Microbiological Quality and Antibiotic Susceptibility Profile of Microorganisms Associated with Stored Vegetables in Port Harcourt

Main Article Content

M. E. Inana
D. N. Ogbonna
S. I. Douglas

Abstract

Aim: Vegetables are good sources of nutrients such as minerals, oil, vitamins and carbohydrates and are used in preparing different delicacies such as soups and salads. This study was aimed at investigating the microbiological quality and antibiotic resistance pattern of microorganisms associated with stored vegetables in raffia baskets.

Methodology: The vegetables were obtained from the Nigerian Stored Products Research institute (NSPRI) farm in Port Harcourt. Ten grams (10 g) each of fresh vegetables were homogenized differently in 90ml of sterile diluent. Aliquot (0.1 ml) of 10-3 and 10-5 dilutions of each vegetable sample was plated on nutrient agar plates and incubated at 37ºC for 24hours. Identified isolates were standardized using the 0.5 McFarland standard. This was done by transferring colonies of the test isolates into sterile 4 mL normal saline and comparing the turbidity of the isolate in the test tubes with the already prepared 0.5 McFarland. The disc diffusion method was used in determining the susceptibility pattern of the microorganisms against the antibiotics. In this method, the standardized inoculums were seeded aseptically on freshly prepared Mueller Hinton agar plates. Whatman discs which have been impregnated with different concentrations of the antibiotics were placed on the seeded plates and incubated at 37ºC for 18-24 hours. 

Results: Zones of clearance or inhibition on plates were recorded. The genera Bacillus, Pseudomonas, Enterococcus, Enterobacter, Bordetella, Staphylococcus, Myroides, Escherichia, Serratia, Micrococcus and Acetobacter were identified as predominant microbes from the vegetables.

Conclusion: Despite the high level of resistance to the antibiotics, Ciprofloxacin and Ofloxacin were the most effective and preferred drugs of choice for treatment of infections arising from the consumption of these vegetables. Adequate heating and blanching of vegetables is required at all times to prevent food poisoning.

Keywords:
Vegetables, storage, microorganisms, antibiotic profile, temperature, humidity.

Article Details

How to Cite
Inana, M. E., Ogbonna, D. N., & Douglas, S. I. (2019). Microbiological Quality and Antibiotic Susceptibility Profile of Microorganisms Associated with Stored Vegetables in Port Harcourt. Microbiology Research Journal International, 29(2), 1-10. https://doi.org/10.9734/mrji/2019/v29i230159
Section
Original Research Article

References

Kemajou TS, Awemu GA, Digban KA, Oshoman CE, Ekundayo OI, Ajugwo AO. Microbiological studies of vegetable leaves sold in Elele Market, Rivers-State, Nigeria. Journal of Transmitted Diseases and Immunity. 2010;1:1-4.

Shukla P, Kumar R, Raib AK. Detection of minerals in green leafy vegetables using laser induced breakdown spectroscopy. Journal of Applied Spectroscopy. 2016; 83(5):872-877.

Aderuipekum CO, Oyetunji OJ. Nutritional values of some tropical vegetables. Journal of Applied Biosciences. 2010;35: 2294-2300.

Ozlem S. Survey of fresh vegetables for nematodes. Journal Association of Analytical Chemistry. 2005;6:613-615.

Feng P, Weagant SD, Gant MA. Enumeration of Escherichia coli and coliform bacteria. In: Marker RI (ed.) revision. AUS Food and Drug Administration College park MD; 2002.

Froder H, Martins CG, Deouza KL, Landgrof M, Franco BD. Minimal processed vegetable salad: Microbial quality evaluation. Journal of Food Production. 2007;70:127-71280.

Mbuwih AA. Preservation and Storage of Leafy Vegetables. Universität Kassel; 2011.

Iheanacho KME, Udebuani AC. Nutritional Composition of some leafy vegetables consumed in Imoo State, Nigeria. Journal of Applied Science and Environmental Management. 2009;13(3):35-38.

