Characterization of Antifungal Volatile Organic Compounds Produced by Bacterial Endophytes against Fusarium oxysporum through GC-MS Analysis

Main Article Content

P. Muthulakshmi
Suganya Thangavel
Parthasarathy Seethapathy

Abstract

Banana (Musa spp.) is one of the most important fruit crops cultivated throughout the world. Fusarium wilt caused by the fungus, Fusarium oxysporum f. sp. cubense (E.F. Smith) Snyder & Hansen (Foc) causes significant threats to banana production. Endophytic bacteria play a significant role in plant protection against soil borne plant pathogens and promote overall productivity of agricultural ecosystems. Secondary metabolites produced by several species of Bacillus have been found to possess antibacterial or antifungal activity against different isolates of Fusarium oxysporum f. sp. cubense.  These were identified by crude tests using agar gel diffusion and GC-MS analysis of the fungal crude extracts. Understanding of the metabolites produced by endophytes is also important in the context of biological control of soil-borne diseases of banana. GC-MS analysis of crude antibiotic extracts of bacterial endophytic isolates viz., GNBS3, PVBS3, NPBS4, KVBS4, BS1, revealed DL-Proline,5 Oxo  as the major compound. Having both antifungal and antibacterial activity.

Keywords:
Banana, Bacillus, panama wilt, secondary metabolites, GC-MS analysis.

Article Details

How to Cite
Muthulakshmi, P., Thangavel, S., & Seethapathy, P. (2019). Characterization of Antifungal Volatile Organic Compounds Produced by Bacterial Endophytes against Fusarium oxysporum through GC-MS Analysis. Microbiology Research Journal International, 29(1), 1-9. https://doi.org/10.9734/mrji/2019/v29i130153
Section
Original Research Article

References

Mohapatra D, Mishra S, Sutar N. Banana and its by-product utilisation: An overview. Journal of Scientific and Industrial Research. 2010;69(5):323- 329.

Ploetz RC, Minnesota US, Stover RH, Nel B, Steinberg C, Labuschagne N, Cornelissen BJC. Fusarium Wilt of Banana. The potential of nonpathogenic Fusarium oxysporum and other biological control organisms for suppressing Fusarium wilt of banana. Plant Pathology. 1997;55:217-223.

Stover RH. Studies on Fusarium wilt of bananas. IX Competitive saprophytic ability of Fusarium oxysporum f. sp. cubense. Canadian Journal of Botany. 1962;40:1473-1481.

Lakshmanan P, Selvaraj P, Mohan S. Efficacy of different methods for the control of Panama disease. Tropical Pest Management. 2008;33(4):373– 374.
DOI: 10.1080/09670878709371187

Ploetz RC. Fusarium wilt of banana. Phytopathology. 2015;105:1521-1522.

Olivain C, Alabouvette C, Steinberg C. Production of a mixed inoculum of Fusarium oxysporum Fo47 and Pseudomonas fluorescens C7 to control Fusarium diseases. Biocontrol Science and Technology. 2004;14(3):227-238.

Ahsan T, Chen J, Zhao X, Irfan M, and Wu Y. Extraction and identification of bioactive compounds (eicosane and dibutyl phthalate) produced by Streptomyces strain KX852460 for the biological control of Rhizoctonia solani AG3 strain KX852461 to control target spot disease in tobacco leaf. AMB Express. 2017;7(1):54. Doi: 10.1186/s13568-017-0351

Stead DE, Elphinstone JG, Weller S, Smith N, Hennessy J. Modern methods for characterising, identifying and detecting bacteria associated with plants. 1999.
DOI: 10.17660/ActaHortic. 2000;530.4

Al-Ani L, Amir GH. Biocontrol of Fusarium wilt of Banana by Trichoderma spp. 8th Postgraduate PPSKH Colloqium at: School of Biological Sciences, USM. 2013;3.

Ongena M, Jacques P. Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends in Microbiology. 2008;16(3):115-125.

King EO, Ward MK, Reney DE. Two samples media for the demonstration of pyocyanin and fluorescein. Journal of Laboratory and Clinical Medicine. 1954;44:301-307.

Prapagdee B, Tharasaithong L, Nanthaphot R, Paisitwiroj C.Efficacy of crude extract of antifungal compounds produced from bacillus subtilis on prevention of anthrancnose disease in Dendrobium Orchid. Environment Asia. 2012;5:32-38.

Yasmeen S, Muvva V, Munaganti RK. Isolation and characterization of bioactive Streptomyces from mangrove eco- system of Machilipatnam, Krishna District, Andhra Pradesh. Asian Journal of Pharmaceutical and Clinical Research. 2017;9(9):258.
DOI: 10.22159/ajpcr.2016.v9s3.149.

Islam MR, Jeong YT, Lee YC, Song CH. Isolation and identification of antifungal compounds from Bacillus subtilis C9 inhibiting the growth of plant pathogenic fungi. Mycobiology. 2012;40:59-66.
DOI: 10.5941/MYCO.2012.40.1.059

Melo FMPD, Fiore MF, Moraes LABD, Silva-Stenico ME, Scramin S, Teixeira MDA, Melo IS D. Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4A. Scientia Agricola. 2009;66(5):583-592.

Gomez KA, Gomez AA. Statistical procedure for Agricultural Research. John Wiley and Sons, New York; 1984.

Yavuz B, Arslan U. Antifungal activity of some plants extracts against phytopathogenic fungi. Journal of Food Agriculture and Environment. 2013:11; 704–709.
DOI: 10.17485/ ijst/2009/v2i4/29438

Etminani F, Harighi B. Isolation and identification of endophytic bacteria with plant growth promoting activity and biocontrol potential from wild pistachio trees. Plant Pathology Journal. 2018;34:208–217.
DOI: 10.5423/PPJ.OA.07.2017.0158

Rajeswari G, Murugan M, Mohan VR. GC-MS analysis of bioactive components of Hugonia mystax l. (linaceae). Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2012;3:301–308.