Prevalence of Multi-Drug Resistance Traits in Probiotic Bacterial Species from Fermented Milk Products in Bangladesh

Main Article Content

Asif Shahriar
Mahenaj Alo
Md. Fahad Hossain
Talha Bin Emran
Md. Zia Uddin
Arkajyoti Paul

Abstract

Aims: Matured nourishment like yogurt and cheese are great wellsprings of probiotic living beings. The present study was carried out for isolation, identification and antibiotic profiling of some important bacteria in, local fermented milk-products sold in the market of Dhaka, Bangladesh.

Methodology: Twenty types of yogurt and fifteen types of cheese samples were collected from two different local market. De Man, Rogosa and Sharpe agar (MRS agar) and Streptococcus thermophilus agar (ST agar) were used for the isolation of probiotic micro-organisms from these different fermented dairy products. Characterization of lactic acid bacteria belonging to the species of Lactobacillus acidophilus, Lactobacillus bulgaricus and Streptococcus thermophilus was done by using morphological feature, Gram staining, biochemical tests (catalase test, some carbohydrate fermentation tests, alpha haemolysis test, starch hydrolysis test, bile salt tolerance test). The antibiotic profiling was performed by utilizing twelve distinct antibiotics: amikacin (10 μg), amoxicillin (15 μg), azithromycin (30 μg), bacitracin (30 μg), chloramphenicol (30 μg), ciprofloxacin (10 μg), erythromycin (30 μg), gentamicin (30 μg), kanamycin (30 μg), penicillin g (30 μg), tetracycline (15 μg), vancomycin (15 μg) to identified the multi-drug resistance of probiotic micro-organisms.

Results: The outcomes demonstrated that specific bacterial species (L. acidophilus, L. bulgaricus and S. thermophilus) were utilized in the production of yogurt and cheese samples, that may have unnecessary antibiotic resistances which can be a risk if they transfer antibiotic resistance genes to pathogenic microbes. Notably, L. bulgaricus was effectively susceptible against bacitracin and penicillin g.

Conclusion: Yogurt and cheese are one kind of probiotic source, made up by using probiotic starter cultures (Lactobacillus spp. and Streptococcus thermophilus) which were identified in this study. Most of the probiotic micro-organisms were resistant against these widely accepted commercial antibiotics. These resistant lactic acid bacteria may transfer their resistance genes to pathogenic microorganisms. It can be a significant cause of concern for human and animal health.

Keywords:
Lactobacillus spp, Streptococcus thermophilus, probiotic food, fermented milk products, yogurt, cheese, drug resistance.

Article Details

How to Cite
Shahriar, A., Alo, M., Hossain, M. F., Bin Emran, T., Uddin, M. Z., & Paul, A. (2019). Prevalence of Multi-Drug Resistance Traits in Probiotic Bacterial Species from Fermented Milk Products in Bangladesh. Microbiology Research Journal International, 29(2), 1-10. https://doi.org/10.9734/mrji/2019/v29i230161
Section
Original Research Article

References

Gismondo MR, Drago L, Lombardi A. Review of probiotics available to modify gastrointestinal flora. Int J Antimicrob Agents. 1999;12:287–292.

Jafari B, Rezaie A, Alizadeh S. Isolation and identification of potentially probiotic bacteria from traditional dairy products of Ardabil region in Iran. Annals Biol Res. 2011;2:311-317.

Wells JM, Mercenier A. Mucosal delivery of therapeutic and prophylactic molecules using lactic acid bacteria. Nature Rev Microbiol. 2008;6:349–362.

Božanić R, Lovković S, Jeličić I. Optimizing fermentation of soymilk with probiotic bacteria. Czech J Food Sci. 2011;29:51-56.

Hummel AS, Hertel C, Holzapfel WH, Franz CMAP. Antibiotic resistances of starter and probiotic strains of lactic acid bacteria. Appl Environ Microbiol. 2007;73: 730–739.

Tserovska L, Stefanova S, Yordanova T. Identification of lactic acid bacteria isolated from Katyk, goats milk and cheese. Journal of Cult Collect. 2002;3:48–52.

Yasmin S, Parveen S, Munna M, Noor R. Detection of Salmonella spp. and microbiological analysis of milk and milk based products available within Dhaka metropolis, Bangladesh. British Microbiol Res J. 2015;5:474–480.

Singh G, Sharma RR. Dominating species of Lactobacilli and Leuconostocs present among the lactic acid bacteria of milk of different cattle’s. Asian J Exp Sci. 2009;23:173-179.

Gadhiya D, Patel A, Prajapati JB. Current trend and future prospective of functional probiotic milk chocolates and related products – a review. Czech J Food Sci. 2015;33:295-301.

Maged S. Bin Masalam, Ahmed Bahieldin, Mona G. Alharbi, Saad Al-Masaudi, Soad K. Al-Jaouni, Steve M. Harakeh, Rashad R. Al-Hindi. Isolation, molecular characterization and probiotic potential of lactic acid bacteria in Saudi raw and fermented milk. Evid Based Complement Alternat Med. 2018;12:7970463.

Federici S, Ciarrocchi F, Campana R, Ciandrini E, Blasi G, Baffone W. Identification and functional traits of lactic acid bacteria isolated from Ciauscolo salami produced in Central Italy. Meat Sci. 2014;98(4):575–584.

Angmo K, Kumari A, Savitri S, Bhalla TC. Probiotic characterization of lactic acid bacteria isolated from fermented foods and beverage of Ladakh. LWT- Food Sci Tech. 2016;66:428–435.

Bezerra TKA, Arcanjo NMDO ARRD. Volatile profile in goat coalho cheese supplemented with probiotic lactic acid bacteria. LWT- Food Sci Tech. 2107;76:209–215.

