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Staphylococcus aureus is gram-positive cocci that can cause foodborne illness which can be transmitted by chicken meat, both raw and undercooked; consumption of which may cause infection and/or toxicity in consumers. This current study was conducted for the detection of the prevalence of S. aureus in three types of poultry samples which included chicken meat, chicken eggs and droppings. Samples were aseptically collected from different rural and urban areas of 8 districts of Bangladesh in triplicate collection method which was conducted in the Centre for Excellence, Department of Microbiology, Primeasia University, Dhaka, Bangladesh to detect the Multi Drug-Resistant (MDR) S. aureus. Isolation was done by using 7.5% Sodium Chloride broth for enrichment and Mannitol salt agar after enriching samples in Buffer Peptone water. Antibiogram was done by using Kirby Bauer Method on Muller Hinton agar. The current study revealed 68% (17/25), 84% (21/25) and 52% (13/25) of S. aureus in chicken meat, eggs and dropping, respectively. Around 75.47% isolates (40/53) showed 100% similarity with S. aureus from the generated Dendrogram based on biochemical data. Cefixime (62.67%) showed the highest sensitivity against all of the isolates while Penicillin (86.62%) exhibited the highest resistance. Moreover, 59.62% of isolates were resistant to 5 or more drugs (Multidrug-Resistant). Poultry products such as meat and eggs were contaminated with drug-resistant S. aureus which can cause serious health effect to the consumer. The drug-resistant pathogen in droppings helps to indicate the spread of drug-resistant isolates in the environment. Therefore, proper sanitation measures should be taken to ensure the quality of the products during slaughtering, collecting eggs or disposing of poultry litter.
Roy C, Ahmed T, Uddin MA. Micro-biological analysis of poultry feeds along with the demonstration of the antibiotic susceptibility of the isolates and the antibacterial activity of the feeds. Bangladesh J. Microbiol. 2017;34(2):103-107. Available:https://doi.org/10.3329/bjm.v34i2.39620
Danbappa AAR, Alhassan KA, Shah MM. Isolation and identification of microbial contaminants associated with commercial poultry feeds. Journal of Applied and Advanced Research. 2018;3(5):142−147.
Banik A, Abony M, Datta S, Towhid ST. Microbiological quality of ready-to-eat food from Dhaka, Bangladesh. Curr. Res. Nutr Food Sci Jour. 2019;7(1):161-168.
Banik A, Abony M, Datta S, Towhid S. Microbial status and multidrug resistance pattern of pathogenic bacteria isolated from street food in Dhaka City, Bangladesh. Journal of Advances in Micro-biology. 2018;13(1):1-13.
World Health Organization (WHO), Food safety; 2019.
(Accessed 29 March 2012)
Gonçalves-Tenório A, Silva BN, Rodrigues V, Cadavez V, Gonzales-Barron U. Prevalence of pathogens in poultry meat: A meta-analysis of European published surveys. 2018;7(5):69.
Ali Y, Islam MA, Muzahid NH, Sikder MOF, Hossain MA, Marzan LW. Characteri-zation, prevalence and antibiogram study of S. aureus in poultry. Asian Pacific Journal of Tropical Biomedicine. 2017; 7(3):253-256.
Quinn PJ, Carter ME, Markey BK, Carter GR. Staphylococcus species. In: Clinical veterinary microbiology. Edinburgh: Mosby. 2000;118-26.
Onaolapo JA, Igwe JC, Bolaji RO, Adeshina GO, Parom SK. Antibiotics susceptibility profile of Staphylococcus aureus isolated from poultry birds in Kaduna, Nigeria. J Clin Microbiol Anti-microbials. 2017;1:101.
Ruzauskas M, Suziedeliene E, Siugzdiniene R, Seputiene V and Povilonis J. Antimicrobial resistance of Enterococcus spp. spread in poultry products in Lithuania. Journal of Food Safety. 2010; 30(4):902-915.
Durbin CG. Antibiotics in food preservation. Am J Public Health Nations Health. 1956; 46(10):1306-1308.
Herve DT, Kumar G. Prevalence of Staphylococcus aureus in retail chicken meat samples in Jalandhar, Punjab. Research J. Pharma. and Tech. 2017; 10(1):1-5.
Stewart CM. Staphylococcus aureus and staphylococcal enterotoxins. Ch 12 In: Hocking AD (ed) Foodborne micro-organisms of public health significance. 6th ed, Australian Institute of Food Science and Technology (NSW Branch, Sydney. 2003;359–380.
Talarico F, Roccia E, Del Nero I. Prevalence of enterotoxigenic staphylococci in food-handlers in the province of Catanzaro (Italy). Igiene Moderna. 1997;107(2):137-142.
Hatakka M, Bjorkroth KJ, Asplund K, Maki-Petays N, Korkeala HJ. Genotypes and enterotoxicity of Staphylococcus aureus isolated from the hands and nasal cavities of flight catering employees. J Food Prot. 2000;63(11):487-1491.
Figueroa G, Navarrete P, Caro M, Troncoso M, Faundez G. Carriage of enterotoxigenic Staphylococcus aureus in food handlers. Revista Medica De Chile. 2002;130(8):859-864.
Simon SS, Sanjeev S. Prevalence of enterotoxigenic Staphylococcus aureus in fishery products and fish processing factory workers. Food Control. 2007; 18(12):1565-1568.