Daniel EO, Olisaka FN, Umunna OA, Daodu AA, Odeh HO. Plasmid borne resistance among bacteria isolated from African Salads (“Abacha”). Journal of Advances in Food Science & Technology. 2016;3(3):129-133.

Igbeneghu OA, Abdu AE. Multiple Antibiotic-resistant bacteria on fluted pumpkin leaves, a herb of therapeutic value. Journal of Health Population and Nutrition. 2014;32(2):176-182.

Clarence SY, Nwinyi CO, Chinedu NS. Assessment of bacteriological quality of ready to eat food (Meat pie) in Benin City metropolis, Nigeria. African Journal of Microbiology Research. 2009;3(6):390-395.

Ogbonna DN, Nrior RR, Erheriene BA. Environmental distribution and antibiotic resistance patterns of bacterial isolates from open drainage systems in Port Harcourt Southern Nigeria. Nigerian Journal of Microbiology. 2018;32(1):4241– 4250.

Wemedo SA, Robinson VK. Evaluation of indoor air for bacteria organisms and their antimicrobial susceptibility profile in a Government Health Institution. Journal of Advances in Microbiology. 2018;11(3):1-7.

Ogbonna DN, Inana ME. Characterization and multiple antibiotic resistance of bacterial isolates associated with fish aquaculture in ponds and rivers in Port Harcourt, Nigeria. Journal of Advances in Microbiology. 2018;10(4):1-14.

Clinical and Laboratory Standard Institutes. Performance standards for antimicrobial disk susceptibility tests. CLSI document M100. Clinical and Laboratory Standard Institutes, 28th Edition; 2013.

Mandrell RE, Gorski L, Brandl MT. Attachment of microorganisms to fresh produce. Microbiology of fresh fruits and vegetables, CRC Press. 2006;375-400.

Tandon A, Tay-Kearney ML, Metcalf C, McAllister L. Bacillus circulans endophthalmitis. Clin Experiment Ophthalmol. 2001;29:92-93.

Yoh M, Matsuyama J, Ohnishi M. Importance of Providencia species as a major cause of travellers’ diarrhoea. J Med Microbiol. 2005;54 (11):1077–82.

George PM. Encyclopedia of Foods. Volume 1 Humane Press; Washington. 2003;526.

Tyne DV, Dieye B, Valim C, Daniel RF, Sen FD, Lukem AK, Ndiaye M, Bei AK, Ndiaye VD, Hamiltor EJ, Ndir O, Mboup S, Volkman SK, Wirth DF, Ndiaye D. Changes in drug sensitivity and antimicrobial drug resistance mutations over time among Plasmodium flaciparium parasites in senegal. Malaria Journal. 2013;12:441.

Prescott LM, Harley JP, Klein DA. Microbiology, 7th Edition, McGraw-Hill. New York. 2008;136-139.

Abara AE. Tannin content of Dioscorea bulbifera. Journal of Chemistry Society Nigeria. 2003;28:55-56.

Richard Nyingierefaa Ibiene, Obire Omokaro, Williams Janet Olufunmilayo. Antibiotic resistant pattern of bacteria isolated from Gallus Gallus Domesticus. World Journal of Pharmaceutical and Medical Research. 2019;5(2):01-08.

Azuonwu TE, Ogbonna DN. Resistant genes of microbes associated with abattoir Wastes. Journal of Advances in Medical and Pharmaceutical Sciences 2019;21(2): 1-11.

Akond MA, Alam S, Hassan SMR, Shirin M. Antibiotic resistance of Escherichia Coli isolated from poultry and poultry environment of Bangladesh. Int J Food Safety. 2009;11:19-23.

Wouafo A.M Nzouakeu, Kinfack JA, Fonkoua FC, Ejenguele G, Ninje G, Ngandjio A. Prevalence and antimicrobial resistance of Salmonella serotypes in chickens from retail markets in Yaounde (Cameroon). Microb. Drug. Res. 2010;16: 171-176.