-144.
Available:https://doi.org/10.3109/07388550903427280 PMID: 20210700

Cortés-Sánchez A, Hernández-Sánchez H, Jaramillo-Flores ME. Biological activity of glycolipids produced by microorganisms: New trends and possible therapeutic alternatives. Microbiol Res. 2013;168:22–32.
DOI: 10.1016/j.micres.2012.07.002

Uzoigwe C, Burgess JG, Ennis CJ, Rahman PK. Bioemulsifiers are not biosurfactants and require different screening approaches. Front Microbiol. 2015;6:245. Available:https://doi.org/10.3389/fmicb.2015.00245

Jhaa SS, Sanket J, Joshia SJ, Geetha SJ. Lipopeptide production by Bacillus subtilis R1 and its possible applications. Braz J Microbiol. 2016;47:955–964.
DOI: 10.1016/j.bjm.2016.07.006

Lyman M, Rubinfeld B, Leif R, Mulcahy H, Dugan L, Souza B. Rhodotorula taiwanensis MD1149 produces hypoacetylated PEFA compounds with increased surface activity compared to Rhodotorula babjevae MD1169. PLoS ONE 2018;13(1):e0190373.
Available:https://doi.org/10.1371/ journal.pone.0190373

Margesin R, Shinner F. Biodegradation and bioremediation of hydrocarbons in extreme environments. Appl Microbiol Biotechnol. 2001;56:650–663.

Nwaguma IV, Chikere CB, Okpokwasili GC. Effect of cultural conditions on biosurfactant production by Candida sp. isolated from the sap of Elaeis guineensis. Biotechnol J Int. 2019;23(3):1-14.
DOI: 10.9734/bji/2019/v23i330079

Bodour AA, Drees KP, Rain M, Maier RM. Distribution of biosurfactant - producing bacteria in undisturbed and contaminated arid southwestern soils. Appl Environ Microbiol. 2003;69(6):3280-3287.

Rahman KS, Rahman T, Lakshmana P, Perummalsamy P, Banat IM. Occurrence of crude oil degrading bacteria in gasoline and diesel station soils. J Basic Microbiol. 2002;42:284-291.

Banat IM, Satpute SK, Cameotra SS, Patil R, Nyayanit NV. Cost effective technologies and renewable substrates for biosurfactants’ production. Front Microbiol. 2014;5:697.
DOI: 10.3389/fmicb.2014.00697

Noha HY, Kathleen ED, David PN, Kristen NS, Roy MK, Michael JM. Comparison of methods to detect biosurfactant production by diverse microorganisms. J Microbiol Meth. 2004;56:339-347.

Asha J, Babu PB, Kirti M, Desphande M. Application of biosurfactant in oil spill management. Oil Spill Conference. 1993; 103-105.

Healy MG, Devine CM, Murphy R. Microbial production of biosurfactants. Resour Conserv Recycl. 1996;18(1–4):41-57.
Available:https://doi.org/10.1016/S0921-3449(96)01167-6

Arino S, Marchal R, Vandecasteele JP. Identification and production of a rhamnolipidic biosurfactant by a Pseudomonas species. Appl Microbiol Biotechnol. 1996; 45:162.
Avaialble:https://doi.org/10.1007/s002530050665

Abouseoud M, Maachi R, Amrane A, Boudergua S, Nabi A. Evaluation of different carbon and nitrogen sources in production of biosurfactant by Pseudomonas fluorescens. Desalination. 2008;223:143–151.

Mittal A, Singh P. Isolation of hydrocarbon degrading bacteria from soils contaminated with crude oil spills. Indian J Expt Biol. 2015;47:760-765.

Mnif S, Sayadi S, Chamkha M. Biodegradative potential and characteriza-tion of a novel aromatic-degrading bacterium isolated from a geothermal oil field under saline and thermophilic conditions. Int Biodeterior Biodegrad. 2014;86:258.

Nwaguma IV, Chikere CB, Okpokwasili GC. Isolation, characterization and application of biosurfactant by Klebsiella pneumonia strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria. Bioresour Bioprocess. 2016; 3:40:1-13.

Nitschke M, Pastore GM. Biosurfactant production by Bacillus subtilis using cassava-processing effluent. Appl Biochem Biotechnol. 2004;112:163–172.

Munguia T, Smith C. A. Surface tension determination through capillary rise and laser diffraction patterns. J Chem Edu. 2001;78(3):343-344.
DOI: 10.1021/ed078p343

Kumar AP, Janardhan A, Radha S, Viswanath B, Narasimha G. Statistical approach to optimize production of biosurfactant by Pseudomonas aeruginosa 2297. 3 Biotech. 2015;5(1):71–79.
DOI: 10.1007/s13205-014-0203-3

Holt JG, Krieg NR, Sneath PHA. Bergey’s manual of determinative bacteriology. Lippincott Williams & Wilkins, USA; 1994.

Palleroni N, Bradbury J. Stenotrophomonas, a new bacterial genus for Xanthomonas maltophilia (Hugh 1980). Int J Syst Bacteriol. 1993;43(3):606–9. DOI: 10.1099/00207713-43-3-606

Chang YT, Lin CY, Chen YH, Hsueh P-R. Update on infections caused by Stenotrophomonas maltophilia with particular attention to resistance mechanisms and therapeutic options. Front Microbiol. 20156:893.
DOI: 10.3389/fmicb.2015.00893

Singh A, Kumar K, Pandey AK. Pyrene degradation by biosurfactant producing bacterium Stenotrophomonas maltophilia. Agric Res. 2015;4:42.
Available:https://doi.org/10.1007/s40003-014-0144-4

Gargouri B, Contreras MD, Ammar S, Segura-Carretero A, Bouaziz M. Biosurfactant production by the crude oil degrading Stenotrophomonas sp. B-2: chemical characterization, biological activities and environmental applications. Environ Sci Pollut Res Int. 2016; 24(4):3769–3779
DOI: 10.1007/s11356-016-8064-4

Larik IA, Qazi MA, Phulpoto AH. Stenotrophomonas maltophilia strain 5DMD: an efficient biosurfactant-producing bacterium for biodegradation of diesel oil and used engine oil. Int J Environ Sci Technol. 2019;16:259.
Available:https://doi.org/10.1007/s13762-018-1666-2

Tripathi V, Gaur VK, Dhiman N, Gautam K, Manickam N. Characterization and properties of the biosurfactant produced by PAH-degrading bacteria isolated from contaminated oily sludge environment. Environmen Sci Pollut Res; 2019.
DOI: 10.1007/s11356-019-05591-3

Makkar RS, Cameotra SS. An update to the use of unconventional substrates for biosurfactant production and their new applications. Appl Microbiol Biotechnol. 2002;58:428–434.

Tan YN, Li Q. Microbial production of rhamnolipids using sugars as carbon sources. Microb Cell Fact. 2018;17: 89. Available:https://doi.org/10.1186/s12934-018-0938-3

Onwosi CO, Odibo FJC. Effects of carbon and nitrogen sources on rhamnolipid biosurfactant production by Pseudomonas nitroreducens isolated from soil. World J Microbiol Biotechnol. 2011;28(3):937–942.
DOI: 10.1007/s11274-011-0891-3

Makkar RS, Cameotra SS. Biosurfactant production by a thermophilic Bacillus subtilis strain. J Ind Microbiol Biotechnol. 1997;18(1):37-42.
DOI: 10.1038/sj.jim.2900349