Kitai S, Shimizu A, Kawano J, Sato E, Nakano C, Kitagawa H, Fujio K, Matsumura, Yasuda R, Inamoto T. Prevalence and characterization of Staphylococcus aureus and entero-toxigenic Staphylococcus aureus in retail raw chicken meat throughout Japan. J Vet Med Sci. 2005;67(3):269-274.
Rahman MA, Rahman A, Islam MA, Alam MM. Multi–drug resistant Staphylococcus aureus isolated from milk, chicken meat, beef and egg in Bangladesh. Research in Agriculture Livestock and Fisheries. 2018; 5(2):175-183. Available:https://doi.org/10.3329/ralf.v5i2.38055
Islam MA, Parveen S, Rahman M, Huq M, Nabi A, Khan ZUM, Ahmed N, Wagenaar JA. Occurrence and characterization of methicillin resistant Staphylococcus aureus in processed raw foods and ready-to-eat foods in an urban setting of a developing country. Front. Microbiol. 2019; 10:503. Available:https://doi.org/10.3389/fmicb.2019.00503
Abdalrahman LS, Stanley A, Wells H, Fakhr MK. Isolation, virulence, and antimicrobial resistance of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin sensitive Staphylococcus aureus (MSSA) strains from Oklahoma retail poultry meats. Int. J. Environ. Res. public Health. 2015;12(6):6148-6161.
Banik A, Mohammad N, Akter T, Fatema K, Abony M. Prevalence, identification and antibiotic susceptibility of Enterococcus Species isolated from chicken and pigeon meat in Gazipur area of Bangladesh. Open Journal of Medical Microbiology. 2018; 8(3):74-83. Available:https://doi.org/10.4236/ojmm.2018.83007
Nazneen Naher Islam, Mahmuda Akter, Zinat Farzana, Abdul Jabber Bin Kader, Inkeyas Uddin AMAM, Zonaed Siddiki K.M. Kamaruddin. Detection of Staphylococcus aureus in frozen chicken rinse through bacteriological and NUC gene specific PCR Methods and their Drug Resistance Patterns in Southern Chittagong, Bangladesh. Research Journal of Micro-biology. 2014;9:251-264.
Abony M, Banik A, Shishir MA, Akter NJ, Uddin ME, Datta S. Physico-chemical Characterization of Indigenous Streptomyces and Influence of pH on antimicrobial activity. Microbial Bioactives. 2018;1(2):059-067.
Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology. 1996;45: 493-496.
Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement. CLSI. s.l. : PA: Clinical and Laboratory Standards Institute, Vols. CLSI document M100-S22; 2012.
Steel ROD, Torrie JH. Principles and procedures of statistics. A Biometrical Approach. McGraw-Hill, New York. 1980; 633.
Andrew E, Tania C, Jordan B, Cindy M, Lindsey W, Kimberly P, Jeffrey T, Jolene B, Elizabeth M, David M, Paul S, Lance B. Multidrug-resistant Staphylococcus aureus in US meat and poultry, center for microbial genetics and genomics. Northern Arizona University Journal of Clinical Infectious Diseases. 2011;52(1):1-4.
Lin J, Yeh KS, Liu HT, Lin JH. Staphylococcus aureus isolated from pork and chicken carcasses in Taiwan: Prevalence and antimicrobial susceptibility. J. Food Prot. 2009;72(3):608-611.
Hassan Momtaz, Farhad Safarpoor Dehkordi, Ebrahim Rahimi, Amin Asgarifar, Mahmood Momeni. Virulence genes and antimicrobial resistance profiles of Staphylococcus aureus isolated from chicken meat in Isfahan province, Iran. The Journal of Applied Poultry Research. 2013; 22(4):913–921. Available:https://doi.org/10.3382/japr.2012-00673
Akbar A, Anal AK. Prevalence and anti-biogram study of Salmonella and Staphylococcus aureus in poultry meat. Asian Pac J. Trop Biomed. 2013;3(2):163-168.
Kadariya J, Smith TC, Thapaliya D. Staphylococcus aureus and staphylococcal food-borne disease: An ongoing challenge in public health. BioMed Res Int. 2014; 2014;827965.
Scallan E, Hoekstra RM, Angulo FJ,Tauxe RV, Widdowson MA, Roy SL, Jones JL, Griffin PM. Foodborne illness acquired in the United States—Major pathogens. Emerg Infect Dis. 2011;17(1):7-15.
Datta S, Akter A, Shah IG, Fatema K, Islam TH, Bandyopadhyay A, Khan ZUM, Biswas D. Microbiological quality assessment of raw meat and meat products, and antibiotic susceptibility of isolated S. aureus. Agriculture, Food and Analytical Bacteriology. 2012;2(3):187-194.
Ema FA, Arif M, Islam MA, Khatun MM. Isolation and identification of duck egg-borne bacteria and their antibiogram profile. Journal of Advanced Veterinary and Animal Research. 2018;5(2):110-116.
Fardows J, Siddique A, Moureen A, Islam T, Farhana N, Naheen C. Isolation and identification of pathogenic gram-positive bacteria from egg shell of hen and to see their antimicrobial susceptibility pattern. Journal of Enam Medical College. 2016; 6(1):15-18.