Kumar A, Kumar D. Characterization of Lactobacillus isolated from dairy samples for probiotic properties. Anaerobe. 2015;33:117–123.

Li P, Luo H, Kong B, Liu Q, Chen C. Formation of red myoglobin derivatives and inhibition of spoilage bacteria in raw meat batters by lactic acid bacteria and Staphylococcus xylosus. LWT- Food Sci Tech. 2016;68:251–257.

Sha Y, Wang L, Liu M, Jiang K, Xin F, Wang B. Effects of lactic acid bacteria and the corresponding supernatant on the survival, growth performance, immune response and disease resistance of Litopenaeus vannamei. Aquaculture. 2016;452:28–36.

Liong MT, Shah NP. Bile salt deconjugation ability, bile salt hydrolase activity and cholesterol co-precipitation ability of Lactobacilli strains. Int Dairy J. 2005;15(4):391–398.

Ouwehand AC, Salminen S, Isolauri E. Probiotics: An overview of beneficial effects. Antonie van Leeuwenhoek-J Microbiol. 2002;82(1–4):279–289.

Giraffa G. Functionality of enterococci in dairy products. Int J Food Microbiol. 2003;88(2-3):15–222.

Mercenier A, Pavan S, Pot B. Probiotics as bio-therapeutic agents: Present knowledge and future prospects. Current Pharma Des. 2003;9(2):175–191.

Strompfová V, Lauková A. Isolation and characterization of faecal bifidobacteria and lactobacilli isolated from dogs and primates. Anaerobe. 2014;29:108–112.

Danielsen M, Wind A. Susceptibility of Lactobacillus spp. to antimicrobial agents. International J Food Microbiol. 2003;82:1–11.

Todorov SD. What bacteriocinogenic lactic acid bacteria do in the milk? In: Academic Press. Raw Milk. 2019;149–174.

Mh F, Sm KR, Rahmana S, Saikota FK, Ch K, Biswasb R. Probiotic properties analysis of isolated lactic acid bacteria from buffalo milk. Arch Clin Microbiol. 2016;7:1.

Yang C, Yu T. Characterization and transfer of antimicrobial resistance in lactic acid bacteria from fermented dairy products in China. J Infect Dev Countr. 2019;13:137–148.

Marjan S, Kanta Das K, Kishore Munshi S, Noor R. Drug-resistant bacterial pathogens in milk and some milk products. Nutri Food Sci. 2014;44:241–248.

de Man JC, Rogosa M, Sharpe ME. A medium for the cultivation of Lactobacilli. J Appl Bacteriol. 1960;23(1):130–135.

Daniel M, Linares-Tom F, O’Callaghan, Paula M, O’Connor, Ross RP, Stanton C. Streptococcus thermophilus APC151 strain is suitable for the manufacture of naturally GABA-enriched bioactive yogurt. Front Microbiol. 2016;7:1876.

Javed I. Characterization of bacteriocin produced by lactic acid bacteria isolated from dairy products [Ph.D. thesis], Quaid-i-Azam University, Islamabad, Pakistan; 2009.

Chahad OB, Bour MEl, Calo-Mata P, Boudabous A, Barros-Velàzquez J. Discovery of novel bio-preservation agents with inhibitory effects on growth of food-borne pathogens and their application to seafood products. Res Microbiol. 2012;163(1):44–54.

Bergey DH, Breed RS, Murray EGD, Hitchens AP. Manual of determinative bacteriology. Manual of Determinative Bacteriology, Fifth Edition; 1939.

Barnard JP, Stinson MW. The alpha-hemolysin of Streptococcus gordonii is hydrogen peroxide. Infect Immunol. 1996;64(9):3853–3857.

Evans JJ, Klesius PH, Shoemaker CA. Starch hydrolysis testing of multiple isolates for rapid differentiation of Streptococcus iniae. Bull Eur Ass Fish Pathol. 2004;24(5):231-239.

Bauer AW, Kirby WM, Sherris JC, Tierch M. Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol. 1966;45(4):493-496.

Ferraro MJ, Craig WA, Dudley MN, Eliopoulos GM, Hecht DW, Hindler J. Performance standards for antimicrobial disk susceptibility testing. Wayne, USA: National Committee for Clinical Laboratory Standards; 2001.

Millette M, Luquet FM, Lacroix M. In vitro growth control of selected pathogens by Lactobacillus acidophilus- and Lactobacillus casei-fermented milk. Lett Appl Microbiol. 2007;44:314–319.

Vescovo M, Morelli L, Bottazzi V. Drug resistance plasmids in Lactobacillus acidophilus and Lactobacillus reuteri. Appl Environl Microbiol. 1982;43:50–56.

Sozzi T, Smiley MB. Antibiotic resistances of yogurt starter cultures Streptococcus thermophilus and Lactobacillus bulgaricus. Appl Environl Microbiol. 1980;40:862–865.

Wolupeck HL, Aparecida Morete C, DallaSanta OR, Bittencourt Luciano F, França Madeira HM, Freitas de Macedo RE. Methods for the evaluation of antibiotic resistance in Lactobacillus isolated from fermented sausages. Ciência Rural; 2017.

Asli Akpinar A, Yerlikaya O, Kiliç S. Antimicrobial activity and antibiotic resistance of Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus strains isolated from Turkish homemade yoghurts. African J Microbiol Res. 2011;5: 675–682.

Sozzi T, Smiley MB. Antibiotic resistances of yogurt starter cultures Streptococcus thermophilus and Lactobacillus bulgaricus. Appl Environl Microbiol. 1980;40:862– 865.

Zhou N, Zhang JX, Fan MT, Wang J, Guo G, Wei XY. Antibiotic resistance of lactic acid bacteria isolated from Chinese yogurts. J Dairy Sci. 2012;95:4775–4783.